高分辨率熔解曲线法在BIM基因2, 903 bp片段插入/缺失检测中的应用

BACKGROUND AND OBJECTIVE: The aim of this study is to establish a HRM (high resolution melting curve) method for detection of deletion in human BIM gene and to detect this site deletion with the above method in 30 lung cancer samples and 30 normal samples. METHODS: The primers for detection of BIM deletion were designed and synthesized. The HRM method for geng deletion was established. And select the part of samples to detect BIM delection by normal PCR and sequencing assay. The Tm value of wild type PCR products was higher than that of the deletion PCR products. The difference of the corresponding Tm value is 2.5 ℃. RESULTS: By detection with HRM methods, 1 samples were confirmed to be mutant, 7 samples were confirmed to be heterozygous and the other 22 samples were all wild type in the lung cancer samples. 2 samples were confirmed to be heterozygous and the other 28 samples were all wild type in the normal samples. CONCLUSION: The HRM method for detection of BIM deletion established in this study is a sensitive, accurate, simple and high throughput method.