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Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS

Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria, which are considered among the slowest growing organisms, with strong tolerance to adverse environmental conditions. There are about 400 genera and 1600 species of lichens and those belonging to the Usnea genus compris...

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Autores principales: Salgado, Francisco, Albornoz, Laura, Cortéz, Carmen, Stashenko, Elena, Urrea-Vallejo, Kelly, Nagles, Edgar, Galicia-Virviescas, Cesar, Cornejo, Alberto, Ardiles, Alejandro, Simirgiotis, Mario, García-Beltrán, Olimpo, Areche, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017147/
https://www.ncbi.nlm.nih.gov/pubmed/29280946
http://dx.doi.org/10.3390/molecules23010054
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author Salgado, Francisco
Albornoz, Laura
Cortéz, Carmen
Stashenko, Elena
Urrea-Vallejo, Kelly
Nagles, Edgar
Galicia-Virviescas, Cesar
Cornejo, Alberto
Ardiles, Alejandro
Simirgiotis, Mario
García-Beltrán, Olimpo
Areche, Carlos
author_facet Salgado, Francisco
Albornoz, Laura
Cortéz, Carmen
Stashenko, Elena
Urrea-Vallejo, Kelly
Nagles, Edgar
Galicia-Virviescas, Cesar
Cornejo, Alberto
Ardiles, Alejandro
Simirgiotis, Mario
García-Beltrán, Olimpo
Areche, Carlos
author_sort Salgado, Francisco
collection PubMed
description Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria, which are considered among the slowest growing organisms, with strong tolerance to adverse environmental conditions. There are about 400 genera and 1600 species of lichens and those belonging to the Usnea genus comprise about 360 of these species. Usnea lichens have been used since ancient times as dyes, cosmetics, preservatives, deodorants and folk medicines. The phytochemistry of the Usnea genus includes more than 60 compounds which belong to the following classes: depsides, depsidones, depsones, lactones, quinones, phenolics, polysaccharides, fatty acids and dibenzofurans. Due to scarce knowledge of metabolomic profiles of Usnea species (U. barbata, U. antarctica, U. rubicunda and U. subfloridana), a study based on UHPLC-ESI-OT-MS-MS was performed for a comprehensive characterization of their secondary metabolites. From the methanolic extracts of these species a total of 73 metabolites were identified for the first time using this hyphenated technique, including 34 compounds in U. barbata, 21 in U. antarctica, 38 in U. rubicunda and 37 in U. subfloridana. Besides, a total of 13 metabolites were not identified and reported so far, and could be new according to our data analysis. This study showed that this hyphenated technique is rapid, effective and accurate for phytochemical identification of lichen metabolites and the data collected could be useful for chemotaxonomic studies.
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spelling pubmed-60171472018-11-13 Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS Salgado, Francisco Albornoz, Laura Cortéz, Carmen Stashenko, Elena Urrea-Vallejo, Kelly Nagles, Edgar Galicia-Virviescas, Cesar Cornejo, Alberto Ardiles, Alejandro Simirgiotis, Mario García-Beltrán, Olimpo Areche, Carlos Molecules Article Lichens are symbiotic associations of fungi with microalgae and/or cyanobacteria, which are considered among the slowest growing organisms, with strong tolerance to adverse environmental conditions. There are about 400 genera and 1600 species of lichens and those belonging to the Usnea genus comprise about 360 of these species. Usnea lichens have been used since ancient times as dyes, cosmetics, preservatives, deodorants and folk medicines. The phytochemistry of the Usnea genus includes more than 60 compounds which belong to the following classes: depsides, depsidones, depsones, lactones, quinones, phenolics, polysaccharides, fatty acids and dibenzofurans. Due to scarce knowledge of metabolomic profiles of Usnea species (U. barbata, U. antarctica, U. rubicunda and U. subfloridana), a study based on UHPLC-ESI-OT-MS-MS was performed for a comprehensive characterization of their secondary metabolites. From the methanolic extracts of these species a total of 73 metabolites were identified for the first time using this hyphenated technique, including 34 compounds in U. barbata, 21 in U. antarctica, 38 in U. rubicunda and 37 in U. subfloridana. Besides, a total of 13 metabolites were not identified and reported so far, and could be new according to our data analysis. This study showed that this hyphenated technique is rapid, effective and accurate for phytochemical identification of lichen metabolites and the data collected could be useful for chemotaxonomic studies. MDPI 2017-12-27 /pmc/articles/PMC6017147/ /pubmed/29280946 http://dx.doi.org/10.3390/molecules23010054 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salgado, Francisco
Albornoz, Laura
Cortéz, Carmen
Stashenko, Elena
Urrea-Vallejo, Kelly
Nagles, Edgar
Galicia-Virviescas, Cesar
Cornejo, Alberto
Ardiles, Alejandro
Simirgiotis, Mario
García-Beltrán, Olimpo
Areche, Carlos
Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS
title Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS
title_full Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS
title_fullStr Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS
title_full_unstemmed Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS
title_short Secondary Metabolite Profiling of Species of the Genus Usnea by UHPLC-ESI-OT-MS-MS
title_sort secondary metabolite profiling of species of the genus usnea by uhplc-esi-ot-ms-ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017147/
https://www.ncbi.nlm.nih.gov/pubmed/29280946
http://dx.doi.org/10.3390/molecules23010054
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