Retained Activity of an O25b-Specific Monoclonal Antibody against an Mcr-1-Producing Escherichia coli Sequence Type 131 Strain

Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic Escherichia coli strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxin...

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Autores principales: Guachalla, Luis M., Ramoni, Katharina, Varga, Cecilia, Mutti, Michele, Ghazawi, Akela, Pál, Tibor, Nagy, Eszter, Sonnevend, Ágnes, Nagy, Gábor, Szijártó, Valéria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Experimental Therapeutics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021686/
https://www.ncbi.nlm.nih.gov/pubmed/29686149
http://dx.doi.org/10.1128/AAC.00046-18
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author Guachalla, Luis M.
Ramoni, Katharina
Varga, Cecilia
Mutti, Michele
Ghazawi, Akela
Pál, Tibor
Nagy, Eszter
Sonnevend, Ágnes
Nagy, Gábor
Szijártó, Valéria
author_facet Guachalla, Luis M.
Ramoni, Katharina
Varga, Cecilia
Mutti, Michele
Ghazawi, Akela
Pál, Tibor
Nagy, Eszter
Sonnevend, Ágnes
Nagy, Gábor
Szijártó, Valéria
author_sort Guachalla, Luis M.
collection PubMed
description Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic Escherichia coli strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody ASN-4, specific to the O25b side chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin-neutralizing activities and therefore offers a treatment option against extremely drug-resistant ST131 isolates.
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spelling pubmed-60216862018-07-06 Retained Activity of an O25b-Specific Monoclonal Antibody against an Mcr-1-Producing Escherichia coli Sequence Type 131 Strain Guachalla, Luis M. Ramoni, Katharina Varga, Cecilia Mutti, Michele Ghazawi, Akela Pál, Tibor Nagy, Eszter Sonnevend, Ágnes Nagy, Gábor Szijártó, Valéria Antimicrob Agents Chemother Experimental Therapeutics Plasmid-encoded colistin resistance is emerging among extraintestinal pathogenic Escherichia coli strains, including those of the epidemic clone sequence type 131 (ST131)-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of lipopolysaccharide (LPS), conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody ASN-4, specific to the O25b side chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin-neutralizing activities and therefore offers a treatment option against extremely drug-resistant ST131 isolates. American Society for Microbiology 2018-06-26 /pmc/articles/PMC6021686/ /pubmed/29686149 http://dx.doi.org/10.1128/AAC.00046-18 Text en Copyright © 2018 Guachalla et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Experimental Therapeutics
Guachalla, Luis M.
Ramoni, Katharina
Varga, Cecilia
Mutti, Michele
Ghazawi, Akela
Pál, Tibor
Nagy, Eszter
Sonnevend, Ágnes
Nagy, Gábor
Szijártó, Valéria
Retained Activity of an O25b-Specific Monoclonal Antibody against an Mcr-1-Producing Escherichia coli Sequence Type 131 Strain
title Retained Activity of an O25b-Specific Monoclonal Antibody against an Mcr-1-Producing Escherichia coli Sequence Type 131 Strain
title_full Retained Activity of an O25b-Specific Monoclonal Antibody against an Mcr-1-Producing Escherichia coli Sequence Type 131 Strain
title_fullStr Retained Activity of an O25b-Specific Monoclonal Antibody against an Mcr-1-Producing Escherichia coli Sequence Type 131 Strain
title_full_unstemmed Retained Activity of an O25b-Specific Monoclonal Antibody against an Mcr-1-Producing Escherichia coli Sequence Type 131 Strain
title_short Retained Activity of an O25b-Specific Monoclonal Antibody against an Mcr-1-Producing Escherichia coli Sequence Type 131 Strain
title_sort retained activity of an o25b-specific monoclonal antibody against an mcr-1-producing escherichia coli sequence type 131 strain
topic Experimental Therapeutics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6021686/
https://www.ncbi.nlm.nih.gov/pubmed/29686149
http://dx.doi.org/10.1128/AAC.00046-18
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