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An antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in Lagria villosa beetles

Microbial symbionts are often a source of chemical novelty and can contribute to host defense against antagonists. However, the ecological relevance of chemical mediators remains unclear for most systems. Lagria beetles live in symbiosis with multiple strains of Burkholderia bacteria that protect th...

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Autores principales: Flórez, Laura V., Scherlach, Kirstin, Miller, Ian J., Rodrigues, Andre, Kwan, Jason C., Hertweck, Christian, Kaltenpoth, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018673/
https://www.ncbi.nlm.nih.gov/pubmed/29946103
http://dx.doi.org/10.1038/s41467-018-04955-6
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author Flórez, Laura V.
Scherlach, Kirstin
Miller, Ian J.
Rodrigues, Andre
Kwan, Jason C.
Hertweck, Christian
Kaltenpoth, Martin
author_facet Flórez, Laura V.
Scherlach, Kirstin
Miller, Ian J.
Rodrigues, Andre
Kwan, Jason C.
Hertweck, Christian
Kaltenpoth, Martin
author_sort Flórez, Laura V.
collection PubMed
description Microbial symbionts are often a source of chemical novelty and can contribute to host defense against antagonists. However, the ecological relevance of chemical mediators remains unclear for most systems. Lagria beetles live in symbiosis with multiple strains of Burkholderia bacteria that protect their offspring against pathogens. Here, we describe the antifungal polyketide lagriamide, and provide evidence supporting that it is produced by an uncultured symbiont, Burkholderia gladioli Lv-StB, which is dominant in field-collected Lagria villosa. Interestingly, lagriamide is structurally similar to bistramides, defensive compounds found in marine tunicates. We identify a gene cluster that is probably involved in lagriamide biosynthesis, provide evidence for horizontal acquisition of these genes, and show that the naturally occurring symbiont strains on the egg are protective in the soil environment. Our findings highlight the potential of microbial symbionts and horizontal gene transfer as influential sources of ecological innovation.
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spelling pubmed-60186732018-06-27 An antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in Lagria villosa beetles Flórez, Laura V. Scherlach, Kirstin Miller, Ian J. Rodrigues, Andre Kwan, Jason C. Hertweck, Christian Kaltenpoth, Martin Nat Commun Article Microbial symbionts are often a source of chemical novelty and can contribute to host defense against antagonists. However, the ecological relevance of chemical mediators remains unclear for most systems. Lagria beetles live in symbiosis with multiple strains of Burkholderia bacteria that protect their offspring against pathogens. Here, we describe the antifungal polyketide lagriamide, and provide evidence supporting that it is produced by an uncultured symbiont, Burkholderia gladioli Lv-StB, which is dominant in field-collected Lagria villosa. Interestingly, lagriamide is structurally similar to bistramides, defensive compounds found in marine tunicates. We identify a gene cluster that is probably involved in lagriamide biosynthesis, provide evidence for horizontal acquisition of these genes, and show that the naturally occurring symbiont strains on the egg are protective in the soil environment. Our findings highlight the potential of microbial symbionts and horizontal gene transfer as influential sources of ecological innovation. Nature Publishing Group UK 2018-06-26 /pmc/articles/PMC6018673/ /pubmed/29946103 http://dx.doi.org/10.1038/s41467-018-04955-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Flórez, Laura V.
Scherlach, Kirstin
Miller, Ian J.
Rodrigues, Andre
Kwan, Jason C.
Hertweck, Christian
Kaltenpoth, Martin
An antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in Lagria villosa beetles
title An antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in Lagria villosa beetles
title_full An antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in Lagria villosa beetles
title_fullStr An antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in Lagria villosa beetles
title_full_unstemmed An antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in Lagria villosa beetles
title_short An antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in Lagria villosa beetles
title_sort antifungal polyketide associated with horizontally acquired genes supports symbiont-mediated defense in lagria villosa beetles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018673/
https://www.ncbi.nlm.nih.gov/pubmed/29946103
http://dx.doi.org/10.1038/s41467-018-04955-6
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