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The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells

BACKGROUND: One of the molecular mechanisms involved in upper airway-related diseases is epithelial-to-mesenchymal transition (EMT). Olea europaea (OE) has anti-inflammatory properties and thus, great potential to prevent EMT. This study aimed to investigate the effect of OE on EMT in primary nasal...

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Autores principales: Razali, Rabiatul Adawiyah, Nik Ahmad Eid, Nik Ahmad Hafiz, Jayaraman, Turkambigai, Amir Hassan, Muhammad Asyrafi, Azlan, Nabilah Qistina, Ismail, Nur Farhana, Sainik, Nur Qisya Afifah Veronica, Yazid, Muhammad Dain, Lokanathan, Yogeswaran, Saim, Aminuddin Bin, Hj Idrus, Ruszymah Bt
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019306/
https://www.ncbi.nlm.nih.gov/pubmed/29940929
http://dx.doi.org/10.1186/s12906-018-2250-5
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author Razali, Rabiatul Adawiyah
Nik Ahmad Eid, Nik Ahmad Hafiz
Jayaraman, Turkambigai
Amir Hassan, Muhammad Asyrafi
Azlan, Nabilah Qistina
Ismail, Nur Farhana
Sainik, Nur Qisya Afifah Veronica
Yazid, Muhammad Dain
Lokanathan, Yogeswaran
Saim, Aminuddin Bin
Hj Idrus, Ruszymah Bt
author_facet Razali, Rabiatul Adawiyah
Nik Ahmad Eid, Nik Ahmad Hafiz
Jayaraman, Turkambigai
Amir Hassan, Muhammad Asyrafi
Azlan, Nabilah Qistina
Ismail, Nur Farhana
Sainik, Nur Qisya Afifah Veronica
Yazid, Muhammad Dain
Lokanathan, Yogeswaran
Saim, Aminuddin Bin
Hj Idrus, Ruszymah Bt
author_sort Razali, Rabiatul Adawiyah
collection PubMed
description BACKGROUND: One of the molecular mechanisms involved in upper airway-related diseases is epithelial-to-mesenchymal transition (EMT). Olea europaea (OE) has anti-inflammatory properties and thus, great potential to prevent EMT. This study aimed to investigate the effect of OE on EMT in primary nasal human respiratory epithelial cells (RECs). METHODS: Respiratory epithelial cells were isolated and divided into four groups: control (untreated), treated with 0.05% OE (OE group), EMT induced with 5 ng/ml of transforming growth factor beta-1 (TGFβ1 group) and treated with 5 ng/ml TGFβ1 + 0.05% OE (TGFβ1 + OE group). The effects of OE treatment on growth kinetics, morphology and protein expression in RECs were evaluated. Immunocytochemistry analysis was performed to quantitate the total percentage of E-cadherin and vimentin expression from day 1 to day 3. RESULTS: There were no significant differences between untreated RECs and OE-treated RECs in terms of their morphology, growth kinetics and protein expression. Induction with TGFβ1 caused RECs to have an elongated spindle shape, a slower proliferation rate, a higher expression of vimentin and a lower expression of E-cadherin compared with the control. Cells in the TGFβ1 + OE group had similar epithelial shape to untreated group however it had no significant differences in their proliferation rate when compared to TGFβ1-induced RECs. Cells treated with TGFβ1 + OE showed significantly reduced expression of vimentin and increased expression of E-cadherin compared with the TGFβ1 group (P < 0.05). CONCLUSION: The ability of OE to inhibit EMT in RECs was shown by TGFb1-induced EMT REC morphology, growth kinetics and protein expression markers (E-cadherin and vimentin) upon treatment with OE and TGFβ1. Therefore, this study could provide insight into the therapeutic potential of OE to inhibit pathological tissue remodelling and persistent inflammation.
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spelling pubmed-60193062018-07-06 The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells Razali, Rabiatul Adawiyah Nik Ahmad Eid, Nik Ahmad Hafiz Jayaraman, Turkambigai Amir Hassan, Muhammad Asyrafi Azlan, Nabilah Qistina Ismail, Nur Farhana Sainik, Nur Qisya Afifah Veronica Yazid, Muhammad Dain Lokanathan, Yogeswaran Saim, Aminuddin Bin Hj Idrus, Ruszymah Bt BMC Complement Altern Med Research Article BACKGROUND: One of the molecular mechanisms involved in upper airway-related diseases is epithelial-to-mesenchymal transition (EMT). Olea europaea (OE) has anti-inflammatory properties and thus, great potential to prevent EMT. This study aimed to investigate the effect of OE on EMT in primary nasal human respiratory epithelial cells (RECs). METHODS: Respiratory epithelial cells were isolated and divided into four groups: control (untreated), treated with 0.05% OE (OE group), EMT induced with 5 ng/ml of transforming growth factor beta-1 (TGFβ1 group) and treated with 5 ng/ml TGFβ1 + 0.05% OE (TGFβ1 + OE group). The effects of OE treatment on growth kinetics, morphology and protein expression in RECs were evaluated. Immunocytochemistry analysis was performed to quantitate the total percentage of E-cadherin and vimentin expression from day 1 to day 3. RESULTS: There were no significant differences between untreated RECs and OE-treated RECs in terms of their morphology, growth kinetics and protein expression. Induction with TGFβ1 caused RECs to have an elongated spindle shape, a slower proliferation rate, a higher expression of vimentin and a lower expression of E-cadherin compared with the control. Cells in the TGFβ1 + OE group had similar epithelial shape to untreated group however it had no significant differences in their proliferation rate when compared to TGFβ1-induced RECs. Cells treated with TGFβ1 + OE showed significantly reduced expression of vimentin and increased expression of E-cadherin compared with the TGFβ1 group (P < 0.05). CONCLUSION: The ability of OE to inhibit EMT in RECs was shown by TGFb1-induced EMT REC morphology, growth kinetics and protein expression markers (E-cadherin and vimentin) upon treatment with OE and TGFβ1. Therefore, this study could provide insight into the therapeutic potential of OE to inhibit pathological tissue remodelling and persistent inflammation. BioMed Central 2018-06-26 /pmc/articles/PMC6019306/ /pubmed/29940929 http://dx.doi.org/10.1186/s12906-018-2250-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Razali, Rabiatul Adawiyah
Nik Ahmad Eid, Nik Ahmad Hafiz
Jayaraman, Turkambigai
Amir Hassan, Muhammad Asyrafi
Azlan, Nabilah Qistina
Ismail, Nur Farhana
Sainik, Nur Qisya Afifah Veronica
Yazid, Muhammad Dain
Lokanathan, Yogeswaran
Saim, Aminuddin Bin
Hj Idrus, Ruszymah Bt
The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells
title The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells
title_full The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells
title_fullStr The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells
title_full_unstemmed The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells
title_short The potential of Olea europaea extracts to prevent TGFβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells
title_sort potential of olea europaea extracts to prevent tgfβ1-induced epithelial to mesenchymal transition in human nasal respiratory epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019306/
https://www.ncbi.nlm.nih.gov/pubmed/29940929
http://dx.doi.org/10.1186/s12906-018-2250-5
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