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Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato

The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes. Starch and amylose...

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Autores principales: Andersson, Mariette, Turesson, Helle, Arrivault, Stéphanie, Zhang, Youjun, Fält, Ann-Sofie, Fernie, Alisdair R, Hofvander, Per
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018912/
https://www.ncbi.nlm.nih.gov/pubmed/29538769
http://dx.doi.org/10.1093/jxb/ery051
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author Andersson, Mariette
Turesson, Helle
Arrivault, Stéphanie
Zhang, Youjun
Fält, Ann-Sofie
Fernie, Alisdair R
Hofvander, Per
author_facet Andersson, Mariette
Turesson, Helle
Arrivault, Stéphanie
Zhang, Youjun
Fält, Ann-Sofie
Fernie, Alisdair R
Hofvander, Per
author_sort Andersson, Mariette
collection PubMed
description The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes. Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects. Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield. In contrast, when both genes were inhibited simultaneously, some lines developed only a few, small and distorted tubers. Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines. Increased amounts of intermediates of vitamin C biosynthesis were also observed. This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue. Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development.
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spelling pubmed-60189122018-07-20 Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato Andersson, Mariette Turesson, Helle Arrivault, Stéphanie Zhang, Youjun Fält, Ann-Sofie Fernie, Alisdair R Hofvander, Per J Exp Bot Research Paper The importance of a plastidial soluble inorganic pyrophosphatase (psPPase) and an ATP/ADP translocator (NTT) for starch composition and tuber formation in potato (Solanum tuberosum) was evaluated by individual and simultaneous down-regulation of the corresponding endogenous genes. Starch and amylose content of the transgenic lines were considerably lower, and granule size substantially smaller, with down-regulation of StpsPPase generating the most pronounced effects. Single-gene down-regulation of either StpsPPase or StNTT resulted in increased tuber numbers per plant and higher fresh weight yield. In contrast, when both genes were inhibited simultaneously, some lines developed only a few, small and distorted tubers. Analysis of metabolites revealed altered amounts of sugar intermediates, and a substantial increase in ADP-glucose content of the StpsPPase lines. Increased amounts of intermediates of vitamin C biosynthesis were also observed. This study suggests that hydrolysis of pyrophosphate (PPi) by action of a psPPase is vital for functional starch accumulation in potato tubers and that no additional mechanism for consuming, hydrolysing, or exporting PPi exists in the studied tissue. Additionally, it demonstrates that functional PPi hydrolysis in combination with efficient ATP import is essential for tuber formation and development. Oxford University Press 2018-04-03 2018-03-10 /pmc/articles/PMC6018912/ /pubmed/29538769 http://dx.doi.org/10.1093/jxb/ery051 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Andersson, Mariette
Turesson, Helle
Arrivault, Stéphanie
Zhang, Youjun
Fält, Ann-Sofie
Fernie, Alisdair R
Hofvander, Per
Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato
title Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato
title_full Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato
title_fullStr Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato
title_full_unstemmed Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato
title_short Inhibition of plastid PPase and NTT leads to major changes in starch and tuber formation in potato
title_sort inhibition of plastid ppase and ntt leads to major changes in starch and tuber formation in potato
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018912/
https://www.ncbi.nlm.nih.gov/pubmed/29538769
http://dx.doi.org/10.1093/jxb/ery051
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