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High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source

In the field of X-ray microcomputed tomography (μCT) there is a growing need to reduce acquisition times at high spatial resolution (approximate micrometers) to facilitate in vivo and high-throughput operations. The state of the art represented by synchrotron light sources is not practical for certa...

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Autores principales: Cole, Jason M., Symes, Daniel R., Lopes, Nelson C., Wood, Jonathan C., Poder, Kristjan, Alatabi, Saleh, Botchway, Stanley W., Foster, Peta S., Gratton, Sarah, Johnson, Sara, Kamperidis, Christos, Kononenko, Olena, De Lazzari, Michael, Palmer, Charlotte A. J., Rusby, Dean, Sanderson, Jeremy, Sandholzer, Michael, Sarri, Gianluca, Szoke-Kovacs, Zsombor, Teboul, Lydia, Thompson, James M., Warwick, Jonathan R., Westerberg, Henrik, Hill, Mark A., Norris, Dominic P., Mangles, Stuart P. D., Najmudin, Zulfikar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016801/
https://www.ncbi.nlm.nih.gov/pubmed/29871946
http://dx.doi.org/10.1073/pnas.1802314115
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author Cole, Jason M.
Symes, Daniel R.
Lopes, Nelson C.
Wood, Jonathan C.
Poder, Kristjan
Alatabi, Saleh
Botchway, Stanley W.
Foster, Peta S.
Gratton, Sarah
Johnson, Sara
Kamperidis, Christos
Kononenko, Olena
De Lazzari, Michael
Palmer, Charlotte A. J.
Rusby, Dean
Sanderson, Jeremy
Sandholzer, Michael
Sarri, Gianluca
Szoke-Kovacs, Zsombor
Teboul, Lydia
Thompson, James M.
Warwick, Jonathan R.
Westerberg, Henrik
Hill, Mark A.
Norris, Dominic P.
Mangles, Stuart P. D.
Najmudin, Zulfikar
author_facet Cole, Jason M.
Symes, Daniel R.
Lopes, Nelson C.
Wood, Jonathan C.
Poder, Kristjan
Alatabi, Saleh
Botchway, Stanley W.
Foster, Peta S.
Gratton, Sarah
Johnson, Sara
Kamperidis, Christos
Kononenko, Olena
De Lazzari, Michael
Palmer, Charlotte A. J.
Rusby, Dean
Sanderson, Jeremy
Sandholzer, Michael
Sarri, Gianluca
Szoke-Kovacs, Zsombor
Teboul, Lydia
Thompson, James M.
Warwick, Jonathan R.
Westerberg, Henrik
Hill, Mark A.
Norris, Dominic P.
Mangles, Stuart P. D.
Najmudin, Zulfikar
author_sort Cole, Jason M.
collection PubMed
description In the field of X-ray microcomputed tomography (μCT) there is a growing need to reduce acquisition times at high spatial resolution (approximate micrometers) to facilitate in vivo and high-throughput operations. The state of the art represented by synchrotron light sources is not practical for certain applications, and therefore the development of high-brightness laboratory-scale sources is crucial. We present here imaging of a fixed embryonic mouse sample using a compact laser–plasma-based X-ray light source and compare the results to images obtained using a commercial X-ray μCT scanner. The radiation is generated by the betatron motion of electrons inside a dilute and transient plasma, which circumvents the flux limitations imposed by the solid or liquid anodes used in conventional electron-impact X-ray tubes. This X-ray source is pulsed (duration <30 fs), bright (>10(10) photons per pulse), small (diameter <1 μm), and has a critical energy >15 keV. Stable X-ray performance enabled tomographic imaging of equivalent quality to that of the μCT scanner, an important confirmation of the suitability of the laser-driven source for applications. The X-ray flux achievable with this approach scales with the laser repetition rate without compromising the source size, which will allow the recording of high-resolution μCT scans in minutes.
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spelling pubmed-60168012018-06-26 High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source Cole, Jason M. Symes, Daniel R. Lopes, Nelson C. Wood, Jonathan C. Poder, Kristjan Alatabi, Saleh Botchway, Stanley W. Foster, Peta S. Gratton, Sarah Johnson, Sara Kamperidis, Christos Kononenko, Olena De Lazzari, Michael Palmer, Charlotte A. J. Rusby, Dean Sanderson, Jeremy Sandholzer, Michael Sarri, Gianluca Szoke-Kovacs, Zsombor Teboul, Lydia Thompson, James M. Warwick, Jonathan R. Westerberg, Henrik Hill, Mark A. Norris, Dominic P. Mangles, Stuart P. D. Najmudin, Zulfikar Proc Natl Acad Sci U S A Physical Sciences In the field of X-ray microcomputed tomography (μCT) there is a growing need to reduce acquisition times at high spatial resolution (approximate micrometers) to facilitate in vivo and high-throughput operations. The state of the art represented by synchrotron light sources is not practical for certain applications, and therefore the development of high-brightness laboratory-scale sources is crucial. We present here imaging of a fixed embryonic mouse sample using a compact laser–plasma-based X-ray light source and compare the results to images obtained using a commercial X-ray μCT scanner. The radiation is generated by the betatron motion of electrons inside a dilute and transient plasma, which circumvents the flux limitations imposed by the solid or liquid anodes used in conventional electron-impact X-ray tubes. This X-ray source is pulsed (duration <30 fs), bright (>10(10) photons per pulse), small (diameter <1 μm), and has a critical energy >15 keV. Stable X-ray performance enabled tomographic imaging of equivalent quality to that of the μCT scanner, an important confirmation of the suitability of the laser-driven source for applications. The X-ray flux achievable with this approach scales with the laser repetition rate without compromising the source size, which will allow the recording of high-resolution μCT scans in minutes. National Academy of Sciences 2018-06-19 2018-06-05 /pmc/articles/PMC6016801/ /pubmed/29871946 http://dx.doi.org/10.1073/pnas.1802314115 Text en Copyright © 2018 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/ This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Physical Sciences
Cole, Jason M.
Symes, Daniel R.
Lopes, Nelson C.
Wood, Jonathan C.
Poder, Kristjan
Alatabi, Saleh
Botchway, Stanley W.
Foster, Peta S.
Gratton, Sarah
Johnson, Sara
Kamperidis, Christos
Kononenko, Olena
De Lazzari, Michael
Palmer, Charlotte A. J.
Rusby, Dean
Sanderson, Jeremy
Sandholzer, Michael
Sarri, Gianluca
Szoke-Kovacs, Zsombor
Teboul, Lydia
Thompson, James M.
Warwick, Jonathan R.
Westerberg, Henrik
Hill, Mark A.
Norris, Dominic P.
Mangles, Stuart P. D.
Najmudin, Zulfikar
High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source
title High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source
title_full High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source
title_fullStr High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source
title_full_unstemmed High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source
title_short High-resolution μCT of a mouse embryo using a compact laser-driven X-ray betatron source
title_sort high-resolution μct of a mouse embryo using a compact laser-driven x-ray betatron source
topic Physical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016801/
https://www.ncbi.nlm.nih.gov/pubmed/29871946
http://dx.doi.org/10.1073/pnas.1802314115
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