Cargando…
Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession
BACKGROUND: The green peach aphid (GPA), Myzus persicae, is economically one of the most threatening pests in pepper cultivation, which not only causes direct damage but also transmits many viruses. Breeding aphid resistant pepper varieties is a promising and environmentally friendly method to contr...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6020309/ https://www.ncbi.nlm.nih.gov/pubmed/29945550 http://dx.doi.org/10.1186/s12870-018-1340-3 |
_version_ | 1783335268005183488 |
---|---|
author | Sun, Mengjing Voorrips, Roeland E. Steenhuis-Broers, Greet van’t Westende, Wendy Vosman, Ben |
author_facet | Sun, Mengjing Voorrips, Roeland E. Steenhuis-Broers, Greet van’t Westende, Wendy Vosman, Ben |
author_sort | Sun, Mengjing |
collection | PubMed |
description | BACKGROUND: The green peach aphid (GPA), Myzus persicae, is economically one of the most threatening pests in pepper cultivation, which not only causes direct damage but also transmits many viruses. Breeding aphid resistant pepper varieties is a promising and environmentally friendly method to control aphid populations in the field and in the greenhouse. Until now, no strong sources of resistance against the GPA have been identified. Therefore the main aims of this study were to identify pepper materials with a good level of resistance to GPA and to elucidate possible resistance mechanisms. RESULTS: We screened 74 pepper accessions from different geographical areas for resistance to M. persicae. After four rounds of evaluation we identified one Capsicum baccatum accession (PB2013071) as highly resistant to M. persicae, while the accessions PB2013062 and PB2012022 showed intermediate resistance. The resistance of PB2013071 resulted in a severely reduced uptake of phloem compared to the susceptible accession, as determined by Electrical Penetration Graph (EPG) studies. Feeding of M. persicae induced the expression of callose synthase genes and resulted in callose deposition in the sieve elements in resistant, but not in susceptible plants. CONCLUSIONS: Three aphid resistant pepper accessions were identified, which will be important for breeding aphid resistant pepper varieties in the future. The most resistant accession PB2013071 showed phloem-based resistance against aphid infestation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1340-3) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6020309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-60203092018-07-06 Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession Sun, Mengjing Voorrips, Roeland E. Steenhuis-Broers, Greet van’t Westende, Wendy Vosman, Ben BMC Plant Biol Research Article BACKGROUND: The green peach aphid (GPA), Myzus persicae, is economically one of the most threatening pests in pepper cultivation, which not only causes direct damage but also transmits many viruses. Breeding aphid resistant pepper varieties is a promising and environmentally friendly method to control aphid populations in the field and in the greenhouse. Until now, no strong sources of resistance against the GPA have been identified. Therefore the main aims of this study were to identify pepper materials with a good level of resistance to GPA and to elucidate possible resistance mechanisms. RESULTS: We screened 74 pepper accessions from different geographical areas for resistance to M. persicae. After four rounds of evaluation we identified one Capsicum baccatum accession (PB2013071) as highly resistant to M. persicae, while the accessions PB2013062 and PB2012022 showed intermediate resistance. The resistance of PB2013071 resulted in a severely reduced uptake of phloem compared to the susceptible accession, as determined by Electrical Penetration Graph (EPG) studies. Feeding of M. persicae induced the expression of callose synthase genes and resulted in callose deposition in the sieve elements in resistant, but not in susceptible plants. CONCLUSIONS: Three aphid resistant pepper accessions were identified, which will be important for breeding aphid resistant pepper varieties in the future. The most resistant accession PB2013071 showed phloem-based resistance against aphid infestation. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12870-018-1340-3) contains supplementary material, which is available to authorized users. BioMed Central 2018-06-27 /pmc/articles/PMC6020309/ /pubmed/29945550 http://dx.doi.org/10.1186/s12870-018-1340-3 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Sun, Mengjing Voorrips, Roeland E. Steenhuis-Broers, Greet van’t Westende, Wendy Vosman, Ben Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession |
title | Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession |
title_full | Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession |
title_fullStr | Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession |
title_full_unstemmed | Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession |
title_short | Reduced phloem uptake of Myzus persicae on an aphid resistant pepper accession |
title_sort | reduced phloem uptake of myzus persicae on an aphid resistant pepper accession |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6020309/ https://www.ncbi.nlm.nih.gov/pubmed/29945550 http://dx.doi.org/10.1186/s12870-018-1340-3 |
work_keys_str_mv | AT sunmengjing reducedphloemuptakeofmyzuspersicaeonanaphidresistantpepperaccession AT voorripsroelande reducedphloemuptakeofmyzuspersicaeonanaphidresistantpepperaccession AT steenhuisbroersgreet reducedphloemuptakeofmyzuspersicaeonanaphidresistantpepperaccession AT vantwestendewendy reducedphloemuptakeofmyzuspersicaeonanaphidresistantpepperaccession AT vosmanben reducedphloemuptakeofmyzuspersicaeonanaphidresistantpepperaccession |