Cargando…

Identification, Characterization, and Expression Analysis of a FMRFamide-Like Peptide Gene in the Common Chinese Cuttlefish (Sepiella japonica)

The peptide FMRFamide is one of the well-known peptides involved in multiple physiological processes in the phylum Mollusca. In this study, a FMRFamide gene (GenBank accession No. KJ933411) was identified in a cuttlefish species called Sepiella japonica and was designated as SjFMRFamide. The total l...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Ying, Cao, Zihao, Li, Haifeng, Liu, Huihui, Lü, Zhenming, Chi, Changfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017766/
https://www.ncbi.nlm.nih.gov/pubmed/29570647
http://dx.doi.org/10.3390/molecules23040742
_version_ 1783334820573609984
author Li, Ying
Cao, Zihao
Li, Haifeng
Liu, Huihui
Lü, Zhenming
Chi, Changfeng
author_facet Li, Ying
Cao, Zihao
Li, Haifeng
Liu, Huihui
Lü, Zhenming
Chi, Changfeng
author_sort Li, Ying
collection PubMed
description The peptide FMRFamide is one of the well-known peptides involved in multiple physiological processes in the phylum Mollusca. In this study, a FMRFamide gene (GenBank accession No. KJ933411) was identified in a cuttlefish species called Sepiella japonica and was designated as SjFMRFamide. The total length of the SjFMRFamide sequence was found to be 1880 bp while the open reading frame contained 996 bp encoding a protein of 331 amino acid residues with a predicted isoelectric point (pI) and molecular weight (MW) of 9.18 and 38.8 kDa along with a 333 bp 5′-untranslated region (UTR) and 551 bp 3′-UTR. The deduced SjFMRFamide precursor protein contains one signal peptide and expresses four kinds FMRFamide-related peptides including a single copy of FLRFamide, ALSGDAFLRFamide, and FIRFamide and multiple copies of FMRFamide. Results of phylogenetic relation analysis strongly indicated that the sequence of this gene shares high identity with the genes of known FMRFamides. Spatial expression analysis indicated the highest mRNA expression of SjFMRFamide in the brain of male and female cuttlefishes among the eight tissues analyzed. An in situ hybridization assay of the brain indicated that SjFMRFamide was transcribed in several functional lobes, which suggests that it might be related to many physiological regulatory mechanisms. This is the first study describing FMRFamide in S. japonica and the results may contribute to future studies of neuropeptide evolution or may prove useful for the development of aquaculture methods for this cuttlefish species.
format Online
Article
Text
id pubmed-6017766
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-60177662018-11-13 Identification, Characterization, and Expression Analysis of a FMRFamide-Like Peptide Gene in the Common Chinese Cuttlefish (Sepiella japonica) Li, Ying Cao, Zihao Li, Haifeng Liu, Huihui Lü, Zhenming Chi, Changfeng Molecules Article The peptide FMRFamide is one of the well-known peptides involved in multiple physiological processes in the phylum Mollusca. In this study, a FMRFamide gene (GenBank accession No. KJ933411) was identified in a cuttlefish species called Sepiella japonica and was designated as SjFMRFamide. The total length of the SjFMRFamide sequence was found to be 1880 bp while the open reading frame contained 996 bp encoding a protein of 331 amino acid residues with a predicted isoelectric point (pI) and molecular weight (MW) of 9.18 and 38.8 kDa along with a 333 bp 5′-untranslated region (UTR) and 551 bp 3′-UTR. The deduced SjFMRFamide precursor protein contains one signal peptide and expresses four kinds FMRFamide-related peptides including a single copy of FLRFamide, ALSGDAFLRFamide, and FIRFamide and multiple copies of FMRFamide. Results of phylogenetic relation analysis strongly indicated that the sequence of this gene shares high identity with the genes of known FMRFamides. Spatial expression analysis indicated the highest mRNA expression of SjFMRFamide in the brain of male and female cuttlefishes among the eight tissues analyzed. An in situ hybridization assay of the brain indicated that SjFMRFamide was transcribed in several functional lobes, which suggests that it might be related to many physiological regulatory mechanisms. This is the first study describing FMRFamide in S. japonica and the results may contribute to future studies of neuropeptide evolution or may prove useful for the development of aquaculture methods for this cuttlefish species. MDPI 2018-03-23 /pmc/articles/PMC6017766/ /pubmed/29570647 http://dx.doi.org/10.3390/molecules23040742 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Ying
Cao, Zihao
Li, Haifeng
Liu, Huihui
Lü, Zhenming
Chi, Changfeng
Identification, Characterization, and Expression Analysis of a FMRFamide-Like Peptide Gene in the Common Chinese Cuttlefish (Sepiella japonica)
title Identification, Characterization, and Expression Analysis of a FMRFamide-Like Peptide Gene in the Common Chinese Cuttlefish (Sepiella japonica)
title_full Identification, Characterization, and Expression Analysis of a FMRFamide-Like Peptide Gene in the Common Chinese Cuttlefish (Sepiella japonica)
title_fullStr Identification, Characterization, and Expression Analysis of a FMRFamide-Like Peptide Gene in the Common Chinese Cuttlefish (Sepiella japonica)
title_full_unstemmed Identification, Characterization, and Expression Analysis of a FMRFamide-Like Peptide Gene in the Common Chinese Cuttlefish (Sepiella japonica)
title_short Identification, Characterization, and Expression Analysis of a FMRFamide-Like Peptide Gene in the Common Chinese Cuttlefish (Sepiella japonica)
title_sort identification, characterization, and expression analysis of a fmrfamide-like peptide gene in the common chinese cuttlefish (sepiella japonica)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017766/
https://www.ncbi.nlm.nih.gov/pubmed/29570647
http://dx.doi.org/10.3390/molecules23040742
work_keys_str_mv AT liying identificationcharacterizationandexpressionanalysisofafmrfamidelikepeptidegeneinthecommonchinesecuttlefishsepiellajaponica
AT caozihao identificationcharacterizationandexpressionanalysisofafmrfamidelikepeptidegeneinthecommonchinesecuttlefishsepiellajaponica
AT lihaifeng identificationcharacterizationandexpressionanalysisofafmrfamidelikepeptidegeneinthecommonchinesecuttlefishsepiellajaponica
AT liuhuihui identificationcharacterizationandexpressionanalysisofafmrfamidelikepeptidegeneinthecommonchinesecuttlefishsepiellajaponica
AT luzhenming identificationcharacterizationandexpressionanalysisofafmrfamidelikepeptidegeneinthecommonchinesecuttlefishsepiellajaponica
AT chichangfeng identificationcharacterizationandexpressionanalysisofafmrfamidelikepeptidegeneinthecommonchinesecuttlefishsepiellajaponica