Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing

Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyot...

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Autores principales: Xu, An, Zhou, Ruoji, Tu, Jian, Huo, Zijun, Zhu, Dandan, Wang, Donghui, Gingold, Julian A., Mata, Helen, Rao, Pulivarthi H., Liu, Mo, Mohamed, Alaa M.T., Kong, Celine Shuet Lin, Jewell, Brittany E., Xia, Weiya, Zhao, Ruiying, Hung, Mien-Chie, Lee, Dung-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021017/
https://www.ncbi.nlm.nih.gov/pubmed/29730572
http://dx.doi.org/10.1016/j.scr.2018.04.013
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author Xu, An
Zhou, Ruoji
Tu, Jian
Huo, Zijun
Zhu, Dandan
Wang, Donghui
Gingold, Julian A.
Mata, Helen
Rao, Pulivarthi H.
Liu, Mo
Mohamed, Alaa M.T.
Kong, Celine Shuet Lin
Jewell, Brittany E.
Xia, Weiya
Zhao, Ruiying
Hung, Mien-Chie
Lee, Dung-Fang
author_facet Xu, An
Zhou, Ruoji
Tu, Jian
Huo, Zijun
Zhu, Dandan
Wang, Donghui
Gingold, Julian A.
Mata, Helen
Rao, Pulivarthi H.
Liu, Mo
Mohamed, Alaa M.T.
Kong, Celine Shuet Lin
Jewell, Brittany E.
Xia, Weiya
Zhao, Ruiying
Hung, Mien-Chie
Lee, Dung-Fang
author_sort Xu, An
collection PubMed
description Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers.
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spelling pubmed-60210172018-06-27 Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing Xu, An Zhou, Ruoji Tu, Jian Huo, Zijun Zhu, Dandan Wang, Donghui Gingold, Julian A. Mata, Helen Rao, Pulivarthi H. Liu, Mo Mohamed, Alaa M.T. Kong, Celine Shuet Lin Jewell, Brittany E. Xia, Weiya Zhao, Ruiying Hung, Mien-Chie Lee, Dung-Fang Stem Cell Res Article Genetic mutations in TP53 contribute to multiple human cancers. Here we report the generation of a H1-p53(R248W/R248W) human embryonic stem cell line harboring a homozygous TP53 R248W mutation created by TALEN-mediated precise gene editing. The H1-p53(R248W/R248W) cell line maintains a normal karyotype, robust pluripotency gene expression, and the potential to differentiate to the three germ layers. 2018-04-27 2018-05 /pmc/articles/PMC6021017/ /pubmed/29730572 http://dx.doi.org/10.1016/j.scr.2018.04.013 Text en https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Article
Xu, An
Zhou, Ruoji
Tu, Jian
Huo, Zijun
Zhu, Dandan
Wang, Donghui
Gingold, Julian A.
Mata, Helen
Rao, Pulivarthi H.
Liu, Mo
Mohamed, Alaa M.T.
Kong, Celine Shuet Lin
Jewell, Brittany E.
Xia, Weiya
Zhao, Ruiying
Hung, Mien-Chie
Lee, Dung-Fang
Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing
title Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing
title_full Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing
title_fullStr Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing
title_full_unstemmed Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing
title_short Establishment of a human embryonic stem cell line with homozygous TP53 R248W mutant by TALEN mediated gene editing
title_sort establishment of a human embryonic stem cell line with homozygous tp53 r248w mutant by talen mediated gene editing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021017/
https://www.ncbi.nlm.nih.gov/pubmed/29730572
http://dx.doi.org/10.1016/j.scr.2018.04.013
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