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Nonaplex PCR using Cliffhanger primers to identify diarrhoeagenic Escherichia coli from crude lysates of human faecal samples

Sensitive, probe-based detection of multiple DNA targets is limited by the competitive reannealing of the antiparallel duplex DNA helix with the complementary DNA strand. To address this, we developed Cliffhanger primers, which create single-stranded DNA overhangs on PCR amplicons while simultaneous...

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Detalles Bibliográficos
Autores principales:	Schneider, Uffe Vest, Mikkelsen, Nikolaj Dam, Scheutz, Flemming, Friis-Møller, Alice, Lisby, Gorm
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019694/ https://www.ncbi.nlm.nih.gov/pubmed/29944710 http://dx.doi.org/10.1371/journal.pone.0199766

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