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Food anticipatory activity on a calorie-restricted diet is independent of Sirt1

A number of studies have demonstrated that the Sirtuin family member, Sirt1, is a key integrator of growth, metabolism, and lifespan. Sirt1 directly interacts with and deacetylates key regulators of the circadian clock, positioning it to be an important link between feeding and circadian rhythms. In...

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Autores principales: Assali, Dina R., Hsu, Cynthia T., Gunapala, Keith M., Aguayo, Antonio, McBurney, Michael, Steele, Andrew D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016907/
https://www.ncbi.nlm.nih.gov/pubmed/29940007
http://dx.doi.org/10.1371/journal.pone.0199586
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author Assali, Dina R.
Hsu, Cynthia T.
Gunapala, Keith M.
Aguayo, Antonio
McBurney, Michael
Steele, Andrew D.
author_facet Assali, Dina R.
Hsu, Cynthia T.
Gunapala, Keith M.
Aguayo, Antonio
McBurney, Michael
Steele, Andrew D.
author_sort Assali, Dina R.
collection PubMed
description A number of studies have demonstrated that the Sirtuin family member, Sirt1, is a key integrator of growth, metabolism, and lifespan. Sirt1 directly interacts with and deacetylates key regulators of the circadian clock, positioning it to be an important link between feeding and circadian rhythms. In fact, one study suggests that Sirt1 is necessary for behavioral anticipation of limited daily food availability, a circadian process termed food anticipatory activity (FAA). In their study, mice overexpressing Sirt1 had enhanced FAA, while mice lacking Sirt1 had little to no FAA. Based on the supposition that Sirt1 was indeed required for FAA, we sought to use Sirt1 deletion to map the neural circuitry responsible for FAA. We began by inactivating Sirt1 using the cell-type specific Cre-driver lines proopiomelanocortin, but after observing no effect on body weight loss or FAA we then moved on to more broadly neuronal Cre drivers Ca2+/calmodulin-dependent protein kinase II and nestin. As neither of these neuronal deletions of Sirt1 had impaired FAA, we then tested 1) a broad postnatal tamoxifen-inducible deletion, 2) a complete, developmental knockout of Sirt1, and 3) a gene replacement, catalytically inactive, form of Sirt1; but all of these mice had FAA similar to controls. Therefore, our findings suggest that FAA is completely independent of Sirt1.
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spelling pubmed-60169072018-07-07 Food anticipatory activity on a calorie-restricted diet is independent of Sirt1 Assali, Dina R. Hsu, Cynthia T. Gunapala, Keith M. Aguayo, Antonio McBurney, Michael Steele, Andrew D. PLoS One Research Article A number of studies have demonstrated that the Sirtuin family member, Sirt1, is a key integrator of growth, metabolism, and lifespan. Sirt1 directly interacts with and deacetylates key regulators of the circadian clock, positioning it to be an important link between feeding and circadian rhythms. In fact, one study suggests that Sirt1 is necessary for behavioral anticipation of limited daily food availability, a circadian process termed food anticipatory activity (FAA). In their study, mice overexpressing Sirt1 had enhanced FAA, while mice lacking Sirt1 had little to no FAA. Based on the supposition that Sirt1 was indeed required for FAA, we sought to use Sirt1 deletion to map the neural circuitry responsible for FAA. We began by inactivating Sirt1 using the cell-type specific Cre-driver lines proopiomelanocortin, but after observing no effect on body weight loss or FAA we then moved on to more broadly neuronal Cre drivers Ca2+/calmodulin-dependent protein kinase II and nestin. As neither of these neuronal deletions of Sirt1 had impaired FAA, we then tested 1) a broad postnatal tamoxifen-inducible deletion, 2) a complete, developmental knockout of Sirt1, and 3) a gene replacement, catalytically inactive, form of Sirt1; but all of these mice had FAA similar to controls. Therefore, our findings suggest that FAA is completely independent of Sirt1. Public Library of Science 2018-06-25 /pmc/articles/PMC6016907/ /pubmed/29940007 http://dx.doi.org/10.1371/journal.pone.0199586 Text en © 2018 Assali et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Assali, Dina R.
Hsu, Cynthia T.
Gunapala, Keith M.
Aguayo, Antonio
McBurney, Michael
Steele, Andrew D.
Food anticipatory activity on a calorie-restricted diet is independent of Sirt1
title Food anticipatory activity on a calorie-restricted diet is independent of Sirt1
title_full Food anticipatory activity on a calorie-restricted diet is independent of Sirt1
title_fullStr Food anticipatory activity on a calorie-restricted diet is independent of Sirt1
title_full_unstemmed Food anticipatory activity on a calorie-restricted diet is independent of Sirt1
title_short Food anticipatory activity on a calorie-restricted diet is independent of Sirt1
title_sort food anticipatory activity on a calorie-restricted diet is independent of sirt1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016907/
https://www.ncbi.nlm.nih.gov/pubmed/29940007
http://dx.doi.org/10.1371/journal.pone.0199586
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