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Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy
Galactinol synthase (GolS) is a key enzyme in the biosynthetic pathway of raffinose family oligosaccharides (RFOs), which play roles in carbon storage, signal transduction, and osmoprotection. The present work assessed the evolutionary history of GolS genes across the Rosaceae using several bioinfor...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018919/ https://www.ncbi.nlm.nih.gov/pubmed/29373762 http://dx.doi.org/10.1093/jxb/erx451 |
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author | Falavigna, Vítor da Silveira Porto, Diogo Denardi Miotto, Yohanna Evelyn dos Santos, Henrique Pessoa de Oliveira, Paulo Ricardo Dias Margis-Pinheiro, Márcia Pasquali, Giancarlo Revers, Luís Fernando |
author_facet | Falavigna, Vítor da Silveira Porto, Diogo Denardi Miotto, Yohanna Evelyn dos Santos, Henrique Pessoa de Oliveira, Paulo Ricardo Dias Margis-Pinheiro, Márcia Pasquali, Giancarlo Revers, Luís Fernando |
author_sort | Falavigna, Vítor da Silveira |
collection | PubMed |
description | Galactinol synthase (GolS) is a key enzyme in the biosynthetic pathway of raffinose family oligosaccharides (RFOs), which play roles in carbon storage, signal transduction, and osmoprotection. The present work assessed the evolutionary history of GolS genes across the Rosaceae using several bioinformatic tools. Apple (Malus × domestica) GolS genes were transcriptionally characterized during bud dormancy, in parallel with galactinol and raffinose measurements. Additionally, MdGolS2, a candidate to regulate seasonal galactinol and RFO content during apple bud dormancy, was functionally characterized in Arabidopsis. Evolutionary analyses revealed that whole genome duplications have driven GolS gene evolution and diversification in Rosaceae speciation. The strong purifying selection identified in duplicated GolS genes suggests that differential gene expression might define gene function better than protein structure. Interestingly, MdGolS2 was differentially expressed during bud dormancy, concomitantly with the highest galactinol and raffinose levels. One of the intrinsic adaptive features of bud dormancy is limited availability of free water; therefore, we generated transgenic Arabidopsis plants expressing MdGolS2. They showed higher galactinol and raffinose contents and increased tolerance to water deficit. Our results suggest that MdGolS2 is the major GolS responsible for RFO accumulation during apple dormancy, and these carbohydrates help to protect dormant buds against limited water supply. |
format | Online Article Text |
id | pubmed-6018919 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60189192018-07-10 Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy Falavigna, Vítor da Silveira Porto, Diogo Denardi Miotto, Yohanna Evelyn dos Santos, Henrique Pessoa de Oliveira, Paulo Ricardo Dias Margis-Pinheiro, Márcia Pasquali, Giancarlo Revers, Luís Fernando J Exp Bot Research Papers Galactinol synthase (GolS) is a key enzyme in the biosynthetic pathway of raffinose family oligosaccharides (RFOs), which play roles in carbon storage, signal transduction, and osmoprotection. The present work assessed the evolutionary history of GolS genes across the Rosaceae using several bioinformatic tools. Apple (Malus × domestica) GolS genes were transcriptionally characterized during bud dormancy, in parallel with galactinol and raffinose measurements. Additionally, MdGolS2, a candidate to regulate seasonal galactinol and RFO content during apple bud dormancy, was functionally characterized in Arabidopsis. Evolutionary analyses revealed that whole genome duplications have driven GolS gene evolution and diversification in Rosaceae speciation. The strong purifying selection identified in duplicated GolS genes suggests that differential gene expression might define gene function better than protein structure. Interestingly, MdGolS2 was differentially expressed during bud dormancy, concomitantly with the highest galactinol and raffinose levels. One of the intrinsic adaptive features of bud dormancy is limited availability of free water; therefore, we generated transgenic Arabidopsis plants expressing MdGolS2. They showed higher galactinol and raffinose contents and increased tolerance to water deficit. Our results suggest that MdGolS2 is the major GolS responsible for RFO accumulation during apple dormancy, and these carbohydrates help to protect dormant buds against limited water supply. Oxford University Press 2018-02-20 2018-01-24 /pmc/articles/PMC6018919/ /pubmed/29373762 http://dx.doi.org/10.1093/jxb/erx451 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Falavigna, Vítor da Silveira Porto, Diogo Denardi Miotto, Yohanna Evelyn dos Santos, Henrique Pessoa de Oliveira, Paulo Ricardo Dias Margis-Pinheiro, Márcia Pasquali, Giancarlo Revers, Luís Fernando Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy |
title | Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy |
title_full | Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy |
title_fullStr | Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy |
title_full_unstemmed | Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy |
title_short | Evolutionary diversification of galactinol synthases in Rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy |
title_sort | evolutionary diversification of galactinol synthases in rosaceae: adaptive roles of galactinol and raffinose during apple bud dormancy |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018919/ https://www.ncbi.nlm.nih.gov/pubmed/29373762 http://dx.doi.org/10.1093/jxb/erx451 |
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