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Fas Ligand Gene (Faslg) Plays an Important Role in Nerve Degeneration and Regeneration After Rat Sciatic Nerve Injury

Wallerian degeneration (WD) is associated with changes in the expression levels of a large number of genes. However, the effects of these up- or down-regulated genes are poorly understood. We have reported some key factors that are differentially regulated during WD in our previous research. Here, w...

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Autores principales: Li, Yuting, Sun, Yuhua, Cai, Min, Zhang, Huanhuan, Gao, Nannan, Huang, Huiwei, Cui, Shusen, Yao, Dengbing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018423/
https://www.ncbi.nlm.nih.gov/pubmed/29970988
http://dx.doi.org/10.3389/fnmol.2018.00210
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author Li, Yuting
Sun, Yuhua
Cai, Min
Zhang, Huanhuan
Gao, Nannan
Huang, Huiwei
Cui, Shusen
Yao, Dengbing
author_facet Li, Yuting
Sun, Yuhua
Cai, Min
Zhang, Huanhuan
Gao, Nannan
Huang, Huiwei
Cui, Shusen
Yao, Dengbing
author_sort Li, Yuting
collection PubMed
description Wallerian degeneration (WD) is associated with changes in the expression levels of a large number of genes. However, the effects of these up- or down-regulated genes are poorly understood. We have reported some key factors that are differentially regulated during WD in our previous research. Here, we explored the roles of Fas ligand gene (Faslg) in WD after rat sciatic nerve injury. The data showed that Faslg was up-regulated in injured nerves. Expression changed of Faslg in Schwann cells (SCs) resulted in alterations in the release of related factors. Silencing or overexpression of Faslg affected SC proliferation, migration, and apoptosis through β-catenin, nuclear factor-κB (NF-κB), and caspase-3 pathways in vivo and in vitro. Our data suggest that Faslg is a key regulatory gene that affects nerve repair and regeneration in peripheral nerve injury. This study sheds new light on the effects of Faslg on peripheral nerve degeneration and/or regeneration.
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spelling pubmed-60184232018-07-03 Fas Ligand Gene (Faslg) Plays an Important Role in Nerve Degeneration and Regeneration After Rat Sciatic Nerve Injury Li, Yuting Sun, Yuhua Cai, Min Zhang, Huanhuan Gao, Nannan Huang, Huiwei Cui, Shusen Yao, Dengbing Front Mol Neurosci Neuroscience Wallerian degeneration (WD) is associated with changes in the expression levels of a large number of genes. However, the effects of these up- or down-regulated genes are poorly understood. We have reported some key factors that are differentially regulated during WD in our previous research. Here, we explored the roles of Fas ligand gene (Faslg) in WD after rat sciatic nerve injury. The data showed that Faslg was up-regulated in injured nerves. Expression changed of Faslg in Schwann cells (SCs) resulted in alterations in the release of related factors. Silencing or overexpression of Faslg affected SC proliferation, migration, and apoptosis through β-catenin, nuclear factor-κB (NF-κB), and caspase-3 pathways in vivo and in vitro. Our data suggest that Faslg is a key regulatory gene that affects nerve repair and regeneration in peripheral nerve injury. This study sheds new light on the effects of Faslg on peripheral nerve degeneration and/or regeneration. Frontiers Media S.A. 2018-06-19 /pmc/articles/PMC6018423/ /pubmed/29970988 http://dx.doi.org/10.3389/fnmol.2018.00210 Text en Copyright © 2018 Li, Sun, Cai, Zhang, Gao, Huang, Cui and Yao. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Li, Yuting
Sun, Yuhua
Cai, Min
Zhang, Huanhuan
Gao, Nannan
Huang, Huiwei
Cui, Shusen
Yao, Dengbing
Fas Ligand Gene (Faslg) Plays an Important Role in Nerve Degeneration and Regeneration After Rat Sciatic Nerve Injury
title Fas Ligand Gene (Faslg) Plays an Important Role in Nerve Degeneration and Regeneration After Rat Sciatic Nerve Injury
title_full Fas Ligand Gene (Faslg) Plays an Important Role in Nerve Degeneration and Regeneration After Rat Sciatic Nerve Injury
title_fullStr Fas Ligand Gene (Faslg) Plays an Important Role in Nerve Degeneration and Regeneration After Rat Sciatic Nerve Injury
title_full_unstemmed Fas Ligand Gene (Faslg) Plays an Important Role in Nerve Degeneration and Regeneration After Rat Sciatic Nerve Injury
title_short Fas Ligand Gene (Faslg) Plays an Important Role in Nerve Degeneration and Regeneration After Rat Sciatic Nerve Injury
title_sort fas ligand gene (faslg) plays an important role in nerve degeneration and regeneration after rat sciatic nerve injury
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018423/
https://www.ncbi.nlm.nih.gov/pubmed/29970988
http://dx.doi.org/10.3389/fnmol.2018.00210
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