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High content screen for identifying small-molecule LC3B-localization modulators in a renal cancer cell line
Forms of selective autophagy have now been recognized to regulate flux in many intracellular processes. Specific pathways and functions have been identified for mitophagy, ERphagy, and other selective autophagies; yet there is no consensus in whether and how autophagy regulates protein maintenance i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018519/ https://www.ncbi.nlm.nih.gov/pubmed/29944143 http://dx.doi.org/10.1038/sdata.2018.116 |
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author | Kolla, Likhitha Heo, David S. Rosenberg, Daniel P. Barlow, Sara A. Maximova, Anna A. Cassio, Emily E. Buchser, William J. |
author_facet | Kolla, Likhitha Heo, David S. Rosenberg, Daniel P. Barlow, Sara A. Maximova, Anna A. Cassio, Emily E. Buchser, William J. |
author_sort | Kolla, Likhitha |
collection | PubMed |
description | Forms of selective autophagy have now been recognized to regulate flux in many intracellular processes. Specific pathways and functions have been identified for mitophagy, ERphagy, and other selective autophagies; yet there is no consensus in whether and how autophagy regulates protein maintenance in and around the nucleus. Such processes are of interest for potential degradation of DNA and nuclear envelope proteins in various disease states. The mechanistic details of such nucleus-related autophagic processes remain elusive due to the lack of chemical or genetic regulators to manipulate and follow the process in vitro. Here, we describe a high content screen from which we identified small chemical compounds that can modulate the localization of the autophagy marker MAP1LC3B (LC3) in renal carcinoma cells. We also describe a pipeline designed for the execution and analysis of high content screens. The chemical tools discerned from this screen will allow for the deeper exploration of the mechanism, regulation, and molecular targets of nuclear-localized LC3 in perturbed cellular states. |
format | Online Article Text |
id | pubmed-6018519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-60185192018-06-29 High content screen for identifying small-molecule LC3B-localization modulators in a renal cancer cell line Kolla, Likhitha Heo, David S. Rosenberg, Daniel P. Barlow, Sara A. Maximova, Anna A. Cassio, Emily E. Buchser, William J. Sci Data Data Descriptor Forms of selective autophagy have now been recognized to regulate flux in many intracellular processes. Specific pathways and functions have been identified for mitophagy, ERphagy, and other selective autophagies; yet there is no consensus in whether and how autophagy regulates protein maintenance in and around the nucleus. Such processes are of interest for potential degradation of DNA and nuclear envelope proteins in various disease states. The mechanistic details of such nucleus-related autophagic processes remain elusive due to the lack of chemical or genetic regulators to manipulate and follow the process in vitro. Here, we describe a high content screen from which we identified small chemical compounds that can modulate the localization of the autophagy marker MAP1LC3B (LC3) in renal carcinoma cells. We also describe a pipeline designed for the execution and analysis of high content screens. The chemical tools discerned from this screen will allow for the deeper exploration of the mechanism, regulation, and molecular targets of nuclear-localized LC3 in perturbed cellular states. Nature Publishing Group 2018-06-26 /pmc/articles/PMC6018519/ /pubmed/29944143 http://dx.doi.org/10.1038/sdata.2018.116 Text en Copyright © 2018, The Author(s) http://creativecommons.org/licenses/by/4.0/ Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article. |
spellingShingle | Data Descriptor Kolla, Likhitha Heo, David S. Rosenberg, Daniel P. Barlow, Sara A. Maximova, Anna A. Cassio, Emily E. Buchser, William J. High content screen for identifying small-molecule LC3B-localization modulators in a renal cancer cell line |
title | High content screen for identifying small-molecule LC3B-localization modulators in a renal cancer cell line |
title_full | High content screen for identifying small-molecule LC3B-localization modulators in a renal cancer cell line |
title_fullStr | High content screen for identifying small-molecule LC3B-localization modulators in a renal cancer cell line |
title_full_unstemmed | High content screen for identifying small-molecule LC3B-localization modulators in a renal cancer cell line |
title_short | High content screen for identifying small-molecule LC3B-localization modulators in a renal cancer cell line |
title_sort | high content screen for identifying small-molecule lc3b-localization modulators in a renal cancer cell line |
topic | Data Descriptor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018519/ https://www.ncbi.nlm.nih.gov/pubmed/29944143 http://dx.doi.org/10.1038/sdata.2018.116 |
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