Molecular Evaluation of a Case of Fasciola hepatica in Wild Boar in Southwestern Iran: A Case Report

Wild boars may be infected with several zoonotic parasitic infections including Fasciola spp. We reported a case of Fasciola infection in a wild boar in Bushehr Province in southwestern Iran. The sample was isolated from the liver of a hunted wild boar. A few of adult worms were fixed and stained. D...

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Autores principales: SARKARI, Bahador, MANSOURI, Majid, NOORPISHEH GHADIMI, Shamsi, ABDOLAHI KHABISI, Samaneh, DOSHMANZIARI, Abdolla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2018
Materias:
Cases Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019598/
https://www.ncbi.nlm.nih.gov/pubmed/29963098
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author SARKARI, Bahador
MANSOURI, Majid
NOORPISHEH GHADIMI, Shamsi
ABDOLAHI KHABISI, Samaneh
DOSHMANZIARI, Abdolla
author_facet SARKARI, Bahador
MANSOURI, Majid
NOORPISHEH GHADIMI, Shamsi
ABDOLAHI KHABISI, Samaneh
DOSHMANZIARI, Abdolla
author_sort SARKARI, Bahador
collection PubMed
description Wild boars may be infected with several zoonotic parasitic infections including Fasciola spp. We reported a case of Fasciola infection in a wild boar in Bushehr Province in southwestern Iran. The sample was isolated from the liver of a hunted wild boar. A few of adult worms were fixed and stained. DNA was extracted from apical and lateral parts of the worms and PCR amplified, targeting NADH dehydrogenase subunit 1 (nad1) and cytochrome C oxidase subunit 1 (cox1) mitochondrion genes. Although the worm was quite long and looked much similar to F. gigantica, sequencing and analysis of PCR products of nad1 and cox1 genes revealed that the isolate has the most similarity with F. hepatica. This is the first report of molecular evaluation of Fasciola spp. from wild boar in Iran.
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spelling pubmed-60195982018-06-29 Molecular Evaluation of a Case of Fasciola hepatica in Wild Boar in Southwestern Iran: A Case Report SARKARI, Bahador MANSOURI, Majid NOORPISHEH GHADIMI, Shamsi ABDOLAHI KHABISI, Samaneh DOSHMANZIARI, Abdolla Iran J Parasitol Cases Report Wild boars may be infected with several zoonotic parasitic infections including Fasciola spp. We reported a case of Fasciola infection in a wild boar in Bushehr Province in southwestern Iran. The sample was isolated from the liver of a hunted wild boar. A few of adult worms were fixed and stained. DNA was extracted from apical and lateral parts of the worms and PCR amplified, targeting NADH dehydrogenase subunit 1 (nad1) and cytochrome C oxidase subunit 1 (cox1) mitochondrion genes. Although the worm was quite long and looked much similar to F. gigantica, sequencing and analysis of PCR products of nad1 and cox1 genes revealed that the isolate has the most similarity with F. hepatica. This is the first report of molecular evaluation of Fasciola spp. from wild boar in Iran. Tehran University of Medical Sciences 2018 /pmc/articles/PMC6019598/ /pubmed/29963098 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cases Report
SARKARI, Bahador
MANSOURI, Majid
NOORPISHEH GHADIMI, Shamsi
ABDOLAHI KHABISI, Samaneh
DOSHMANZIARI, Abdolla
Molecular Evaluation of a Case of Fasciola hepatica in Wild Boar in Southwestern Iran: A Case Report
title Molecular Evaluation of a Case of Fasciola hepatica in Wild Boar in Southwestern Iran: A Case Report
title_full Molecular Evaluation of a Case of Fasciola hepatica in Wild Boar in Southwestern Iran: A Case Report
title_fullStr Molecular Evaluation of a Case of Fasciola hepatica in Wild Boar in Southwestern Iran: A Case Report
title_full_unstemmed Molecular Evaluation of a Case of Fasciola hepatica in Wild Boar in Southwestern Iran: A Case Report
title_short Molecular Evaluation of a Case of Fasciola hepatica in Wild Boar in Southwestern Iran: A Case Report
title_sort molecular evaluation of a case of fasciola hepatica in wild boar in southwestern iran: a case report
topic Cases Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019598/
https://www.ncbi.nlm.nih.gov/pubmed/29963098
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