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Viscoelastic properties of Pseudomonas aeruginosa variant biofilms
Pseudomonas aeruginosa evolves during chronic pulmonary infections of cystic fibrosis (CF) patients, forming pathoadapted variants that are persistent. Mucoid and rugose small-colony variants (RSCVs) are typically isolated from sputum of CF patients. These variants overproduce exopolysaccharides in...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018706/ https://www.ncbi.nlm.nih.gov/pubmed/29946126 http://dx.doi.org/10.1038/s41598-018-28009-5 |
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author | Gloag, Erin S. German, Guy K. Stoodley, Paul Wozniak, Daniel J. |
author_facet | Gloag, Erin S. German, Guy K. Stoodley, Paul Wozniak, Daniel J. |
author_sort | Gloag, Erin S. |
collection | PubMed |
description | Pseudomonas aeruginosa evolves during chronic pulmonary infections of cystic fibrosis (CF) patients, forming pathoadapted variants that are persistent. Mucoid and rugose small-colony variants (RSCVs) are typically isolated from sputum of CF patients. These variants overproduce exopolysaccharides in the biofilm extracellular polymeric substance (EPS). Currently, changes to the biophysical properties of RSCV and mucoid biofilms due to variations in EPS are not well understood. This knowledge may reveal how lung infections resist host clearance mechanisms. Here, we used mechanical indentation and shear rheometry to analyse the viscoelasticity of RSCV and mucoid colony-biofilms compared to their isogenic parent at 2-, 4-, and 6-d. While the viscoelasticity of parental colony-biofilms underwent fluctuating temporal changes, in contrast, RSCV and mucoid colony-biofilms showed a gradual progression to more elastic-solid behaviour. Theoretical indices of mucociliary and cough clearance predict that mature 6-d parental and RSCV biofilms may show reduced cough clearance from the lung, while early mucoid biofilms may show reduced clearance by both mechanisms. We propose that viscoelasticity be considered a virulence property of biofilms. |
format | Online Article Text |
id | pubmed-6018706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-60187062018-07-06 Viscoelastic properties of Pseudomonas aeruginosa variant biofilms Gloag, Erin S. German, Guy K. Stoodley, Paul Wozniak, Daniel J. Sci Rep Article Pseudomonas aeruginosa evolves during chronic pulmonary infections of cystic fibrosis (CF) patients, forming pathoadapted variants that are persistent. Mucoid and rugose small-colony variants (RSCVs) are typically isolated from sputum of CF patients. These variants overproduce exopolysaccharides in the biofilm extracellular polymeric substance (EPS). Currently, changes to the biophysical properties of RSCV and mucoid biofilms due to variations in EPS are not well understood. This knowledge may reveal how lung infections resist host clearance mechanisms. Here, we used mechanical indentation and shear rheometry to analyse the viscoelasticity of RSCV and mucoid colony-biofilms compared to their isogenic parent at 2-, 4-, and 6-d. While the viscoelasticity of parental colony-biofilms underwent fluctuating temporal changes, in contrast, RSCV and mucoid colony-biofilms showed a gradual progression to more elastic-solid behaviour. Theoretical indices of mucociliary and cough clearance predict that mature 6-d parental and RSCV biofilms may show reduced cough clearance from the lung, while early mucoid biofilms may show reduced clearance by both mechanisms. We propose that viscoelasticity be considered a virulence property of biofilms. Nature Publishing Group UK 2018-06-26 /pmc/articles/PMC6018706/ /pubmed/29946126 http://dx.doi.org/10.1038/s41598-018-28009-5 Text en © The Author(s) 2018 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Gloag, Erin S. German, Guy K. Stoodley, Paul Wozniak, Daniel J. Viscoelastic properties of Pseudomonas aeruginosa variant biofilms |
title | Viscoelastic properties of Pseudomonas aeruginosa variant biofilms |
title_full | Viscoelastic properties of Pseudomonas aeruginosa variant biofilms |
title_fullStr | Viscoelastic properties of Pseudomonas aeruginosa variant biofilms |
title_full_unstemmed | Viscoelastic properties of Pseudomonas aeruginosa variant biofilms |
title_short | Viscoelastic properties of Pseudomonas aeruginosa variant biofilms |
title_sort | viscoelastic properties of pseudomonas aeruginosa variant biofilms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018706/ https://www.ncbi.nlm.nih.gov/pubmed/29946126 http://dx.doi.org/10.1038/s41598-018-28009-5 |
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