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Effect of 8‐week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats

We investigated the effects of regular leucine intake and/or resistance exercise training on skeletal muscle hypertrophy and satellite cell activity after the administration of different doses of leucine. Ten‐week‐old Sprague–Dawley rats were assigned to six groups (n = 7 per group): a control group...

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Autores principales: Lim, Chang Hyun, Gil, Ju Hyun, Quan, Helong, Viet, Dang Ha, Kim, Chang Keun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021278/
https://www.ncbi.nlm.nih.gov/pubmed/29952091
http://dx.doi.org/10.14814/phy2.13725
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author Lim, Chang Hyun
Gil, Ju Hyun
Quan, Helong
Viet, Dang Ha
Kim, Chang Keun
author_facet Lim, Chang Hyun
Gil, Ju Hyun
Quan, Helong
Viet, Dang Ha
Kim, Chang Keun
author_sort Lim, Chang Hyun
collection PubMed
description We investigated the effects of regular leucine intake and/or resistance exercise training on skeletal muscle hypertrophy and satellite cell activity after the administration of different doses of leucine. Ten‐week‐old Sprague–Dawley rats were assigned to six groups (n = 7 per group): a control group (Con), two groups receiving either 10% (0.135 g/kg.wt) (Leu10) or 50% (0.675 g/kg.wt) (Leu50) leucine supplementation, and three exercise groups receiving 0% (Ex), 10% (Leu10Ex), and 50% (Leu50Ex) leucine supplementation. The rats performed ladder climbing exercises thrice per week for 8 weeks, and received leucine supplements at the same time daily. Muscle phenotypes were assessed by immunohistochemistry. MyoD, myogenin, and IGF1 protein levels were determined by western blot. The Leu50Ex group displayed significantly higher numbers of positive embryonic myosin fibers (0.35 ± 0.08, 250%) and myonuclei (3.29 ± 0.3, 118.7%) than all other groups. And exercise training groups increased the cross‐sectional area, the number of satellite cells and protein expression of MyoD, myogenin, and IGF1alpha relative to the Control group (P < 0.05). However, Only leucine supplementation group did not increase skeletal muscle hypertrophy and satellite cell activity, regardless of the dose (P > 0.05). Leucine intake accompanied by regular exercise training may increase satellite cell activation in skeletal muscles, and improve muscle quality more effectively than continuous leucine ingestion alone.
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spelling pubmed-60212782018-06-29 Effect of 8‐week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats Lim, Chang Hyun Gil, Ju Hyun Quan, Helong Viet, Dang Ha Kim, Chang Keun Physiol Rep Original Research We investigated the effects of regular leucine intake and/or resistance exercise training on skeletal muscle hypertrophy and satellite cell activity after the administration of different doses of leucine. Ten‐week‐old Sprague–Dawley rats were assigned to six groups (n = 7 per group): a control group (Con), two groups receiving either 10% (0.135 g/kg.wt) (Leu10) or 50% (0.675 g/kg.wt) (Leu50) leucine supplementation, and three exercise groups receiving 0% (Ex), 10% (Leu10Ex), and 50% (Leu50Ex) leucine supplementation. The rats performed ladder climbing exercises thrice per week for 8 weeks, and received leucine supplements at the same time daily. Muscle phenotypes were assessed by immunohistochemistry. MyoD, myogenin, and IGF1 protein levels were determined by western blot. The Leu50Ex group displayed significantly higher numbers of positive embryonic myosin fibers (0.35 ± 0.08, 250%) and myonuclei (3.29 ± 0.3, 118.7%) than all other groups. And exercise training groups increased the cross‐sectional area, the number of satellite cells and protein expression of MyoD, myogenin, and IGF1alpha relative to the Control group (P < 0.05). However, Only leucine supplementation group did not increase skeletal muscle hypertrophy and satellite cell activity, regardless of the dose (P > 0.05). Leucine intake accompanied by regular exercise training may increase satellite cell activation in skeletal muscles, and improve muscle quality more effectively than continuous leucine ingestion alone. John Wiley and Sons Inc. 2018-06-27 /pmc/articles/PMC6021278/ /pubmed/29952091 http://dx.doi.org/10.14814/phy2.13725 Text en © 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Lim, Chang Hyun
Gil, Ju Hyun
Quan, Helong
Viet, Dang Ha
Kim, Chang Keun
Effect of 8‐week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats
title Effect of 8‐week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats
title_full Effect of 8‐week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats
title_fullStr Effect of 8‐week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats
title_full_unstemmed Effect of 8‐week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats
title_short Effect of 8‐week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats
title_sort effect of 8‐week leucine supplementation and resistance exercise training on muscle hypertrophy and satellite cell activation in rats
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021278/
https://www.ncbi.nlm.nih.gov/pubmed/29952091
http://dx.doi.org/10.14814/phy2.13725
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