Cargando…

Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy

BACKGROUND: To compare the abundance of vitreous proteins between the patients with proliferative diabetic retinopathy (PDR) and idiopathic macular hole (IMH). METHODS: In this study, we performed mass spectrometry-based label-free quantitative proteomics analysis of vitreous samples from type 2 dia...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Jianqing, Lu, Qianyi, Lu, Peirong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6020172/
https://www.ncbi.nlm.nih.gov/pubmed/29940965
http://dx.doi.org/10.1186/s12886-018-0821-3
_version_ 1783335238379765760
author Li, Jianqing
Lu, Qianyi
Lu, Peirong
author_facet Li, Jianqing
Lu, Qianyi
Lu, Peirong
author_sort Li, Jianqing
collection PubMed
description BACKGROUND: To compare the abundance of vitreous proteins between the patients with proliferative diabetic retinopathy (PDR) and idiopathic macular hole (IMH). METHODS: In this study, we performed mass spectrometry-based label-free quantitative proteomics analysis of vitreous samples from type 2 diabetic patients with PDR (n = 9) and IMH subjects (n = 9) and identified the abundance of 610 proteins. RESULTS: Out of 610 proteins, 64 proteins (Group A) were unique to PDR patients, while 212 proteins (Group B) could be identified in IMH vitreous only. Among the other 334 proteins that could be detected in both PDR and IMH eyes, 62 proteins differed significantly (p < 0.05, fold change > 2), which included 52 proteins (Group C) and 10 proteins (Group D) over- and under-expressed in PDR vitreous compared with the control. All proteins in these four groups were counted as significant proteins in our study. CONCLUSIONS: We identified and quantified 610 proteins in total, which included 338 significant proteins in our study. Protein distribution analysis demonstrated a clear separation of protein expression in PDR and IMH. The protein function analysis illustrated that immunity and transport related proteins might be associated with PDR.
format Online
Article
Text
id pubmed-6020172
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-60201722018-07-06 Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy Li, Jianqing Lu, Qianyi Lu, Peirong BMC Ophthalmol Research Article BACKGROUND: To compare the abundance of vitreous proteins between the patients with proliferative diabetic retinopathy (PDR) and idiopathic macular hole (IMH). METHODS: In this study, we performed mass spectrometry-based label-free quantitative proteomics analysis of vitreous samples from type 2 diabetic patients with PDR (n = 9) and IMH subjects (n = 9) and identified the abundance of 610 proteins. RESULTS: Out of 610 proteins, 64 proteins (Group A) were unique to PDR patients, while 212 proteins (Group B) could be identified in IMH vitreous only. Among the other 334 proteins that could be detected in both PDR and IMH eyes, 62 proteins differed significantly (p < 0.05, fold change > 2), which included 52 proteins (Group C) and 10 proteins (Group D) over- and under-expressed in PDR vitreous compared with the control. All proteins in these four groups were counted as significant proteins in our study. CONCLUSIONS: We identified and quantified 610 proteins in total, which included 338 significant proteins in our study. Protein distribution analysis demonstrated a clear separation of protein expression in PDR and IMH. The protein function analysis illustrated that immunity and transport related proteins might be associated with PDR. BioMed Central 2018-06-26 /pmc/articles/PMC6020172/ /pubmed/29940965 http://dx.doi.org/10.1186/s12886-018-0821-3 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Li, Jianqing
Lu, Qianyi
Lu, Peirong
Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy
title Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy
title_full Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy
title_fullStr Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy
title_full_unstemmed Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy
title_short Quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy
title_sort quantitative proteomics analysis of vitreous body from type 2 diabetic patients with proliferative diabetic retinopathy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6020172/
https://www.ncbi.nlm.nih.gov/pubmed/29940965
http://dx.doi.org/10.1186/s12886-018-0821-3
work_keys_str_mv AT lijianqing quantitativeproteomicsanalysisofvitreousbodyfromtype2diabeticpatientswithproliferativediabeticretinopathy
AT luqianyi quantitativeproteomicsanalysisofvitreousbodyfromtype2diabeticpatientswithproliferativediabeticretinopathy
AT lupeirong quantitativeproteomicsanalysisofvitreousbodyfromtype2diabeticpatientswithproliferativediabeticretinopathy