Cargando…

Association between (GT)n Repeats in Heme Oxygenase-1 Gene Promoter and 3-Year Survival of Patients with Acute Leukemia: a Controlled, Cross-Sectional Study

Background: Acute leukemia is a common pediatric cancer. Novel strategies for treatment of acute leukemia have been developed, but treatment resistance is remained as the most problematic issue. It is hypothesized that the HO-1 gene up-regulation is responsible for tumor resistance to chemotherapy o...

Descripción completa

Detalles Bibliográficos
Autores principales: Kazemi, Mohammad, Khosravian, Farinaz, Sameti, Amir Abbas, Moafi, Alireza, Merasi, Mohammad Reza, Salehi, Mansour, Nejati, Majid, Behjati, Mohaddeseh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences, Hematology-Oncology and Stem Cell Transplantation Research Center 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018253/
https://www.ncbi.nlm.nih.gov/pubmed/29951178
_version_ 1783334916498391040
author Kazemi, Mohammad
Khosravian, Farinaz
Sameti, Amir Abbas
Moafi, Alireza
Merasi, Mohammad Reza
Salehi, Mansour
Nejati, Majid
Behjati, Mohaddeseh
author_facet Kazemi, Mohammad
Khosravian, Farinaz
Sameti, Amir Abbas
Moafi, Alireza
Merasi, Mohammad Reza
Salehi, Mansour
Nejati, Majid
Behjati, Mohaddeseh
author_sort Kazemi, Mohammad
collection PubMed
description Background: Acute leukemia is a common pediatric cancer. Novel strategies for treatment of acute leukemia have been developed, but treatment resistance is remained as the most problematic issue. It is hypothesized that the HO-1 gene up-regulation is responsible for tumor resistance to chemotherapy or radiotherapy-induced apoptosis. The levels of HO-1 expression are related to (GT)(n) microsatellite polymorphisms in the location of its promoter. This study designed to compare allelic frequencies of (GT)(n) microsatellite polymorphisms in HO-1 gene between acute leukemia patients and healthy controls. Indeed, 3-year disease-free survival was also evaluated. Methods: Sixty-three patients with acute leukemia and seventy healthy infants were included in this study. We used the medical records of patients to collect information about survival after chemotherapy. The number of GT repeats in HO-1 promoter was determined by an ABI 3100 sequencer. Results: The HO-1 GT repeats ranged from 14 to 34 with peaks at 27 repeats in both cases and controls. Children with longer alleles ((GT)(n )≥ 27) had enhanced 3-year survival rate after treatment with chemotherapy or radiotherapy (P<0.05). Conclusion: Although no significant differences were observed between leukemia patients and controls regarding allelic frequency, we found elevated frequency of “LL” genotype in leukemia patients with good prognosis and 3-year surveillance. Radiotherapy and chemotherapy might elevate the expression levels of HO-1 with subsequent increased resistance of leukemia patients to therapy.
format Online
Article
Text
id pubmed-6018253
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Tehran University of Medical Sciences, Hematology-Oncology and Stem Cell Transplantation Research Center
record_format MEDLINE/PubMed
spelling pubmed-60182532018-06-27 Association between (GT)n Repeats in Heme Oxygenase-1 Gene Promoter and 3-Year Survival of Patients with Acute Leukemia: a Controlled, Cross-Sectional Study Kazemi, Mohammad Khosravian, Farinaz Sameti, Amir Abbas Moafi, Alireza Merasi, Mohammad Reza Salehi, Mansour Nejati, Majid Behjati, Mohaddeseh Int J Hematol Oncol Stem Cell Res Original Article Background: Acute leukemia is a common pediatric cancer. Novel strategies for treatment of acute leukemia have been developed, but treatment resistance is remained as the most problematic issue. It is hypothesized that the HO-1 gene up-regulation is responsible for tumor resistance to chemotherapy or radiotherapy-induced apoptosis. The levels of HO-1 expression are related to (GT)(n) microsatellite polymorphisms in the location of its promoter. This study designed to compare allelic frequencies of (GT)(n) microsatellite