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Rapid loss of seed viability in ex situ conserved wheat and barley at 4°C as compared to −20°C storage

Genebanks aim to optimize their storage conditions in order to postpone seed ageing as long as possible. As most genebanks have a relatively short life history, empirical data about seed longevity during ex situ storage are almost absent. Based on seed characteristics, theoretical predictions indica...

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Autores principales: van Treuren, Rob, Bas, Noor, Kodde, Jan, Groot, Steven P C, Kik, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016650/
https://www.ncbi.nlm.nih.gov/pubmed/29977565
http://dx.doi.org/10.1093/conphys/coy033
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author van Treuren, Rob
Bas, Noor
Kodde, Jan
Groot, Steven P C
Kik, Chris
author_facet van Treuren, Rob
Bas, Noor
Kodde, Jan
Groot, Steven P C
Kik, Chris
author_sort van Treuren, Rob
collection PubMed
description Genebanks aim to optimize their storage conditions in order to postpone seed ageing as long as possible. As most genebanks have a relatively short life history, empirical data about seed longevity during ex situ storage are almost absent. Based on seed characteristics, theoretical predictions indicate that cereal seeds can be stored without substantial loss of viability for time periods exceeding 100 years, even under temperatures of a few degrees above zero. Here we present the results of a germination study in wheat and barley, comparing genebank seed samples maintained at different temperatures for 23–33 years. Wheat and barley seed samples stored at −20°C showed a mean germination of 94% and 90%, respectively, indicating no loss of the initial viability determined for the accessions prior to introduction in the collection. Seed samples maintained at 4°C showed a mean germination of 62% for wheat and 75% for barley. In addition to the observed loss of viability, the 4°C samples also showed a loss in vigour as the time period to reach their final germination was about twice as long compared to the −20°C samples. A subset of the wheat accessions tested in 2011 were retested in 2017, showing further reduction in mean germination to 35% for the 4°C samples, while the −20°C samples remained stable at 95%. Several 4°C samples were even close to a complete loss of viability. Considering that wheat and barley are generally regarded as good maintainers, the rapid loss of seed viability observed in the present study indicates that the ex situ seed storage of genetic resources at 4°C should be treated with caution by genebanks, particularly when used for long-term conservation.
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spelling pubmed-60166502018-07-05 Rapid loss of seed viability in ex situ conserved wheat and barley at 4°C as compared to −20°C storage van Treuren, Rob Bas, Noor Kodde, Jan Groot, Steven P C Kik, Chris Conserv Physiol Research Article Genebanks aim to optimize their storage conditions in order to postpone seed ageing as long as possible. As most genebanks have a relatively short life history, empirical data about seed longevity during ex situ storage are almost absent. Based on seed characteristics, theoretical predictions indicate that cereal seeds can be stored without substantial loss of viability for time periods exceeding 100 years, even under temperatures of a few degrees above zero. Here we present the results of a germination study in wheat and barley, comparing genebank seed samples maintained at different temperatures for 23–33 years. Wheat and barley seed samples stored at −20°C showed a mean germination of 94% and 90%, respectively, indicating no loss of the initial viability determined for the accessions prior to introduction in the collection. Seed samples maintained at 4°C showed a mean germination of 62% for wheat and 75% for barley. In addition to the observed loss of viability, the 4°C samples also showed a loss in vigour as the time period to reach their final germination was about twice as long compared to the −20°C samples. A subset of the wheat accessions tested in 2011 were retested in 2017, showing further reduction in mean germination to 35% for the 4°C samples, while the −20°C samples remained stable at 95%. Several 4°C samples were even close to a complete loss of viability. Considering that wheat and barley are generally regarded as good maintainers, the rapid loss of seed viability observed in the present study indicates that the ex situ seed storage of genetic resources at 4°C should be treated with caution by genebanks, particularly when used for long-term conservation. Oxford University Press 2018-06-25 /pmc/articles/PMC6016650/ /pubmed/29977565 http://dx.doi.org/10.1093/conphys/coy033 Text en © The Author(s) 2018. Published by Oxford University Press and the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
van Treuren, Rob
Bas, Noor
Kodde, Jan
Groot, Steven P C
Kik, Chris
Rapid loss of seed viability in ex situ conserved wheat and barley at 4°C as compared to −20°C storage
title Rapid loss of seed viability in ex situ conserved wheat and barley at 4°C as compared to −20°C storage
title_full Rapid loss of seed viability in ex situ conserved wheat and barley at 4°C as compared to −20°C storage
title_fullStr Rapid loss of seed viability in ex situ conserved wheat and barley at 4°C as compared to −20°C storage
title_full_unstemmed Rapid loss of seed viability in ex situ conserved wheat and barley at 4°C as compared to −20°C storage
title_short Rapid loss of seed viability in ex situ conserved wheat and barley at 4°C as compared to −20°C storage
title_sort rapid loss of seed viability in ex situ conserved wheat and barley at 4°c as compared to −20°c storage
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016650/
https://www.ncbi.nlm.nih.gov/pubmed/29977565
http://dx.doi.org/10.1093/conphys/coy033
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