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Seamless assembly of recombinant adenoviral genomes from high-copy plasmids

The adenoviruses are essential tools for basic research and therapeutic development. Robust methods for the generation of mutant and recombinant viruses are crucial for these diverse applications. Here we describe a simple plasmid-based method that permits highly efficient modification of the adenov...

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Detalles Bibliográficos
Autores principales: Miciak, Jessica J., Hirshberg, Jason, Bunz, Fred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021080/
https://www.ncbi.nlm.nih.gov/pubmed/29949649
http://dx.doi.org/10.1371/journal.pone.0199563