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Decreased expression of TRPV1 in renal cell carcinoma: association with tumor Fuhrman grades and histopathological subtypes
PURPOSE: The aim of this study was to investigate whether the expression of the ligand-gated Ca(2+) channel transient receptor potential vanilloid type-1 (TRPV1) in primary human renal cell carcinoma (RCC) is associated with clinicopathological features. PATIENTS AND METHODS: Fresh and frozen primar...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021007/ https://www.ncbi.nlm.nih.gov/pubmed/29970964 http://dx.doi.org/10.2147/CMAR.S166390 |
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author | Wu, Yong-Yang Liu, Xin-Yu Zhuo, De-Xiang Huang, Huai-Bin Zhang, Fa-Biao Liao, Shang-Fan |
author_facet | Wu, Yong-Yang Liu, Xin-Yu Zhuo, De-Xiang Huang, Huai-Bin Zhang, Fa-Biao Liao, Shang-Fan |
author_sort | Wu, Yong-Yang |
collection | PubMed |
description | PURPOSE: The aim of this study was to investigate whether the expression of the ligand-gated Ca(2+) channel transient receptor potential vanilloid type-1 (TRPV1) in primary human renal cell carcinoma (RCC) is associated with clinicopathological features. PATIENTS AND METHODS: Fresh and frozen primary tumor and normal peritumoral kidney tissues from 127 patients diagnosed with RCC were analyzed for TRPV1 expression by quantitative reverse transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry. RESULTS: Quantitative RT-PCR revealed that TRPV1 was decreased 3.20-fold in RCC tissue vs normal peritumoral kidney tissue (p=0.012). Significantly different TRPV1 mRNA expression was detected in RCC tissues of different Fuhrman grades and histopathological subtypes (F=4.282, p=0.015 and F=5.205, p=0.014, respectively). Decreased TRPV1 expression was correlated with RCC histopathological subtype (R=-0.554, p=0.003) and Fuhrman grade (R=−0.525, p=0.006). Western blot analysis of TRPV1 protein expression showed similar results. Immunohistochemical analysis showed strong expression of TRPV1 in kidney tubules but demonstrated weak or no immunostaining in RCC tissues. CONCLUSION: TRPV1 expression was decreased in RCC, which was significantly associated with tumor Fuhrman grades and histopathological subtypes. It seems to suggest that TRPV1 expression may be a valuable tool to predict the extent of RCC progression. |
format | Online Article Text |
id | pubmed-6021007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-60210072018-07-03 Decreased expression of TRPV1 in renal cell carcinoma: association with tumor Fuhrman grades and histopathological subtypes Wu, Yong-Yang Liu, Xin-Yu Zhuo, De-Xiang Huang, Huai-Bin Zhang, Fa-Biao Liao, Shang-Fan Cancer Manag Res Original Research PURPOSE: The aim of this study was to investigate whether the expression of the ligand-gated Ca(2+) channel transient receptor potential vanilloid type-1 (TRPV1) in primary human renal cell carcinoma (RCC) is associated with clinicopathological features. PATIENTS AND METHODS: Fresh and frozen primary tumor and normal peritumoral kidney tissues from 127 patients diagnosed with RCC were analyzed for TRPV1 expression by quantitative reverse transcription polymerase chain reaction (RT-PCR), Western blotting and immunohistochemistry. RESULTS: Quantitative RT-PCR revealed that TRPV1 was decreased 3.20-fold in RCC tissue vs normal peritumoral kidney tissue (p=0.012). Significantly different TRPV1 mRNA expression was detected in RCC tissues of different Fuhrman grades and histopathological subtypes (F=4.282, p=0.015 and F=5.205, p=0.014, respectively). Decreased TRPV1 expression was correlated with RCC histopathological subtype (R=-0.554, p=0.003) and Fuhrman grade (R=−0.525, p=0.006). Western blot analysis of TRPV1 protein expression showed similar results. Immunohistochemical analysis showed strong expression of TRPV1 in kidney tubules but demonstrated weak or no immunostaining in RCC tissues. CONCLUSION: TRPV1 expression was decreased in RCC, which was significantly associated with tumor Fuhrman grades and histopathological subtypes. It seems to suggest that TRPV1 expression may be a valuable tool to predict the extent of RCC progression. Dove Medical Press 2018-06-22 /pmc/articles/PMC6021007/ /pubmed/29970964 http://dx.doi.org/10.2147/CMAR.S166390 Text en © 2018 Wu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Wu, Yong-Yang Liu, Xin-Yu Zhuo, De-Xiang Huang, Huai-Bin Zhang, Fa-Biao Liao, Shang-Fan Decreased expression of TRPV1 in renal cell carcinoma: association with tumor Fuhrman grades and histopathological subtypes |
title | Decreased expression of TRPV1 in renal cell carcinoma: association with tumor Fuhrman grades and histopathological subtypes |
title_full | Decreased expression of TRPV1 in renal cell carcinoma: association with tumor Fuhrman grades and histopathological subtypes |
title_fullStr | Decreased expression of TRPV1 in renal cell carcinoma: association with tumor Fuhrman grades and histopathological subtypes |
title_full_unstemmed | Decreased expression of TRPV1 in renal cell carcinoma: association with tumor Fuhrman grades and histopathological subtypes |
title_short | Decreased expression of TRPV1 in renal cell carcinoma: association with tumor Fuhrman grades and histopathological subtypes |
title_sort | decreased expression of trpv1 in renal cell carcinoma: association with tumor fuhrman grades and histopathological subtypes |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021007/ https://www.ncbi.nlm.nih.gov/pubmed/29970964 http://dx.doi.org/10.2147/CMAR.S166390 |
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