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Recent Advances in Detecting Mitochondrial DNA Heteroplasmic Variations
The co-existence of wild-type and mutated mitochondrial DNA (mtDNA) molecules termed heteroplasmy becomes a research hot point of mitochondria. In this review, we listed several methods of mtDNA heteroplasmy research, including the enrichment of mtDNA and the way of calling heteroplasmic variations....
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017848/ https://www.ncbi.nlm.nih.gov/pubmed/29401641 http://dx.doi.org/10.3390/molecules23020323 |
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author | Duan, Mengqin Tu, Jing Lu, Zuhong |
author_facet | Duan, Mengqin Tu, Jing Lu, Zuhong |
author_sort | Duan, Mengqin |
collection | PubMed |
description | The co-existence of wild-type and mutated mitochondrial DNA (mtDNA) molecules termed heteroplasmy becomes a research hot point of mitochondria. In this review, we listed several methods of mtDNA heteroplasmy research, including the enrichment of mtDNA and the way of calling heteroplasmic variations. At the present, while calling the novel ultra-low level heteroplasmy, high-throughput sequencing method is dominant while the detection limit of recorded mutations is accurate to 0.01% using the other quantitative approaches. In the future, the studies of mtDNA heteroplasmy may pay more attention to the single-cell level and focus on the linkage of mutations. |
format | Online Article Text |
id | pubmed-6017848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60178482018-11-13 Recent Advances in Detecting Mitochondrial DNA Heteroplasmic Variations Duan, Mengqin Tu, Jing Lu, Zuhong Molecules Review The co-existence of wild-type and mutated mitochondrial DNA (mtDNA) molecules termed heteroplasmy becomes a research hot point of mitochondria. In this review, we listed several methods of mtDNA heteroplasmy research, including the enrichment of mtDNA and the way of calling heteroplasmic variations. At the present, while calling the novel ultra-low level heteroplasmy, high-throughput sequencing method is dominant while the detection limit of recorded mutations is accurate to 0.01% using the other quantitative approaches. In the future, the studies of mtDNA heteroplasmy may pay more attention to the single-cell level and focus on the linkage of mutations. MDPI 2018-02-03 /pmc/articles/PMC6017848/ /pubmed/29401641 http://dx.doi.org/10.3390/molecules23020323 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Duan, Mengqin Tu, Jing Lu, Zuhong Recent Advances in Detecting Mitochondrial DNA Heteroplasmic Variations |
title | Recent Advances in Detecting Mitochondrial DNA Heteroplasmic Variations |
title_full | Recent Advances in Detecting Mitochondrial DNA Heteroplasmic Variations |
title_fullStr | Recent Advances in Detecting Mitochondrial DNA Heteroplasmic Variations |
title_full_unstemmed | Recent Advances in Detecting Mitochondrial DNA Heteroplasmic Variations |
title_short | Recent Advances in Detecting Mitochondrial DNA Heteroplasmic Variations |
title_sort | recent advances in detecting mitochondrial dna heteroplasmic variations |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6017848/ https://www.ncbi.nlm.nih.gov/pubmed/29401641 http://dx.doi.org/10.3390/molecules23020323 |
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