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Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland

The antimicrobial activity of 144 samples of honeys including 95 products from apiaries located in Northern Poland was evaluated. The antibacterial activity of those natural products, their thermal stability, and activity in the presence of catalase was investigated by microdilution assays in titrat...

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Autores principales: Grecka, Katarzyna, Kuś, Piotr M., Worobo, Randy W., Szweda, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017226/
https://www.ncbi.nlm.nih.gov/pubmed/29382105
http://dx.doi.org/10.3390/molecules23020260
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author Grecka, Katarzyna
Kuś, Piotr M.
Worobo, Randy W.
Szweda, Piotr
author_facet Grecka, Katarzyna
Kuś, Piotr M.
Worobo, Randy W.
Szweda, Piotr
author_sort Grecka, Katarzyna
collection PubMed
description The antimicrobial activity of 144 samples of honeys including 95 products from apiaries located in Northern Poland was evaluated. The antibacterial activity of those natural products, their thermal stability, and activity in the presence of catalase was investigated by microdilution assays in titration plates. The MTT assay was performed for the determination of anti-biofilm activity. Spectrophotometric assays were used for the determination of antioxidant potential, total phenolic content, and ability to generate hydrogen peroxide. Some of the investigated honeys exhibited surprisingly high antimicrobial, especially anti-staphylococcal, potential, with Minimal Inhibitory Concentration (MIC) values of only 1.56% (v/v). Much higher resistance was observed in the case of staphylococci growing as biofilms. Lower concentrations of the product, up to 12.5% (v/v) stimulated its growth and effective eradication of biofilm required concentration of at least 25% (v/v). Hydrogen peroxide has been identified as a crucial contributor to the antimicrobial activity of honeys supplied by Polish beekeepers. However, some of the results suggest that phytochemicals, especially polyphenols, play an important role depending on botanical source (both positive, e.g., in the case of buckwheat honeys as well as negative, e.g., in the case of some rapeseed honeys) in their antimicrobial potential.
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spelling pubmed-60172262018-11-13 Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland Grecka, Katarzyna Kuś, Piotr M. Worobo, Randy W. Szweda, Piotr Molecules Article The antimicrobial activity of 144 samples of honeys including 95 products from apiaries located in Northern Poland was evaluated. The antibacterial activity of those natural products, their thermal stability, and activity in the presence of catalase was investigated by microdilution assays in titration plates. The MTT assay was performed for the determination of anti-biofilm activity. Spectrophotometric assays were used for the determination of antioxidant potential, total phenolic content, and ability to generate hydrogen peroxide. Some of the investigated honeys exhibited surprisingly high antimicrobial, especially anti-staphylococcal, potential, with Minimal Inhibitory Concentration (MIC) values of only 1.56% (v/v). Much higher resistance was observed in the case of staphylococci growing as biofilms. Lower concentrations of the product, up to 12.5% (v/v) stimulated its growth and effective eradication of biofilm required concentration of at least 25% (v/v). Hydrogen peroxide has been identified as a crucial contributor to the antimicrobial activity of honeys supplied by Polish beekeepers. However, some of the results suggest that phytochemicals, especially polyphenols, play an important role depending on botanical source (both positive, e.g., in the case of buckwheat honeys as well as negative, e.g., in the case of some rapeseed honeys) in their antimicrobial potential. MDPI 2018-01-28 /pmc/articles/PMC6017226/ /pubmed/29382105 http://dx.doi.org/10.3390/molecules23020260 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grecka, Katarzyna
Kuś, Piotr M.
Worobo, Randy W.
Szweda, Piotr
Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland
title Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland
title_full Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland
title_fullStr Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland
title_full_unstemmed Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland
title_short Study of the Anti-Staphylococcal Potential of Honeys Produced in Northern Poland
title_sort study of the anti-staphylococcal potential of honeys produced in northern poland
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017226/
https://www.ncbi.nlm.nih.gov/pubmed/29382105
http://dx.doi.org/10.3390/molecules23020260
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