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Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering

Chemical modification of gelatin using epigallocatechin gallate (EGCG) promotes bone formation in vivo. However, further improvements are required to increase the mechanical strength and bone-forming ability of fabricated EGCG-modified gelatin sponges (EGCG-GS) for practical applications in regenera...

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Autores principales: Honda, Yoshitomo, Takeda, Yoshihiro, Li, Peiqi, Huang, Anqi, Sasayama, Satoshi, Hara, Eiki, Uemura, Naoya, Ueda, Mamoru, Hashimoto, Masanori, Arita, Kenji, Matsumoto, Naoyuki, Hashimoto, Yoshiya, Baba, Shunsuke, Tanaka, Tomonari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017288/
https://www.ncbi.nlm.nih.gov/pubmed/29641458
http://dx.doi.org/10.3390/molecules23040876
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author Honda, Yoshitomo
Takeda, Yoshihiro
Li, Peiqi
Huang, Anqi
Sasayama, Satoshi
Hara, Eiki
Uemura, Naoya
Ueda, Mamoru
Hashimoto, Masanori
Arita, Kenji
Matsumoto, Naoyuki
Hashimoto, Yoshiya
Baba, Shunsuke
Tanaka, Tomonari
author_facet Honda, Yoshitomo
Takeda, Yoshihiro
Li, Peiqi
Huang, Anqi
Sasayama, Satoshi
Hara, Eiki
Uemura, Naoya
Ueda, Mamoru
Hashimoto, Masanori
Arita, Kenji
Matsumoto, Naoyuki
Hashimoto, Yoshiya
Baba, Shunsuke
Tanaka, Tomonari
author_sort Honda, Yoshitomo
collection PubMed
description Chemical modification of gelatin using epigallocatechin gallate (EGCG) promotes bone formation in vivo. However, further improvements are required to increase the mechanical strength and bone-forming ability of fabricated EGCG-modified gelatin sponges (EGCG-GS) for practical applications in regenerative therapy. In the present study, we investigated whether vacuum heating-induced dehydrothermal cross-linking of EGCG-GS enhances bone formation in critical-sized rat calvarial defects. The bone-forming ability of vacuum-heated EGCG-GS (vhEGCG-GS) and other sponges was evaluated by micro-computed tomography and histological staining. The degradation of sponges was assessed using protein assays, and cell morphology and proliferation were verified by scanning electron microscopy and immunostaining using osteoblastic UMR106 cells in vitro. Four weeks after the implantation of sponges, greater bone formation was detected for vhEGCG-GS than for EGCG-GS or vacuum-heated gelatin sponges (dehydrothermal cross-linked sponges without EGCG). In vitro experiments revealed that the relatively low degradability of vhEGCG-GS supports cell attachment, proliferation, and cell–cell communication on the matrix. These findings suggest that vacuum heating enhanced the bone forming ability of EGCG-GS, possibly via the dehydrothermal cross-linking of EGCG-GS, which provides a scaffold for cells, and by maintaining the pharmacological effect of EGCG.
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spelling pubmed-60172882018-11-13 Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering Honda, Yoshitomo Takeda, Yoshihiro Li, Peiqi Huang, Anqi Sasayama, Satoshi Hara, Eiki Uemura, Naoya Ueda, Mamoru Hashimoto, Masanori Arita, Kenji Matsumoto, Naoyuki Hashimoto, Yoshiya Baba, Shunsuke Tanaka, Tomonari Molecules Article Chemical modification of gelatin using epigallocatechin gallate (EGCG) promotes bone formation in vivo. However, further improvements are required to increase the mechanical strength and bone-forming ability of fabricated EGCG-modified gelatin sponges (EGCG-GS) for practical applications in regenerative therapy. In the present study, we investigated whether vacuum heating-induced dehydrothermal cross-linking of EGCG-GS enhances bone formation in critical-sized rat calvarial defects. The bone-forming ability of vacuum-heated EGCG-GS (vhEGCG-GS) and other sponges was evaluated by micro-computed tomography and histological staining. The degradation of sponges was assessed using protein assays, and cell morphology and proliferation were verified by scanning electron microscopy and immunostaining using osteoblastic UMR106 cells in vitro. Four weeks after the implantation of sponges, greater bone formation was detected for vhEGCG-GS than for EGCG-GS or vacuum-heated gelatin sponges (dehydrothermal cross-linked sponges without EGCG). In vitro experiments revealed that the relatively low degradability of vhEGCG-GS supports cell attachment, proliferation, and cell–cell communication on the matrix. These findings suggest that vacuum heating enhanced the bone forming ability of EGCG-GS, possibly via the dehydrothermal cross-linking of EGCG-GS, which provides a scaffold for cells, and by maintaining the pharmacological effect of EGCG. MDPI 2018-04-11 /pmc/articles/PMC6017288/ /pubmed/29641458 http://dx.doi.org/10.3390/molecules23040876 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Honda, Yoshitomo
Takeda, Yoshihiro
Li, Peiqi
Huang, Anqi
Sasayama, Satoshi
Hara, Eiki
Uemura, Naoya
Ueda, Mamoru
Hashimoto, Masanori
Arita, Kenji
Matsumoto, Naoyuki
Hashimoto, Yoshiya
Baba, Shunsuke
Tanaka, Tomonari
Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering
title Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering
title_full Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering
title_fullStr Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering
title_full_unstemmed Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering
title_short Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering
title_sort epigallocatechin gallate-modified gelatin sponges treated by vacuum heating as a novel scaffold for bone tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017288/
https://www.ncbi.nlm.nih.gov/pubmed/29641458
http://dx.doi.org/10.3390/molecules23040876
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