肺腺癌长链非编码RNA启动子DNA甲基化的生物信息学分析

BACKGROUND AND OBJECTIVE: Previous studies have found the lincRNAs play important roles in the occurrence and development of lung adenocarcinoma, but their regulated mechanims are still unclear.The aim of this study is to evaluate the relationship between DNA methylation and lincRNA expression, and the effect of prognosis in lung adenocarcinoma. METHODS: The whole genome Illumina Methylation 450 DNA methylatiuon data and RNAseq for lung adenocarcinoma were download from TCGA.DNA methylation around lincRNA and their relationship to gene expression were analyzed.Their contribution to lung adenocarcinoma were further analyzed by comparing DNA methylation and lincRNA expression in tumor and adjacent tissues. RESULTS: The methylation level in promoter region was lower than other positions in lincRNA, and was negatively correlated with gene expression.There were 427 lincRNA genes showed differential DNA methylation in their promoter regions in tumor and adjacent tissues.Among these, 15 lincRNA genes showed differential gene expression and negatively correlated with DNA methylation, including FENDRR (a tumor progression and metastasis gene) whose high methylation in its promoter showed poor survival in lung adenocarcinoma. CONCLUSION: The expression of lincRNA can be regulated by DNA methylation in their promoter regions, and the level of DNA methylation is related to patient prognosis in lung adenocarcinoma.