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Reparative Effects of Astaxanthin-Hyaluronan Nanoaggregates against Retrorsine-CCl(4)-Induced Liver Fibrosis and Necrosis

Astaxanthin (Asta), a xanthophyll carotenoid, has been reported to be a strong antioxidative agent and has anti-inflammatory, antitumor and free radical-scavenging activities. However, inadequate stability and water solubility results in its low bioavailability. This study incorporated Asta into hyd...

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Autores principales: Wu, Yi Jhen, Wu, Yu Chiuan, Chen, I-Fen, Wu, Yi-Lung, Chuang, Chin Wen, Huang, Han Hsiang, Kuo, Shyh Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017246/
https://www.ncbi.nlm.nih.gov/pubmed/29565318
http://dx.doi.org/10.3390/molecules23040726
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author Wu, Yi Jhen
Wu, Yu Chiuan
Chen, I-Fen
Wu, Yi-Lung
Chuang, Chin Wen
Huang, Han Hsiang
Kuo, Shyh Ming
author_facet Wu, Yi Jhen
Wu, Yu Chiuan
Chen, I-Fen
Wu, Yi-Lung
Chuang, Chin Wen
Huang, Han Hsiang
Kuo, Shyh Ming
author_sort Wu, Yi Jhen
collection PubMed
description Astaxanthin (Asta), a xanthophyll carotenoid, has been reported to be a strong antioxidative agent and has anti-inflammatory, antitumor and free radical-scavenging activities. However, inadequate stability and water solubility results in its low bioavailability. This study incorporated Asta into hydrophilic hyaluronan nanoparticles (HAn) to produce Asta-HAn aggregates (AHAna) using an electrostatic field system and investigated the restorative effects of AHAna on retrorsine-CCl(4)-induced liver fibrosis in rats in vivo. Transmission electron microscopy (TEM) revealed that the prepared HAn were approximately 15 ± 2.1 nm in diameter and after the incorporation of Asta into HAn, the size increased to 210–500 nm. The incorporation efficiency of Asta was approximately 93% and approximately 54% of Asta was released after incubation for 18 h. Significant reductions in alanine aminotransferase and aspartate aminotransferase levels were observed after the rats were intraperitoneally injected with AHAna. Histopathological findings revealed the greatest reduction in hepatic fibrosis and hepatocyte necrosis in the rats after 2 weeks of intraperitoneal injection with AHAna, which is consistent with the data acquired from serum biochemical analysis. The restorative effects on liver damage displayed by AHAna in vivo demonstrated that Asta aggregated through HAn incorporation exerts therapeutic effects on liver fibrosis and necrosis.
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spelling pubmed-60172462018-11-13 Reparative Effects of Astaxanthin-Hyaluronan Nanoaggregates against Retrorsine-CCl(4)-Induced Liver Fibrosis and Necrosis Wu, Yi Jhen Wu, Yu Chiuan Chen, I-Fen Wu, Yi-Lung Chuang, Chin Wen Huang, Han Hsiang Kuo, Shyh Ming Molecules Article Astaxanthin (Asta), a xanthophyll carotenoid, has been reported to be a strong antioxidative agent and has anti-inflammatory, antitumor and free radical-scavenging activities. However, inadequate stability and water solubility results in its low bioavailability. This study incorporated Asta into hydrophilic hyaluronan nanoparticles (HAn) to produce Asta-HAn aggregates (AHAna) using an electrostatic field system and investigated the restorative effects of AHAna on retrorsine-CCl(4)-induced liver fibrosis in rats in vivo. Transmission electron microscopy (TEM) revealed that the prepared HAn were approximately 15 ± 2.1 nm in diameter and after the incorporation of Asta into HAn, the size increased to 210–500 nm. The incorporation efficiency of Asta was approximately 93% and approximately 54% of Asta was released after incubation for 18 h. Significant reductions in alanine aminotransferase and aspartate aminotransferase levels were observed after the rats were intraperitoneally injected with AHAna. Histopathological findings revealed the greatest reduction in hepatic fibrosis and hepatocyte necrosis in the rats after 2 weeks of intraperitoneal injection with AHAna, which is consistent with the data acquired from serum biochemical analysis. The restorative effects on liver damage displayed by AHAna in vivo demonstrated that Asta aggregated through HAn incorporation exerts therapeutic effects on liver fibrosis and necrosis. MDPI 2018-03-22 /pmc/articles/PMC6017246/ /pubmed/29565318 http://dx.doi.org/10.3390/molecules23040726 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Yi Jhen
Wu, Yu Chiuan
Chen, I-Fen
Wu, Yi-Lung
Chuang, Chin Wen
Huang, Han Hsiang
Kuo, Shyh Ming
Reparative Effects of Astaxanthin-Hyaluronan Nanoaggregates against Retrorsine-CCl(4)-Induced Liver Fibrosis and Necrosis
title Reparative Effects of Astaxanthin-Hyaluronan Nanoaggregates against Retrorsine-CCl(4)-Induced Liver Fibrosis and Necrosis
title_full Reparative Effects of Astaxanthin-Hyaluronan Nanoaggregates against Retrorsine-CCl(4)-Induced Liver Fibrosis and Necrosis
title_fullStr Reparative Effects of Astaxanthin-Hyaluronan Nanoaggregates against Retrorsine-CCl(4)-Induced Liver Fibrosis and Necrosis
title_full_unstemmed Reparative Effects of Astaxanthin-Hyaluronan Nanoaggregates against Retrorsine-CCl(4)-Induced Liver Fibrosis and Necrosis
title_short Reparative Effects of Astaxanthin-Hyaluronan Nanoaggregates against Retrorsine-CCl(4)-Induced Liver Fibrosis and Necrosis
title_sort reparative effects of astaxanthin-hyaluronan nanoaggregates against retrorsine-ccl(4)-induced liver fibrosis and necrosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017246/
https://www.ncbi.nlm.nih.gov/pubmed/29565318
http://dx.doi.org/10.3390/molecules23040726
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