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MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1

Despite an improvement in the efficacy of chemotherapeutic agents, the outcome of patients with prostate cancer remains poor. MicroRNA (miRNA/miR)-139 expression is often downregulated in multiple types of tumor, including in prostate cancer. The aim of the present study was to investigate the inhib...

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Autores principales: Sun, Qian, Weng, Danhui, Li, Kezhen, Li, Shuang, Bai, Xiangyang, Fang, Can, Luo, Danfeng, Wu, Peng, Chen, Gang, Wei, Juncheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019920/
https://www.ncbi.nlm.nih.gov/pubmed/29963147
http://dx.doi.org/10.3892/ol.2018.8773
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author Sun, Qian
Weng, Danhui
Li, Kezhen
Li, Shuang
Bai, Xiangyang
Fang, Can
Luo, Danfeng
Wu, Peng
Chen, Gang
Wei, Juncheng
author_facet Sun, Qian
Weng, Danhui
Li, Kezhen
Li, Shuang
Bai, Xiangyang
Fang, Can
Luo, Danfeng
Wu, Peng
Chen, Gang
Wei, Juncheng
author_sort Sun, Qian
collection PubMed
description Despite an improvement in the efficacy of chemotherapeutic agents, the outcome of patients with prostate cancer remains poor. MicroRNA (miRNA/miR)-139 expression is often downregulated in multiple types of tumor, including in prostate cancer. The aim of the present study was to investigate the inhibitory effect of miR-139 on the PC-3, C4-2B and LNCaP prostate cancer cell lines. Analysis of the cell cycle of PC-3, C4-2B and LNCaP cells transfected with miR-139 revealed a significantly increased percentage of cells in the G(1) phase and a decreased percentage in the S and G(2) phases compared with those transfected with a negative control miRNA. The growth inhibitory rate of miR-139-transfected cells 24, 48 and 72 h after transfection were 32.83±2.61, 52.58±3.2 and 62.36±4.55% in PC-3 cells; 30.28±2.25, 51.74±3.27 and 60.80±3.58% in C4-2B cells; and 33.20±2.67, 51.83±3.59 and 61.79±4.85% in LNCaP cells, respectively. The present study revealed that miR-139 inhibited the proliferation of prostate cancer cells by interfering with the cell cycle. Further study into the mechanism by which this happened suggested that miR-139 reduced cyclin D1 expression and inhibited cell proliferation through targeting Notch1.
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spelling pubmed-60199202018-06-29 MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1 Sun, Qian Weng, Danhui Li, Kezhen Li, Shuang Bai, Xiangyang Fang, Can Luo, Danfeng Wu, Peng Chen, Gang Wei, Juncheng Oncol Lett Articles Despite an improvement in the efficacy of chemotherapeutic agents, the outcome of patients with prostate cancer remains poor. MicroRNA (miRNA/miR)-139 expression is often downregulated in multiple types of tumor, including in prostate cancer. The aim of the present study was to investigate the inhibitory effect of miR-139 on the PC-3, C4-2B and LNCaP prostate cancer cell lines. Analysis of the cell cycle of PC-3, C4-2B and LNCaP cells transfected with miR-139 revealed a significantly increased percentage of cells in the G(1) phase and a decreased percentage in the S and G(2) phases compared with those transfected with a negative control miRNA. The growth inhibitory rate of miR-139-transfected cells 24, 48 and 72 h after transfection were 32.83±2.61, 52.58±3.2 and 62.36±4.55% in PC-3 cells; 30.28±2.25, 51.74±3.27 and 60.80±3.58% in C4-2B cells; and 33.20±2.67, 51.83±3.59 and 61.79±4.85% in LNCaP cells, respectively. The present study revealed that miR-139 inhibited the proliferation of prostate cancer cells by interfering with the cell cycle. Further study into the mechanism by which this happened suggested that miR-139 reduced cyclin D1 expression and inhibited cell proliferation through targeting Notch1. D.A. Spandidos 2018-07 2018-05-22 /pmc/articles/PMC6019920/ /pubmed/29963147 http://dx.doi.org/10.3892/ol.2018.8773 Text en Copyright: © Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Sun, Qian
Weng, Danhui
Li, Kezhen
Li, Shuang
Bai, Xiangyang
Fang, Can
Luo, Danfeng
Wu, Peng
Chen, Gang
Wei, Juncheng
MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1
title MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1
title_full MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1
title_fullStr MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1
title_full_unstemmed MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1
title_short MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1
title_sort microrna-139-5p inhibits human prostate cancer cell proliferation by targeting notch1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019920/
https://www.ncbi.nlm.nih.gov/pubmed/29963147
http://dx.doi.org/10.3892/ol.2018.8773
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