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A high precision method for length-based separation of carbon nanotubes using bio-conjugation, SDS-PAGE and silver staining
Parametric separation of carbon nanotubes, especially based on their length is a challenge for a number of nano-tech researchers. We demonstrate a method to combine bio-conjugation, SDS-PAGE, and silver staining in order to separate carbon nanotubes on the basis of length. Egg-white lysozyme, conjug...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016930/ https://www.ncbi.nlm.nih.gov/pubmed/29939999 http://dx.doi.org/10.1371/journal.pone.0197972 |
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author | Borzooeian, Zahra Taslim, Mohammad E. Ghasemi, Omid Rezvani, Saina Borzooeian, Giti Nourbakhsh, Amirhasan |
author_facet | Borzooeian, Zahra Taslim, Mohammad E. Ghasemi, Omid Rezvani, Saina Borzooeian, Giti Nourbakhsh, Amirhasan |
author_sort | Borzooeian, Zahra |
collection | PubMed |
description | Parametric separation of carbon nanotubes, especially based on their length is a challenge for a number of nano-tech researchers. We demonstrate a method to combine bio-conjugation, SDS-PAGE, and silver staining in order to separate carbon nanotubes on the basis of length. Egg-white lysozyme, conjugated covalently onto the single-walled carbon nanotubes surfaces using carbodiimide method. The proposed conjugation of a biomolecule onto the carbon nanotubes surfaces is a novel idea and a significant step forward for creating an indicator for length-based carbon nanotubes separation. The conjugation step was followed by SDS-PAGE and the nanotube fragments were precisely visualized using silver staining. This high precision, inexpensive, rapid and simple separation method obviates the need for centrifugation, additional chemical analyses, and expensive spectroscopic techniques such as Raman spectroscopy to visualize carbon nanotube bands. In this method, we measured the length of nanotubes using different image analysis techniques which is based on a simplified hydrodynamic model. The method has high precision and resolution and is effective in separating the nanotubes by length which would be a valuable quality control tool for the manufacture of carbon nanotubes of specific lengths in bulk quantities. To this end, we were also able to measure the carbon nanotubes of different length, produced from different sonication time intervals. |
format | Online Article Text |
id | pubmed-6016930 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-60169302018-07-07 A high precision method for length-based separation of carbon nanotubes using bio-conjugation, SDS-PAGE and silver staining Borzooeian, Zahra Taslim, Mohammad E. Ghasemi, Omid Rezvani, Saina Borzooeian, Giti Nourbakhsh, Amirhasan PLoS One Research Article Parametric separation of carbon nanotubes, especially based on their length is a challenge for a number of nano-tech researchers. We demonstrate a method to combine bio-conjugation, SDS-PAGE, and silver staining in order to separate carbon nanotubes on the basis of length. Egg-white lysozyme, conjugated covalently onto the single-walled carbon nanotubes surfaces using carbodiimide method. The proposed conjugation of a biomolecule onto the carbon nanotubes surfaces is a novel idea and a significant step forward for creating an indicator for length-based carbon nanotubes separation. The conjugation step was followed by SDS-PAGE and the nanotube fragments were precisely visualized using silver staining. This high precision, inexpensive, rapid and simple separation method obviates the need for centrifugation, additional chemical analyses, and expensive spectroscopic techniques such as Raman spectroscopy to visualize carbon nanotube bands. In this method, we measured the length of nanotubes using different image analysis techniques which is based on a simplified hydrodynamic model. The method has high precision and resolution and is effective in separating the nanotubes by length which would be a valuable quality control tool for the manufacture of carbon nanotubes of specific lengths in bulk quantities. To this end, we were also able to measure the carbon nanotubes of different length, produced from different sonication time intervals. Public Library of Science 2018-06-25 /pmc/articles/PMC6016930/ /pubmed/29939999 http://dx.doi.org/10.1371/journal.pone.0197972 Text en © 2018 Borzooeian et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Borzooeian, Zahra Taslim, Mohammad E. Ghasemi, Omid Rezvani, Saina Borzooeian, Giti Nourbakhsh, Amirhasan A high precision method for length-based separation of carbon nanotubes using bio-conjugation, SDS-PAGE and silver staining |
title | A high precision method for length-based separation of carbon nanotubes using bio-conjugation, SDS-PAGE and silver staining |
title_full | A high precision method for length-based separation of carbon nanotubes using bio-conjugation, SDS-PAGE and silver staining |
title_fullStr | A high precision method for length-based separation of carbon nanotubes using bio-conjugation, SDS-PAGE and silver staining |
title_full_unstemmed | A high precision method for length-based separation of carbon nanotubes using bio-conjugation, SDS-PAGE and silver staining |
title_short | A high precision method for length-based separation of carbon nanotubes using bio-conjugation, SDS-PAGE and silver staining |
title_sort | high precision method for length-based separation of carbon nanotubes using bio-conjugation, sds-page and silver staining |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6016930/ https://www.ncbi.nlm.nih.gov/pubmed/29939999 http://dx.doi.org/10.1371/journal.pone.0197972 |
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