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Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering
Chemical modification of gelatin using epigallocatechin gallate (EGCG) promotes bone formation in vivo. However, further improvements are required to increase the mechanical strength and bone-forming ability of fabricated EGCG-modified gelatin sponges (EGCG-GS) for practical applications in regenera...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017288/ https://www.ncbi.nlm.nih.gov/pubmed/29641458 http://dx.doi.org/10.3390/molecules23040876 |
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author | Honda, Yoshitomo Takeda, Yoshihiro Li, Peiqi Huang, Anqi Sasayama, Satoshi Hara, Eiki Uemura, Naoya Ueda, Mamoru Hashimoto, Masanori Arita, Kenji Matsumoto, Naoyuki Hashimoto, Yoshiya Baba, Shunsuke Tanaka, Tomonari |
author_facet | Honda, Yoshitomo Takeda, Yoshihiro Li, Peiqi Huang, Anqi Sasayama, Satoshi Hara, Eiki Uemura, Naoya Ueda, Mamoru Hashimoto, Masanori Arita, Kenji Matsumoto, Naoyuki Hashimoto, Yoshiya Baba, Shunsuke Tanaka, Tomonari |
author_sort | Honda, Yoshitomo |
collection | PubMed |
description | Chemical modification of gelatin using epigallocatechin gallate (EGCG) promotes bone formation in vivo. However, further improvements are required to increase the mechanical strength and bone-forming ability of fabricated EGCG-modified gelatin sponges (EGCG-GS) for practical applications in regenerative therapy. In the present study, we investigated whether vacuum heating-induced dehydrothermal cross-linking of EGCG-GS enhances bone formation in critical-sized rat calvarial defects. The bone-forming ability of vacuum-heated EGCG-GS (vhEGCG-GS) and other sponges was evaluated by micro-computed tomography and histological staining. The degradation of sponges was assessed using protein assays, and cell morphology and proliferation were verified by scanning electron microscopy and immunostaining using osteoblastic UMR106 cells in vitro. Four weeks after the implantation of sponges, greater bone formation was detected for vhEGCG-GS than for EGCG-GS or vacuum-heated gelatin sponges (dehydrothermal cross-linked sponges without EGCG). In vitro experiments revealed that the relatively low degradability of vhEGCG-GS supports cell attachment, proliferation, and cell–cell communication on the matrix. These findings suggest that vacuum heating enhanced the bone forming ability of EGCG-GS, possibly via the dehydrothermal cross-linking of EGCG-GS, which provides a scaffold for cells, and by maintaining the pharmacological effect of EGCG. |
format | Online Article Text |
id | pubmed-6017288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60172882018-11-13 Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering Honda, Yoshitomo Takeda, Yoshihiro Li, Peiqi Huang, Anqi Sasayama, Satoshi Hara, Eiki Uemura, Naoya Ueda, Mamoru Hashimoto, Masanori Arita, Kenji Matsumoto, Naoyuki Hashimoto, Yoshiya Baba, Shunsuke Tanaka, Tomonari Molecules Article Chemical modification of gelatin using epigallocatechin gallate (EGCG) promotes bone formation in vivo. However, further improvements are required to increase the mechanical strength and bone-forming ability of fabricated EGCG-modified gelatin sponges (EGCG-GS) for practical applications in regenerative therapy. In the present study, we investigated whether vacuum heating-induced dehydrothermal cross-linking of EGCG-GS enhances bone formation in critical-sized rat calvarial defects. The bone-forming ability of vacuum-heated EGCG-GS (vhEGCG-GS) and other sponges was evaluated by micro-computed tomography and histological staining. The degradation of sponges was assessed using protein assays, and cell morphology and proliferation were verified by scanning electron microscopy and immunostaining using osteoblastic UMR106 cells in vitro. Four weeks after the implantation of sponges, greater bone formation was detected for vhEGCG-GS than for EGCG-GS or vacuum-heated gelatin sponges (dehydrothermal cross-linked sponges without EGCG). In vitro experiments revealed that the relatively low degradability of vhEGCG-GS supports cell attachment, proliferation, and cell–cell communication on the matrix. These findings suggest that vacuum heating enhanced the bone forming ability of EGCG-GS, possibly via the dehydrothermal cross-linking of EGCG-GS, which provides a scaffold for cells, and by maintaining the pharmacological effect of EGCG. MDPI 2018-04-11 /pmc/articles/PMC6017288/ /pubmed/29641458 http://dx.doi.org/10.3390/molecules23040876 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Honda, Yoshitomo Takeda, Yoshihiro Li, Peiqi Huang, Anqi Sasayama, Satoshi Hara, Eiki Uemura, Naoya Ueda, Mamoru Hashimoto, Masanori Arita, Kenji Matsumoto, Naoyuki Hashimoto, Yoshiya Baba, Shunsuke Tanaka, Tomonari Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering |
title | Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering |
title_full | Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering |
title_fullStr | Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering |
title_full_unstemmed | Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering |
title_short | Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering |
title_sort | epigallocatechin gallate-modified gelatin sponges treated by vacuum heating as a novel scaffold for bone tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017288/ https://www.ncbi.nlm.nih.gov/pubmed/29641458 http://dx.doi.org/10.3390/molecules23040876 |
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