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Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle

The administration of follicle-stimulating hormone (FSH) prior to oocyte retrieval improves oocyte developmental competence. During bovine embryo production in vitro, however, oocytes are typically derived from FSH-unprimed animals. In the current study, we examined the effect of pre-in vitro matura...

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Autores principales: SUGIMURA, Satoshi, YAMANOUCHI, Tadayuki, PALMERINI, Maria Grazia, HASHIYADA, Yutaka, IMAI, Kei, GILCHRIST, Robert B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021610/
https://www.ncbi.nlm.nih.gov/pubmed/29503399
http://dx.doi.org/10.1262/jrd.2018-009
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author SUGIMURA, Satoshi
YAMANOUCHI, Tadayuki
PALMERINI, Maria Grazia
HASHIYADA, Yutaka
IMAI, Kei
GILCHRIST, Robert B
author_facet SUGIMURA, Satoshi
YAMANOUCHI, Tadayuki
PALMERINI, Maria Grazia
HASHIYADA, Yutaka
IMAI, Kei
GILCHRIST, Robert B
author_sort SUGIMURA, Satoshi
collection PubMed
description The administration of follicle-stimulating hormone (FSH) prior to oocyte retrieval improves oocyte developmental competence. During bovine embryo production in vitro, however, oocytes are typically derived from FSH-unprimed animals. In the current study, we examined the effect of pre-in vitro maturation (IVM) with cAMP modulators, also known as the second messengers of FSH, on the developmental competence of oocytes derived from small antral follicles (2–4 mm) of FSH-unprimed animals. Pre-IVM with N6,2ʹ-O-dibutyryladenosine 3′,5′-cyclicmonophosphate (dbcAMP) and 3-isobutyl-1-methylxanthine (IBMX) for 2 h improved the blastocyst formation in oocytes stimulated by FSH or amphiregulin (AREG). Furthermore, pre-IVM enhanced the expression of the FSH- or AREG-stimulated extracellular matrix-related genes HAS2, TNFAIP6, and PTGS2, and epidermal growth factor (EGF)-like peptide-related genes AREG and EREG. Additionally, pre-IVM with dbcAMP and IBMX enhanced the expression of EGFR, and also increased and prolonged cumulus cell-oocyte gap junctional communication. The improved oocyte development observed using the pre-IVM protocol was ablated by an EGF receptor phosphorylation inhibitor. These results indicate that pre-IVM with cAMP modulators could contribute to the acquisition of developmental competence by bovine oocytes from small antral follicles through the modulation of EGF receptor signaling and oocyte-cumulus/cumulus-cumulus gap junctional communication.
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spelling pubmed-60216102018-07-05 Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle SUGIMURA, Satoshi YAMANOUCHI, Tadayuki PALMERINI, Maria Grazia HASHIYADA, Yutaka IMAI, Kei GILCHRIST, Robert B J Reprod Dev Original Article The administration of follicle-stimulating hormone (FSH) prior to oocyte retrieval improves oocyte developmental competence. During bovine embryo production in vitro, however, oocytes are typically derived from FSH-unprimed animals. In the current study, we examined the effect of pre-in vitro maturation (IVM) with cAMP modulators, also known as the second messengers of FSH, on the developmental competence of oocytes derived from small antral follicles (2–4 mm) of FSH-unprimed animals. Pre-IVM with N6,2ʹ-O-dibutyryladenosine 3′,5′-cyclicmonophosphate (dbcAMP) and 3-isobutyl-1-methylxanthine (IBMX) for 2 h improved the blastocyst formation in oocytes stimulated by FSH or amphiregulin (AREG). Furthermore, pre-IVM enhanced the expression of the FSH- or AREG-stimulated extracellular matrix-related genes HAS2, TNFAIP6, and PTGS2, and epidermal growth factor (EGF)-like peptide-related genes AREG and EREG. Additionally, pre-IVM with dbcAMP and IBMX enhanced the expression of EGFR, and also increased and prolonged cumulus cell-oocyte gap junctional communication. The improved oocyte development observed using the pre-IVM protocol was ablated by an EGF receptor phosphorylation inhibitor. These results indicate that pre-IVM with cAMP modulators could contribute to the acquisition of developmental competence by bovine oocytes from small antral follicles through the modulation of EGF receptor signaling and oocyte-cumulus/cumulus-cumulus gap junctional communication. The Society for Reproduction and Development 2018-03-02 2018-06 /pmc/articles/PMC6021610/ /pubmed/29503399 http://dx.doi.org/10.1262/jrd.2018-009 Text en ©2018 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Original Article
SUGIMURA, Satoshi
YAMANOUCHI, Tadayuki
PALMERINI, Maria Grazia
HASHIYADA, Yutaka
IMAI, Kei
GILCHRIST, Robert B
Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle
title Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle
title_full Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle
title_fullStr Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle
title_full_unstemmed Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle
title_short Effect of pre-in vitro maturation with cAMP modulators on the acquisition of oocyte developmental competence in cattle
title_sort effect of pre-in vitro maturation with camp modulators on the acquisition of oocyte developmental competence in cattle
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021610/
https://www.ncbi.nlm.nih.gov/pubmed/29503399
http://dx.doi.org/10.1262/jrd.2018-009
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