Exploring the Potential Roles of Band 3 and Aquaporin-1 in Blood CO(2) Transport–Inspired by Comparative Studies of Glycophorin B-A-B Hybrid Protein GP.Mur

The Cl(—)/HCO(3)(—) exchanger band 3 is functionally relevant to blood CO(2) transport. Band 3 is the most abundant membrane protein in human red blood cells (RBCs). Our understanding of its physiological functions mainly came from clinical cases associated with band 3 mutations. Severe reduction in band 3 expression affects blood HCO(3)(—)/CO(2) metabolism. What could happen physiologically if band 3 expression is elevated instead? In some areas of Southeast Asia, about 1–10% of the populations express GP.Mur, a glycophorin B-A-B hybrid membrane protein important in the field of transfusion medicine. GP.Mur functions to promote band 3 expression, and GP.Mur red cells can be deemed as a naturally occurred model for higher band 3 expression. This review first compares the functional consequences of band 3 at different levels, and suggests a critical role of band 3 in postnatal CO(2) respiration. The second part of the review explores the transport of water, which is the other substrate for intra-erythrocytic CO(2)/HCO(3)(—) conversion (an essential step in blood CO(2) transport). Despite that water is considered unlimited physiologically, it is unclear whether water channel aquaporin-1 (AQP1) abundantly expressed in RBCs is functionally involved in CO(2) transport. Research in this area is complicated by the fact that the H(2)O/CO(2)-transporting function of AQP1 is replaceable by other erythrocyte channels/transporters (e.g., UT-B/GLUT1 for H(2)O; RhAG for CO(2)). Recently, using carbonic anhydrase II (CAII)-filled erythrocyte vesicles, AQP1 has been demonstrated to transport water for the CAII-mediated reaction, CO(2(g)) + H(2)O ⇌ HCO(3)(—)((aq)) + H(+)((aq)). AQP1 is structurally associated with some population of band 3 complexes on the erythrocyte membrane in an osmotically responsive fashion. The current findings reveal transient interaction among components within the band 3-central, CO(2)-transport metabolon (AQP1, band 3, CAII and deoxygenated hemoglobin). Their dynamic interaction is envisioned to facilitate blood CO(2) respiration, in the presence of constantly changing osmotic and hemodynamic stresses during circulation.