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HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex
Human telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017123/ https://www.ncbi.nlm.nih.gov/pubmed/29361764 http://dx.doi.org/10.3390/molecules23010237 |
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author | Liu, Xiao Xu, Yan |
author_facet | Liu, Xiao Xu, Yan |
author_sort | Liu, Xiao |
collection | PubMed |
description | Human telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1). Here, we report on a detailed analysis of hnRNPA1 binding to telomere RNA G-quadruplexes with a group of loop variants using an electrophoretic mobility shift assay (EMSA) and circular dichroism (CD) spectroscopy. We found that the hnRNPA1 binds to RNA G-quadruplexes with the 2’-O-methyl and DNA loops, but fails to bind with the abasic RNA and DNA loops. These results suggested that hnRNPA1 binds to the loop of the RNA G-quadruplex by recognizing the base of the loop’s nucleotides. The observation provides the first evidence that the base of the loop’s nucleotides is a key factor for hnRNPA1 specifically recognizing the RNA G-quadruplex. |
format | Online Article Text |
id | pubmed-6017123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60171232018-11-13 HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex Liu, Xiao Xu, Yan Molecules Article Human telomere RNA performs various cellular functions, such as telomere length regulation, heterochromatin formation, and end protection. We recently demonstrated that the loops in the RNA G-quadruplex are important in the interaction of telomere RNA with heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1). Here, we report on a detailed analysis of hnRNPA1 binding to telomere RNA G-quadruplexes with a group of loop variants using an electrophoretic mobility shift assay (EMSA) and circular dichroism (CD) spectroscopy. We found that the hnRNPA1 binds to RNA G-quadruplexes with the 2’-O-methyl and DNA loops, but fails to bind with the abasic RNA and DNA loops. These results suggested that hnRNPA1 binds to the loop of the RNA G-quadruplex by recognizing the base of the loop’s nucleotides. The observation provides the first evidence that the base of the loop’s nucleotides is a key factor for hnRNPA1 specifically recognizing the RNA G-quadruplex. MDPI 2018-01-22 /pmc/articles/PMC6017123/ /pubmed/29361764 http://dx.doi.org/10.3390/molecules23010237 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Xiao Xu, Yan HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title | HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title_full | HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title_fullStr | HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title_full_unstemmed | HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title_short | HnRNPA1 Specifically Recognizes the Base of Nucleotide at the Loop of RNA G-Quadruplex |
title_sort | hnrnpa1 specifically recognizes the base of nucleotide at the loop of rna g-quadruplex |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017123/ https://www.ncbi.nlm.nih.gov/pubmed/29361764 http://dx.doi.org/10.3390/molecules23010237 |
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