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β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation

In the present study we evaluated the anti-angiogenic activities of β-escin (the major active compound of Aesculus hippocastanum L. seeds). Human umbilical-vein endothelial cells (HUVECs) were used as an in vitro model for studying the molecular mechanism underlying the anti-angiogenic effect of β-e...

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Autores principales: Varinská, Lenka, Fáber, Lenka, Kello, Martin, Petrovová, Eva, Balážová, Ľudmila, Solár, Peter, Čoma, Matúš, Urdzík, Peter, Mojžiš, Ján, Švajdlenka, Emil, Mučaji, Pavel, Gál, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017140/
https://www.ncbi.nlm.nih.gov/pubmed/29342121
http://dx.doi.org/10.3390/molecules23010197
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author Varinská, Lenka
Fáber, Lenka
Kello, Martin
Petrovová, Eva
Balážová, Ľudmila
Solár, Peter
Čoma, Matúš
Urdzík, Peter
Mojžiš, Ján
Švajdlenka, Emil
Mučaji, Pavel
Gál, Peter
author_facet Varinská, Lenka
Fáber, Lenka
Kello, Martin
Petrovová, Eva
Balážová, Ľudmila
Solár, Peter
Čoma, Matúš
Urdzík, Peter
Mojžiš, Ján
Švajdlenka, Emil
Mučaji, Pavel
Gál, Peter
author_sort Varinská, Lenka
collection PubMed
description In the present study we evaluated the anti-angiogenic activities of β-escin (the major active compound of Aesculus hippocastanum L. seeds). Human umbilical-vein endothelial cells (HUVECs) were used as an in vitro model for studying the molecular mechanism underlying the anti-angiogenic effect of β-escin. We investigated the in vitro effects on proliferation, migration, and tube formation of HUVECs and in vivo anti-angiogenic activity was evaluated in a chick chorioallantoic membrane (CAM) angiogenesis assay. Moreover, the effect on gene expressions was determined by the RT2 ProfilerTM human angiogenesis PCR Array. It was found that β-escin exerts inhibitory effect on the basic fibroblast growth factor (bFGF)-induced proliferation, migration and tube formation, as well as CAM angiogenesis in vivo. The inhibition of critical steps of angiogenic process observed with β-escin could be partially explained by suppression of Akt activation in response to bFGF. Moreover, the anti-angiogenic effects of β-escin could also be mediated via inhibition of EFNB2 and FGF-1 gene expressions in endothelial cells. In conclusion, β-escin affects endothelial cells as a negative mediator of angiogenesis in vitro and in vivo and may therefore be considered as a promising candidate for further research elucidating its underlying mechanism of action.
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spelling pubmed-60171402018-11-13 β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation Varinská, Lenka Fáber, Lenka Kello, Martin Petrovová, Eva Balážová, Ľudmila Solár, Peter Čoma, Matúš Urdzík, Peter Mojžiš, Ján Švajdlenka, Emil Mučaji, Pavel Gál, Peter Molecules Article In the present study we evaluated the anti-angiogenic activities of β-escin (the major active compound of Aesculus hippocastanum L. seeds). Human umbilical-vein endothelial cells (HUVECs) were used as an in vitro model for studying the molecular mechanism underlying the anti-angiogenic effect of β-escin. We investigated the in vitro effects on proliferation, migration, and tube formation of HUVECs and in vivo anti-angiogenic activity was evaluated in a chick chorioallantoic membrane (CAM) angiogenesis assay. Moreover, the effect on gene expressions was determined by the RT2 ProfilerTM human angiogenesis PCR Array. It was found that β-escin exerts inhibitory effect on the basic fibroblast growth factor (bFGF)-induced proliferation, migration and tube formation, as well as CAM angiogenesis in vivo. The inhibition of critical steps of angiogenic process observed with β-escin could be partially explained by suppression of Akt activation in response to bFGF. Moreover, the anti-angiogenic effects of β-escin could also be mediated via inhibition of EFNB2 and FGF-1 gene expressions in endothelial cells. In conclusion, β-escin affects endothelial cells as a negative mediator of angiogenesis in vitro and in vivo and may therefore be considered as a promising candidate for further research elucidating its underlying mechanism of action. MDPI 2018-01-17 /pmc/articles/PMC6017140/ /pubmed/29342121 http://dx.doi.org/10.3390/molecules23010197 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Varinská, Lenka
Fáber, Lenka
Kello, Martin
Petrovová, Eva
Balážová, Ľudmila
Solár, Peter
Čoma, Matúš
Urdzík, Peter
Mojžiš, Ján
Švajdlenka, Emil
Mučaji, Pavel
Gál, Peter
β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation
title β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation
title_full β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation
title_fullStr β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation
title_full_unstemmed β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation
title_short β-Escin Effectively Modulates HUVECs Proliferation and Tube Formation
title_sort β-escin effectively modulates huvecs proliferation and tube formation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017140/
https://www.ncbi.nlm.nih.gov/pubmed/29342121
http://dx.doi.org/10.3390/molecules23010197
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