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Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs
Exosomes are small vesicles which are produced by the cells and released into the surrounding space. They can transfer biomolecules into recipient cells. The main goal of the work was to study the exosome involvement in the cell transfer of hormonal resistance. The experiments were performed on in v...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017149/ https://www.ncbi.nlm.nih.gov/pubmed/29617321 http://dx.doi.org/10.3390/molecules23040829 |
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author | Semina, Svetlana E. Scherbakov, Alexander M. Vnukova, Anna A. Bagrov, Dmitry V. Evtushenko, Evgeniy G. Safronova, Vera M. Golovina, Daria A. Lyubchenko, Ludmila N. Gudkova, Margarita V. Krasil’nikov, Mikhail A. |
author_facet | Semina, Svetlana E. Scherbakov, Alexander M. Vnukova, Anna A. Bagrov, Dmitry V. Evtushenko, Evgeniy G. Safronova, Vera M. Golovina, Daria A. Lyubchenko, Ludmila N. Gudkova, Margarita V. Krasil’nikov, Mikhail A. |
author_sort | Semina, Svetlana E. |
collection | PubMed |
description | Exosomes are small vesicles which are produced by the cells and released into the surrounding space. They can transfer biomolecules into recipient cells. The main goal of the work was to study the exosome involvement in the cell transfer of hormonal resistance. The experiments were performed on in vitro cultured estrogen-dependent MCF-7 breast cancer cells and MCF-7 sublines resistant to SERM tamoxifen and/or biguanide metformin, which exerts its anti-proliferative effect, at least in a part, via the suppression of estrogen machinery. The exosomes were purified by differential ultracentrifugation, cell response to tamoxifen was determined by MTT test, and the level and activity of signaling proteins were determined by Western blot and reporter analysis. We found that the treatment of the parent MCF-7 cells with exosomes from the resistant cells within 14 days lead to the partial resistance of the MCF-7 cells to antiestrogen drugs. The primary resistant cells and the cells with the exosome-induced resistance were characterized with these common features: decrease in ERα activity and parallel activation of Akt and AP-1, NF-κB, and SNAIL1 transcriptional factors. In general, we evaluate the established results as the evidence of the possible exosome involvement in the transferring of the hormone/metformin resistance in breast cancer cells. |
format | Online Article Text |
id | pubmed-6017149 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60171492018-11-13 Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs Semina, Svetlana E. Scherbakov, Alexander M. Vnukova, Anna A. Bagrov, Dmitry V. Evtushenko, Evgeniy G. Safronova, Vera M. Golovina, Daria A. Lyubchenko, Ludmila N. Gudkova, Margarita V. Krasil’nikov, Mikhail A. Molecules Article Exosomes are small vesicles which are produced by the cells and released into the surrounding space. They can transfer biomolecules into recipient cells. The main goal of the work was to study the exosome involvement in the cell transfer of hormonal resistance. The experiments were performed on in vitro cultured estrogen-dependent MCF-7 breast cancer cells and MCF-7 sublines resistant to SERM tamoxifen and/or biguanide metformin, which exerts its anti-proliferative effect, at least in a part, via the suppression of estrogen machinery. The exosomes were purified by differential ultracentrifugation, cell response to tamoxifen was determined by MTT test, and the level and activity of signaling proteins were determined by Western blot and reporter analysis. We found that the treatment of the parent MCF-7 cells with exosomes from the resistant cells within 14 days lead to the partial resistance of the MCF-7 cells to antiestrogen drugs. The primary resistant cells and the cells with the exosome-induced resistance were characterized with these common features: decrease in ERα activity and parallel activation of Akt and AP-1, NF-κB, and SNAIL1 transcriptional factors. In general, we evaluate the established results as the evidence of the possible exosome involvement in the transferring of the hormone/metformin resistance in breast cancer cells. MDPI 2018-04-04 /pmc/articles/PMC6017149/ /pubmed/29617321 http://dx.doi.org/10.3390/molecules23040829 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Semina, Svetlana E. Scherbakov, Alexander M. Vnukova, Anna A. Bagrov, Dmitry V. Evtushenko, Evgeniy G. Safronova, Vera M. Golovina, Daria A. Lyubchenko, Ludmila N. Gudkova, Margarita V. Krasil’nikov, Mikhail A. Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs |
title | Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs |
title_full | Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs |
title_fullStr | Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs |
title_full_unstemmed | Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs |
title_short | Exosome-Mediated Transfer of Cancer Cell Resistance to Antiestrogen Drugs |
title_sort | exosome-mediated transfer of cancer cell resistance to antiestrogen drugs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017149/ https://www.ncbi.nlm.nih.gov/pubmed/29617321 http://dx.doi.org/10.3390/molecules23040829 |
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