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Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid

While CRISPR-Cas9-mediated genome editing has transformed yeast research, current plasmids and cassettes for Cas9 and guide-RNA expression are species specific. CRISPR tools that function in multiple yeast species could contribute to the intensifying research on non-conventional yeasts. A plasmid ca...

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Detalles Bibliográficos
Autores principales:	Juergens, Hannes, Varela, Javier A, Gorter de Vries, Arthur R, Perli, Thomas, Gast, Veronica J M, Gyurchev, Nikola Y, Rajkumar, Arun S, Mans, Robert, Pronk, Jack T, Morrissey, John P, Daran, Jean-Marc G
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018904/ https://www.ncbi.nlm.nih.gov/pubmed/29438517 http://dx.doi.org/10.1093/femsyr/foy012

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