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Attenuated accumulation of jasmonates modifies stomatal responses to water deficit

To determine whether drought-induced root jasmonate [jasmonic acid (JA) and jasmonic acid-isoleucine (JA-Ile)] accumulation affected shoot responses to drying soil, near-isogenic wild-type (WT) tomato (Solanum lycopersicum cv. Castlemart) and the def-1 mutant (which fails to accumulate jasmonates du...

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Autores principales: De Ollas, Carlos, Arbona, Vicent, Gómez-Cadenas, Aurelio, Dodd, Ian C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018964/
https://www.ncbi.nlm.nih.gov/pubmed/29432619
http://dx.doi.org/10.1093/jxb/ery045
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author De Ollas, Carlos
Arbona, Vicent
Gómez-Cadenas, Aurelio
Dodd, Ian C
author_facet De Ollas, Carlos
Arbona, Vicent
Gómez-Cadenas, Aurelio
Dodd, Ian C
author_sort De Ollas, Carlos
collection PubMed
description To determine whether drought-induced root jasmonate [jasmonic acid (JA) and jasmonic acid-isoleucine (JA-Ile)] accumulation affected shoot responses to drying soil, near-isogenic wild-type (WT) tomato (Solanum lycopersicum cv. Castlemart) and the def-1 mutant (which fails to accumulate jasmonates during water deficit) were self- and reciprocally grafted. Rootstock hydraulic conductance was entirely rootstock dependent and significantly lower in def-1, yet def-1 scions maintained a higher leaf water potential as the soil dried due to their lower stomatal conductance (g(s)). Stomatal sensitivity to drying soil (the slope of g(s)versus soil water content) was low in def-1 self-grafts but was normalized by grafting onto WT rootstocks. Although soil drying increased 12-oxo-phytodienoic acid (OPDA; a JA precursor and putative antitranspirant) concentrations in def-1 scions, foliar JA accumulation was negligible and foliar ABA accumulation reduced compared with WT scions. A WT rootstock increased drought-induced ABA and JA accumulation in def-1 scions, but decreased OPDA accumulation. Xylem-borne jasmonates were biologically active, since supplying exogenous JA via the transpiration stream to detached leaves decreased transpiration of WT seedlings but had the opposite effect in def-1. Thus foliar accumulation of both ABA and JA at WT levels is required for both maximum (well-watered) g(s) and stomatal sensitivity to drying soil.
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spelling pubmed-60189642018-07-20 Attenuated accumulation of jasmonates modifies stomatal responses to water deficit De Ollas, Carlos Arbona, Vicent Gómez-Cadenas, Aurelio Dodd, Ian C J Exp Bot Research Paper To determine whether drought-induced root jasmonate [jasmonic acid (JA) and jasmonic acid-isoleucine (JA-Ile)] accumulation affected shoot responses to drying soil, near-isogenic wild-type (WT) tomato (Solanum lycopersicum cv. Castlemart) and the def-1 mutant (which fails to accumulate jasmonates during water deficit) were self- and reciprocally grafted. Rootstock hydraulic conductance was entirely rootstock dependent and significantly lower in def-1, yet def-1 scions maintained a higher leaf water potential as the soil dried due to their lower stomatal conductance (g(s)). Stomatal sensitivity to drying soil (the slope of g(s)versus soil water content) was low in def-1 self-grafts but was normalized by grafting onto WT rootstocks. Although soil drying increased 12-oxo-phytodienoic acid (OPDA; a JA precursor and putative antitranspirant) concentrations in def-1 scions, foliar JA accumulation was negligible and foliar ABA accumulation reduced compared with WT scions. A WT rootstock increased drought-induced ABA and JA accumulation in def-1 scions, but decreased OPDA accumulation. Xylem-borne jasmonates were biologically active, since supplying exogenous JA via the transpiration stream to detached leaves decreased transpiration of WT seedlings but had the opposite effect in def-1. Thus foliar accumulation of both ABA and JA at WT levels is required for both maximum (well-watered) g(s) and stomatal sensitivity to drying soil. Oxford University Press 2018-04-03 2018-02-08 /pmc/articles/PMC6018964/ /pubmed/29432619 http://dx.doi.org/10.1093/jxb/ery045 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
De Ollas, Carlos
Arbona, Vicent
Gómez-Cadenas, Aurelio
Dodd, Ian C
Attenuated accumulation of jasmonates modifies stomatal responses to water deficit
title Attenuated accumulation of jasmonates modifies stomatal responses to water deficit
title_full Attenuated accumulation of jasmonates modifies stomatal responses to water deficit
title_fullStr Attenuated accumulation of jasmonates modifies stomatal responses to water deficit
title_full_unstemmed Attenuated accumulation of jasmonates modifies stomatal responses to water deficit
title_short Attenuated accumulation of jasmonates modifies stomatal responses to water deficit
title_sort attenuated accumulation of jasmonates modifies stomatal responses to water deficit
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6018964/
https://www.ncbi.nlm.nih.gov/pubmed/29432619
http://dx.doi.org/10.1093/jxb/ery045
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