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Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection

BACKGROUND: Control of Mycobacterium tuberculosis (Mtb) infection requires CD4(+) T-cell responses and major histocompatibility complex class II (MHC II) presentation of Mtb antigens (Ags). Dendritic cells (DCs) are the most potent of the Ag-presenting cells and are central to the initiation of T-ce...

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Autores principales: Xu, Zhengzhong, Xia, Aihong, Li, Xin, Zhu, Zhaocheng, Shen, Yechi, Jin, Shanshan, Lan, Tian, Xie, Yuqing, Wu, Han, Meng, Chuang, Sun, Lin, Yin, Yuelan, Chen, Xiang, Jiao, Xinan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019797/
https://www.ncbi.nlm.nih.gov/pubmed/29940854
http://dx.doi.org/10.1186/s12865-018-0258-8
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author Xu, Zhengzhong
Xia, Aihong
Li, Xin
Zhu, Zhaocheng
Shen, Yechi
Jin, Shanshan
Lan, Tian
Xie, Yuqing
Wu, Han
Meng, Chuang
Sun, Lin
Yin, Yuelan
Chen, Xiang
Jiao, Xinan
author_facet Xu, Zhengzhong
Xia, Aihong
Li, Xin
Zhu, Zhaocheng
Shen, Yechi
Jin, Shanshan
Lan, Tian
Xie, Yuqing
Wu, Han
Meng, Chuang
Sun, Lin
Yin, Yuelan
Chen, Xiang
Jiao, Xinan
author_sort Xu, Zhengzhong
collection PubMed
description BACKGROUND: Control of Mycobacterium tuberculosis (Mtb) infection requires CD4(+) T-cell responses and major histocompatibility complex class II (MHC II) presentation of Mtb antigens (Ags). Dendritic cells (DCs) are the most potent of the Ag-presenting cells and are central to the initiation of T-cell immune responses. Much research has indicated that DCs play an important role in anti-mycobacterial immune responses at early infection time points, but the kinetics of Ag presentation by these cells during these events are incompletely understood. RESULTS: In the present study, we evaluated in vivo dynamics of early Ag presentation by murine lymph-node (LN) DCs in response to Mycobacterium bovis bacillus Calmette–Guérin (BCG) Ag85A protein. Results showed that the early Ag-presenting activity of murine DCs induced by M. bovis BCG Ag85A protein in vivo was transient, appearing at 4 h and being barely detectable at 72 h. The transcription levels of CIITA, MHC II and the expression of MHC II molecule on the cell surface increased following BCG infection. Moreover, BCG was found to survive within the inguinal LN DC pool, representing a continuing source of mycobacterial Ag85A protein, with which LN DCs formed Ag85A peptide-MHCII complexes in vivo. CONCLUSIONS: Our results demonstrate that a decrease in Ag85A peptide production as a result of the inhibition of Ag processing to is largely responsible for the short duration of Ag presentation by LN DCs during BCG infection in vivo.
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spelling pubmed-60197972018-07-06 Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection Xu, Zhengzhong Xia, Aihong Li, Xin Zhu, Zhaocheng Shen, Yechi Jin, Shanshan Lan, Tian Xie, Yuqing Wu, Han Meng, Chuang Sun, Lin Yin, Yuelan Chen, Xiang Jiao, Xinan BMC Immunol Research Article BACKGROUND: Control of Mycobacterium tuberculosis (Mtb) infection requires CD4(+) T-cell responses and major histocompatibility complex class II (MHC II) presentation of Mtb antigens (Ags). Dendritic cells (DCs) are the most potent of the Ag-presenting cells and are central to the initiation of T-cell immune responses. Much research has indicated that DCs play an important role in anti-mycobacterial immune responses at early infection time points, but the kinetics of Ag presentation by these cells during these events are incompletely understood. RESULTS: In the present study, we evaluated in vivo dynamics of early Ag presentation by murine lymph-node (LN) DCs in response to Mycobacterium bovis bacillus Calmette–Guérin (BCG) Ag85A protein. Results showed that the early Ag-presenting activity of murine DCs induced by M. bovis BCG Ag85A protein in vivo was transient, appearing at 4 h and being barely detectable at 72 h. The transcription levels of CIITA, MHC II and the expression of MHC II molecule on the cell surface increased following BCG infection. Moreover, BCG was found to survive within the inguinal LN DC pool, representing a continuing source of mycobacterial Ag85A protein, with which LN DCs formed Ag85A peptide-MHCII complexes in vivo. CONCLUSIONS: Our results demonstrate that a decrease in Ag85A peptide production as a result of the inhibition of Ag processing to is largely responsible for the short duration of Ag presentation by LN DCs during BCG infection in vivo. BioMed Central 2018-06-25 /pmc/articles/PMC6019797/ /pubmed/29940854 http://dx.doi.org/10.1186/s12865-018-0258-8 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Xu, Zhengzhong
Xia, Aihong
Li, Xin
Zhu, Zhaocheng
Shen, Yechi
Jin, Shanshan
Lan, Tian
Xie, Yuqing
Wu, Han
Meng, Chuang
Sun, Lin
Yin, Yuelan
Chen, Xiang
Jiao, Xinan
Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection
title Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection
title_full Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection
title_fullStr Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection
title_full_unstemmed Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection
title_short Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection
title_sort rapid loss of early antigen-presenting activity of lymph node dendritic cells against ag85a protein following mycobacterium bovis bcg infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019797/
https://www.ncbi.nlm.nih.gov/pubmed/29940854
http://dx.doi.org/10.1186/s12865-018-0258-8
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