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Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs

Exosomes are small vesicles which are produced by the cells and released into the surrounding space. They can transfer biomolecules into recipient cells. The main goal of the work was to study the exosome involvement in the cell transfer of hormonal resistance. The experiments were performed on in v...

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Autores principales: Semina, Svetlana E., Scherbakov, Alexander M., Vnukova, Anna A., Bagrov, Dmitry V., Evtushenko, Evgeniy G., Safronova, Vera M., Golovina, Daria A., Lyubchenko, Ludmila N., Gudkova, Margarita V., Krasil’nikov, Mikhail A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017149/
https://www.ncbi.nlm.nih.gov/pubmed/29617321
http://dx.doi.org/10.3390/molecules23040829
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author Semina, Svetlana E.
Scherbakov, Alexander M.
Vnukova, Anna A.
Bagrov, Dmitry V.
Evtushenko, Evgeniy G.
Safronova, Vera M.
Golovina, Daria A.
Lyubchenko, Ludmila N.
Gudkova, Margarita V.
Krasil’nikov, Mikhail A.
author_facet Semina, Svetlana E.
Scherbakov, Alexander M.
Vnukova, Anna A.
Bagrov, Dmitry V.
Evtushenko, Evgeniy G.
Safronova, Vera M.
Golovina, Daria A.
Lyubchenko, Ludmila N.
Gudkova, Margarita V.
Krasil’nikov, Mikhail A.
author_sort Semina, Svetlana E.
collection PubMed
description Exosomes are small vesicles which are produced by the cells and released into the surrounding space. They can transfer biomolecules into recipient cells. The main goal of the work was to study the exosome involvement in the cell transfer of hormonal resistance. The experiments were performed on in vitro cultured estrogen-dependent MCF-7 breast cancer cells and MCF-7 sublines resistant to SERM tamoxifen and/or biguanide metformin, which exerts its anti-proliferative effect, at least in a part, via the suppression of estrogen machinery. The exosomes were purified by differential ultracentrifugation, cell response to tamoxifen was determined by MTT test, and the level and activity of signaling proteins were determined by Western blot and reporter analysis. We found that the treatment of the parent MCF-7 cells with exosomes from the resistant cells within 14 days lead to the partial resistance of the MCF-7 cells to antiestrogen drugs. The primary resistant cells and the cells with the exosome-induced resistance were characterized with these common features: decrease in ERα activity and parallel activation of Akt and AP-1, NF-κB, and SNAIL1 transcriptional factors. In general, we evaluate the established results as the evidence of the possible exosome involvement in the transferring of the hormone/metformin resistance in breast cancer cells.
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spelling pubmed-60171492018-11-13 Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs Semina, Svetlana E. Scherbakov, Alexander M. Vnukova, Anna A. Bagrov, Dmitry V. Evtushenko, Evgeniy G. Safronova, Vera M. Golovina, Daria A. Lyubchenko, Ludmila N. Gudkova, Margarita V. Krasil’nikov, Mikhail A. Molecules Article Exosomes are small vesicles which are produced by the cells and released into the surrounding space. They can transfer biomolecules into recipient cells. The main goal of the work was to study the exosome involvement in the cell transfer of hormonal resistance. The experiments were performed on in vitro cultured estrogen-dependent MCF-7 breast cancer cells and MCF-7 sublines resistant to SERM tamoxifen and/or biguanide metformin, which exerts its anti-proliferative effect, at least in a part, via the suppression of estrogen machinery. The exosomes were purified by differential ultracentrifugation, cell response to tamoxifen was determined by MTT test, and the level and activity of signaling proteins were determined by Western blot and reporter analysis. We found that the treatment of the parent MCF-7 cells with exosomes from the resistant cells within 14 days lead to the partial resistance of the MCF-7 cells to antiestrogen drugs. The primary resistant cells and the cells with the exosome-induced resistance were characterized with these common features: decrease in ERα activity and parallel activation of Akt and AP-1, NF-κB, and SNAIL1 transcriptional factors. In general, we evaluate the established results as the evidence of the possible exosome involvement in the transferring of the hormone/metformin resistance in breast cancer cells. MDPI 2018-04-04 /pmc/articles/PMC6017149/ /pubmed/29617321 http://dx.doi.org/10.3390/molecules23040829 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Semina, Svetlana E.
Scherbakov, Alexander M.
Vnukova, Anna A.
Bagrov, Dmitry V.
Evtushenko, Evgeniy G.
Safronova, Vera M.
Golovina, Daria A.
Lyubchenko, Ludmila N.
Gudkova, Margarita V.
Krasil’nikov, Mikhail A.
Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs
title Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs
title_full Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs
title_fullStr Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs
title_full_unstemmed Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs
title_short Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs
title_sort exosome-mediated transfer of cancer cell resistance to antiestrogen drugs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017149/
https://www.ncbi.nlm.nih.gov/pubmed/29617321
http://dx.doi.org/10.3390/molecules23040829
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