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MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1
Despite an improvement in the efficacy of chemotherapeutic agents, the outcome of patients with prostate cancer remains poor. MicroRNA (miRNA/miR)-139 expression is often downregulated in multiple types of tumor, including in prostate cancer. The aim of the present study was to investigate the inhib...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019920/ https://www.ncbi.nlm.nih.gov/pubmed/29963147 http://dx.doi.org/10.3892/ol.2018.8773 |
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author | Sun, Qian Weng, Danhui Li, Kezhen Li, Shuang Bai, Xiangyang Fang, Can Luo, Danfeng Wu, Peng Chen, Gang Wei, Juncheng |
author_facet | Sun, Qian Weng, Danhui Li, Kezhen Li, Shuang Bai, Xiangyang Fang, Can Luo, Danfeng Wu, Peng Chen, Gang Wei, Juncheng |
author_sort | Sun, Qian |
collection | PubMed |
description | Despite an improvement in the efficacy of chemotherapeutic agents, the outcome of patients with prostate cancer remains poor. MicroRNA (miRNA/miR)-139 expression is often downregulated in multiple types of tumor, including in prostate cancer. The aim of the present study was to investigate the inhibitory effect of miR-139 on the PC-3, C4-2B and LNCaP prostate cancer cell lines. Analysis of the cell cycle of PC-3, C4-2B and LNCaP cells transfected with miR-139 revealed a significantly increased percentage of cells in the G(1) phase and a decreased percentage in the S and G(2) phases compared with those transfected with a negative control miRNA. The growth inhibitory rate of miR-139-transfected cells 24, 48 and 72 h after transfection were 32.83±2.61, 52.58±3.2 and 62.36±4.55% in PC-3 cells; 30.28±2.25, 51.74±3.27 and 60.80±3.58% in C4-2B cells; and 33.20±2.67, 51.83±3.59 and 61.79±4.85% in LNCaP cells, respectively. The present study revealed that miR-139 inhibited the proliferation of prostate cancer cells by interfering with the cell cycle. Further study into the mechanism by which this happened suggested that miR-139 reduced cyclin D1 expression and inhibited cell proliferation through targeting Notch1. |
format | Online Article Text |
id | pubmed-6019920 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-60199202018-06-29 MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1 Sun, Qian Weng, Danhui Li, Kezhen Li, Shuang Bai, Xiangyang Fang, Can Luo, Danfeng Wu, Peng Chen, Gang Wei, Juncheng Oncol Lett Articles Despite an improvement in the efficacy of chemotherapeutic agents, the outcome of patients with prostate cancer remains poor. MicroRNA (miRNA/miR)-139 expression is often downregulated in multiple types of tumor, including in prostate cancer. The aim of the present study was to investigate the inhibitory effect of miR-139 on the PC-3, C4-2B and LNCaP prostate cancer cell lines. Analysis of the cell cycle of PC-3, C4-2B and LNCaP cells transfected with miR-139 revealed a significantly increased percentage of cells in the G(1) phase and a decreased percentage in the S and G(2) phases compared with those transfected with a negative control miRNA. The growth inhibitory rate of miR-139-transfected cells 24, 48 and 72 h after transfection were 32.83±2.61, 52.58±3.2 and 62.36±4.55% in PC-3 cells; 30.28±2.25, 51.74±3.27 and 60.80±3.58% in C4-2B cells; and 33.20±2.67, 51.83±3.59 and 61.79±4.85% in LNCaP cells, respectively. The present study revealed that miR-139 inhibited the proliferation of prostate cancer cells by interfering with the cell cycle. Further study into the mechanism by which this happened suggested that miR-139 reduced cyclin D1 expression and inhibited cell proliferation through targeting Notch1. D.A. Spandidos 2018-07 2018-05-22 /pmc/articles/PMC6019920/ /pubmed/29963147 http://dx.doi.org/10.3892/ol.2018.8773 Text en Copyright: © Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Sun, Qian Weng, Danhui Li, Kezhen Li, Shuang Bai, Xiangyang Fang, Can Luo, Danfeng Wu, Peng Chen, Gang Wei, Juncheng MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1 |
title | MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1 |
title_full | MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1 |
title_fullStr | MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1 |
title_full_unstemmed | MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1 |
title_short | MicroRNA-139-5P inhibits human prostate cancer cell proliferation by targeting Notch1 |
title_sort | microrna-139-5p inhibits human prostate cancer cell proliferation by targeting notch1 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6019920/ https://www.ncbi.nlm.nih.gov/pubmed/29963147 http://dx.doi.org/10.3892/ol.2018.8773 |
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