Cargando…

Antennal Protein Profile in Honeybees: Caste and Task Matter More Than Age

Reproductive and task partitioning in large colonies of social insects suggest that colony members belonging to different castes or performing different tasks during their life (polyethism) may produce specific semiochemicals and be differently sensitive to the variety of pheromones involved in intr...

Descripción completa

Detalles Bibliográficos
Autores principales: Iovinella, Immacolata, Cappa, Federico, Cini, Alessandro, Petrocelli, Iacopo, Cervo, Rita, Turillazzi, Stefano, Dani, Francesca R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019485/
https://www.ncbi.nlm.nih.gov/pubmed/29973886
http://dx.doi.org/10.3389/fphys.2018.00748
_version_ 1783335133318742016
author Iovinella, Immacolata
Cappa, Federico
Cini, Alessandro
Petrocelli, Iacopo
Cervo, Rita
Turillazzi, Stefano
Dani, Francesca R.
author_facet Iovinella, Immacolata
Cappa, Federico
Cini, Alessandro
Petrocelli, Iacopo
Cervo, Rita
Turillazzi, Stefano
Dani, Francesca R.
author_sort Iovinella, Immacolata
collection PubMed
description Reproductive and task partitioning in large colonies of social insects suggest that colony members belonging to different castes or performing different tasks during their life (polyethism) may produce specific semiochemicals and be differently sensitive to the variety of pheromones involved in intraspecific chemical communication. The main peripheral olfactory organs are the antennal chemosensilla, where the early olfactory processes take place. At this stage, members of two different families of soluble chemosensory proteins [odorant-binding proteins (OBPs) and chemosensory proteins (CSPs)] show a remarkable affinity for different odorants and act as carriers while a further family, the Niemann-Pick type C2 proteins (NPC2) may have a similar function, although this has not been fully demonstrated. Sensillar lymph also contains Odorant degrading enzymes (ODEs) which are involved in inactivation through degradation of the chemical signals, once the message is conveyed. Despite their importance in chemical communication, little is known about how proteins involved in peripheral olfaction and, more generally antennal proteins, differ in honeybees of different caste, task and age. Here, we investigate for the first time, using a shotgun proteomic approach, the antennal profile of honeybees of different castes (queens and workers) and workers performing different tasks (nurses, guards, and foragers) by controlling for the potential confounding effect of age. Regarding olfactory proteins, major differences were observed between queens and workers, some of which were found to be more abundant in queens (OBP3, OBP18, and NPC2-1) and others to be more abundant in workers (OBP15, OBP21, CSP1, and CSP3); while between workers performing different tasks, OBP14 was more abundant in nurses with respect to guards and foragers. Apart from proteins involved in olfaction, we have found that the antennal proteomes are mainly characterized by castes and tasks, while age has no effect on antennal protein profile. Among the main differences, the strong decrease in vitellogenins found in guards and foragers is not associated with age.
format Online
Article
Text
id pubmed-6019485
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-60194852018-07-04 Antennal Protein Profile in Honeybees: Caste and Task Matter More Than Age Iovinella, Immacolata Cappa, Federico Cini, Alessandro Petrocelli, Iacopo Cervo, Rita Turillazzi, Stefano Dani, Francesca R. Front Physiol Physiology Reproductive and task partitioning in large colonies of social insects suggest that colony members belonging to different castes or performing different tasks during their life (polyethism) may produce specific semiochemicals and be differently sensitive to the variety of pheromones involved in intraspecific chemical communication. The main peripheral olfactory organs are the antennal chemosensilla, where the early olfactory processes take place. At this stage, members of two different families of soluble chemosensory proteins [odorant-binding proteins (OBPs) and chemosensory proteins (CSPs)] show a remarkable affinity for different odorants and act as carriers while a further family, the Niemann-Pick type C2 proteins (NPC2) may have a similar function, although this has not been fully demonstrated. Sensillar lymph also contains Odorant degrading enzymes (ODEs) which are involved in inactivation through degradation of the chemical signals, once the message is conveyed. Despite their importance in chemical communication, little is known about how proteins involved in peripheral olfaction and, more generally antennal proteins, differ in honeybees of different caste, task and age. Here, we investigate for the first time, using a shotgun proteomic approach, the antennal profile of honeybees of different castes (queens and workers) and workers performing different tasks (nurses, guards, and foragers) by controlling for the potential confounding effect of age. Regarding olfactory proteins, major differences were observed between queens and workers, some of which were found to be more abundant in queens (OBP3, OBP18, and NPC2-1) and others to be more abundant in workers (OBP15, OBP21, CSP1, and CSP3); while between workers performing different tasks, OBP14 was more abundant in nurses with respect to guards and foragers. Apart from proteins involved in olfaction, we have found that the antennal proteomes are mainly characterized by castes and tasks, while age has no effect on antennal protein profile. Among the main differences, the strong decrease in vitellogenins found in guards and foragers is not associated with age. Frontiers Media S.A. 2018-06-20 /pmc/articles/PMC6019485/ /pubmed/29973886 http://dx.doi.org/10.3389/fphys.2018.00748 Text en Copyright © 2018 Iovinella, Cappa, Cini, Petrocelli, Cervo, Turillazzi and Dani. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Iovinella, Immacolata
Cappa, Federico
Cini, Alessandro
Petrocelli, Iacopo
Cervo, Rita
Turillazzi, Stefano
Dani, Francesca R.
Antennal Protein Profile in Honeybees: Caste and Task Matter More Than Age
title Antennal Protein Profile in Honeybees: Caste and Task Matter More Than Age
title_full Antennal Protein Profile in Honeybees: Caste and Task Matter More Than Age
title_fullStr Antennal Protein Profile in Honeybees: Caste and Task Matter More Than Age
title_full_unstemmed Antennal Protein Profile in Honeybees: Caste and Task Matter More Than Age
title_short Antennal Protein Profile in Honeybees: Caste and Task Matter More Than Age
title_sort antennal protein profile in honeybees: caste and task matter more than age
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019485/
https://www.ncbi.nlm.nih.gov/pubmed/29973886
http://dx.doi.org/10.3389/fphys.2018.00748
work_keys_str_mv AT iovinellaimmacolata antennalproteinprofileinhoneybeescasteandtaskmattermorethanage
AT cappafederico antennalproteinprofileinhoneybeescasteandtaskmattermorethanage
AT cinialessandro antennalproteinprofileinhoneybeescasteandtaskmattermorethanage
AT petrocelliiacopo antennalproteinprofileinhoneybeescasteandtaskmattermorethanage
AT cervorita antennalproteinprofileinhoneybeescasteandtaskmattermorethanage
AT turillazzistefano antennalproteinprofileinhoneybeescasteandtaskmattermorethanage
AT danifrancescar antennalproteinprofileinhoneybeescasteandtaskmattermorethanage