polymorphisms in HO-1 gene between acute leukemia patients and healthy controls. Indeed, 3-year disease-free survival was also evaluated. Methods: Sixty-three patients with acute leukemia and seventy healthy infants were included in this study. We used the medical records of patients to collect information about survival after chemotherapy. The number of GT repeats in HO-1 promoter was determined by an ABI 3100 sequencer. Results: The HO-1 GT repeats ranged from 14 to 34 with peaks at 27 repeats in both cases and controls. Children with longer alleles ((GT)(n )≥ 27) had enhanced 3-year survival rate after treatment with chemotherapy or radiotherapy (P<0.05). Conclusion: Although no significant differences were observed between leukemia patients and controls regarding allelic frequency, we found elevated frequency of “LL” genotype in leukemia patients with good prognosis and 3-year surveillance. Radiotherapy and chemotherapy might elevate the expression levels of HO-1 with subsequent increased resistance of leukemia patients to therapy. Tehran University of Medical Sciences, Hematology-Oncology and Stem Cell Transplantation Research Center 2018-01-01 /pmc/articles/PMC6018253/ /pubmed/29951178 Text en Copyright : © International Journal of Hematology-Oncology and Stem Cell Research & Tehran University of Medical Sciences This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kazemi, Mohammad
Khosravian, Farinaz
Sameti, Amir Abbas
Moafi, Alireza
Merasi, Mohammad Reza
Salehi, Mansour
Nejati, Majid
Behjati, Mohaddeseh
Association between (GT)n Repeats in Heme Oxygenase-1 Gene Promoter and 3-Year Survival of Patients with Acute Leukemia: a Controlled, Cross-Sectional Study
title Association between (GT)n Repeats in Heme Oxygenase-1 Gene Promoter and 3-Year Survival of Patients with Acute Leukemia: a Controlled, Cross-Sectional Study
title_full Association between (GT)n Repeats in Heme Oxygenase-1 Gene Promoter and 3-Year Survival of Patients with Acute Leukemia: a Controlled, Cross-Sectional Study
title_fullStr Association between (GT)n Repeats in Heme Oxygenase-1 Gene Promoter and 3-Year Survival of Patients with Acute Leukemia: a Controlled, Cross-Sectional Study
title_full_unstemmed Association between (GT)n Repeats in Heme Oxygenase-1 Gene Promoter and 3-Year Survival of Patients with Acute Leukemia: a Controlled, Cross-Sectional Study
title_short Association between (GT)n Repeats in Heme Oxygenase-1 Gene Promoter and 3-Year Survival of Patients with Acute Leukemia: a Controlled, Cross-Sectional Study
title_sort association between (gt)n repeats in heme oxygenase-1 gene promoter and 3-year survival of patients with acute leukemia: a controlled, cross-sectional study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018253/
https://www.ncbi.nlm.nih.gov/pubmed/29951178
work_keys_str_mv AT kazemimohammad associationbetweengtnrepeatsinhemeoxygenase1genepromoterand3yearsurvivalofpatientswithacuteleukemiaacontrolledcrosssectionalstudy
AT khosravianfarinaz associationbetweengtnrepeatsinhemeoxygenase1genepromoterand3yearsurvivalofpatientswithacuteleukemiaacontrolledcrosssectionalstudy
AT sametiamirabbas associationbetweengtnrepeatsinhemeoxygenase1genepromoterand3yearsurvivalofpatientswithacuteleukemiaacontrolledcrosssectionalstudy
AT moafialireza associationbetweengtnrepeatsinhemeoxygenase1genepromoterand3yearsurvivalofpatientswithacuteleukemiaacontrolledcrosssectionalstudy
AT merasimohammadreza associationbetweengtnrepeatsinhemeoxygenase1genepromoterand3yearsurvivalofpatientswithacuteleukemiaacontrolledcrosssectionalstudy
AT salehimansour associationbetweengtnrepeatsinhemeoxygenase1genepromoterand3yearsurvivalofpatientswithacuteleukemiaacontrolledcrosssectionalstudy
AT nejatimajid associationbetweengtnrepeatsinhemeoxygenase1genepromoterand3yearsurvivalofpatientswithacuteleukemiaacontrolledcrosssectionalstudy
AT behjatimohaddeseh associationbetweengtnrepeatsinhemeoxygenase1genepromoterand3yearsurvivalofpatientswithacuteleukemiaacontrolledcrosssectionalstudy