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Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer
BACKGROUND: Differentially expressed genes and their post-transcriptional regulator-microRNAs (miRNAs), are potential keys to pioneering cancer diagnosis and treatment. The aim of this study was to investigate how the miRNA-mRNA interactions might affect the tumorigenesis of bladder cancer (BC) and...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021253/ https://www.ncbi.nlm.nih.gov/pubmed/29963227 http://dx.doi.org/10.18632/oncotarget.24441 |
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author | Lee, Jong-Young Yun, Seok Joong Jeong, Pildu Piao, Xuan-Mei Kim, Ye-Hwan Kim, Jihye Subramaniyam, Sathiyamoorthy Byun, Young Joon Kang, Ho Won Seo, Sung Phil Kim, Jayoung Kim, Jung Min Yoo, Eun Sang Kim, Isaac Y. Moon, Sung-Kwon Choi, Yung Hyun Kim, Wun-Jae |
author_facet | Lee, Jong-Young Yun, Seok Joong Jeong, Pildu Piao, Xuan-Mei Kim, Ye-Hwan Kim, Jihye Subramaniyam, Sathiyamoorthy Byun, Young Joon Kang, Ho Won Seo, Sung Phil Kim, Jayoung Kim, Jung Min Yoo, Eun Sang Kim, Isaac Y. Moon, Sung-Kwon Choi, Yung Hyun Kim, Wun-Jae |
author_sort | Lee, Jong-Young |
collection | PubMed |
description | BACKGROUND: Differentially expressed genes and their post-transcriptional regulator-microRNAs (miRNAs), are potential keys to pioneering cancer diagnosis and treatment. The aim of this study was to investigate how the miRNA-mRNA interactions might affect the tumorigenesis of bladder cancer (BC) and to identify specific miRNA and mRNA genetic markers in the two BC types: non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC). RESULTS: We identified 227 genes that interacted with 54 miRNAs in NMIBC, and 14 genes that interacted with 10 miRNAs in MIBC. Based on this data, we found extracellular matrix-related genes are highly enriched in NMIBC while genes related to core nuclear division are highly enriched in MIBC. Furthermore, using a transcriptional regulatory element database, we found indirect regulatory transcription factors (TFs) for enriched genes could regulate tumorigenesis with or without miRNAs. MATERIALS AND METHODS: Tissue samples from 234 patients histologically diagnosed with BC and 83 individuals without BC were analyzed using microarray and next-generation sequencing technology, and we used different cut-offs to identify differentially expressed mRNAs and miRNAs in NMIBC and MIBC. The selected mRNAs and miRNAs were paired using validated target datasets and according to inverse expression relationships. MiRNA interacted genes were compared with the TF-regulating genes in BC. Meanwhile, pathway enrichment analysis was performed to identify the functions of selected miRNAs and genes. CONCLUSIONS: Identification of differential gene expression in specific tumor types could facilitate development of cancer diagnosis and aid in the early detection of BC. |
format | Online Article Text |
id | pubmed-6021253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-60212532018-06-29 Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer Lee, Jong-Young Yun, Seok Joong Jeong, Pildu Piao, Xuan-Mei Kim, Ye-Hwan Kim, Jihye Subramaniyam, Sathiyamoorthy Byun, Young Joon Kang, Ho Won Seo, Sung Phil Kim, Jayoung Kim, Jung Min Yoo, Eun Sang Kim, Isaac Y. Moon, Sung-Kwon Choi, Yung Hyun Kim, Wun-Jae Oncotarget Research Paper BACKGROUND: Differentially expressed genes and their post-transcriptional regulator-microRNAs (miRNAs), are potential keys to pioneering cancer diagnosis and treatment. The aim of this study was to investigate how the miRNA-mRNA interactions might affect the tumorigenesis of bladder cancer (BC) and to identify specific miRNA and mRNA genetic markers in the two BC types: non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC). RESULTS: We identified 227 genes that interacted with 54 miRNAs in NMIBC, and 14 genes that interacted with 10 miRNAs in MIBC. Based on this data, we found extracellular matrix-related genes are highly enriched in NMIBC while genes related to core nuclear division are highly enriched in MIBC. Furthermore, using a transcriptional regulatory element database, we found indirect regulatory transcription factors (TFs) for enriched genes could regulate tumorigenesis with or without miRNAs. MATERIALS AND METHODS: Tissue samples from 234 patients histologically diagnosed with BC and 83 individuals without BC were analyzed using microarray and next-generation sequencing technology, and we used different cut-offs to identify differentially expressed mRNAs and miRNAs in NMIBC and MIBC. The selected mRNAs and miRNAs were paired using validated target datasets and according to inverse expression relationships. MiRNA interacted genes were compared with the TF-regulating genes in BC. Meanwhile, pathway enrichment analysis was performed to identify the functions of selected miRNAs and genes. CONCLUSIONS: Identification of differential gene expression in specific tumor types could facilitate development of cancer diagnosis and aid in the early detection of BC. Impact Journals LLC 2018-02-07 /pmc/articles/PMC6021253/ /pubmed/29963227 http://dx.doi.org/10.18632/oncotarget.24441 Text en Copyright: © 2018 Lee et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Lee, Jong-Young Yun, Seok Joong Jeong, Pildu Piao, Xuan-Mei Kim, Ye-Hwan Kim, Jihye Subramaniyam, Sathiyamoorthy Byun, Young Joon Kang, Ho Won Seo, Sung Phil Kim, Jayoung Kim, Jung Min Yoo, Eun Sang Kim, Isaac Y. Moon, Sung-Kwon Choi, Yung Hyun Kim, Wun-Jae Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer |
title | Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer |
title_full | Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer |
title_fullStr | Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer |
title_full_unstemmed | Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer |
title_short | Identification of differentially expressed miRNAs and miRNA-targeted genes in bladder cancer |
title_sort | identification of differentially expressed mirnas and mirna-targeted genes in bladder cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021253/ https://www.ncbi.nlm.nih.gov/pubmed/29963227 http://dx.doi.org/10.18632/oncotarget.24441 |
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