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Seamless assembly of recombinant adenoviral genomes from high-copy plasmids
The adenoviruses are essential tools for basic research and therapeutic development. Robust methods for the generation of mutant and recombinant viruses are crucial for these diverse applications. Here we describe a simple plasmid-based method that permits highly efficient modification of the adenov...
Autores principales: | Miciak, Jessica J., Hirshberg, Jason, Bunz, Fred |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021080/ https://www.ncbi.nlm.nih.gov/pubmed/29949649 http://dx.doi.org/10.1371/journal.pone.0199563 |
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