Cargando…
Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40
Determination of the conformation (monomer, oligomer, or fibril) of amyloid peptide aggregates in the human brain is essential for the diagnosis and treatment of Alzheimer’s disease (AD). Accordingly, systematic investigation of amyloid conformation using analytical tools is essential for precisely...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022081/ https://www.ncbi.nlm.nih.gov/pubmed/29843431 http://dx.doi.org/10.3390/s18061738 |
_version_ | 1783335602048991232 |
---|---|
author | Jeong, Dahye Kim, Jinsik Chae, Myung-Sic Lee, Wonseok Yang, Seung-Hoon Kim, YoungSoo Kim, Seung Min Lee, Jin San Lee, Jeong Hoon Choi, Jungkyu Yoon, Dae Sung Hwang, Kyo Seon |
author_facet | Jeong, Dahye Kim, Jinsik Chae, Myung-Sic Lee, Wonseok Yang, Seung-Hoon Kim, YoungSoo Kim, Seung Min Lee, Jin San Lee, Jeong Hoon Choi, Jungkyu Yoon, Dae Sung Hwang, Kyo Seon |
author_sort | Jeong, Dahye |
collection | PubMed |
description | Determination of the conformation (monomer, oligomer, or fibril) of amyloid peptide aggregates in the human brain is essential for the diagnosis and treatment of Alzheimer’s disease (AD). Accordingly, systematic investigation of amyloid conformation using analytical tools is essential for precisely quantifying the relative amounts of the three conformations of amyloid peptide. Here, we developed a reduced graphene oxide (rGO) based multiplexing biosensor that could be used to monitor the relative amounts of the three conformations of various amyloid-β 40 (Aβ40) fluids. The electrical rGO biosensor was composed of a multichannel sensor array capable of individual detection of monomers, oligomers, and fibrils in a single amyloid fluid sample. From the performance test of each sensor, we showed that this method had good analytical sensitivity (1 pg/mL) and a fairly wide dynamic range (1 pg/mL to 10 ng/mL) for each conformation of Aβ40. To verify whether the rGO biosensor could be used to evaluate the relative amounts of the three conformations, various amyloid solutions (monomeric Aβ40, aggregated Aβ40, and disaggregated Aβ40 solutions) were employed. Notably, different trends in the relative amounts of the three conformations were observed in each amyloid solution, indicating that this information could serve as an important parameter in the clinical setting. Accordingly, our analytical tool could precisely detect the relative amounts of the three conformations of Aβ40 and may have potential applications as a diagnostic system for AD. |
format | Online Article Text |
id | pubmed-6022081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60220812018-07-02 Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40 Jeong, Dahye Kim, Jinsik Chae, Myung-Sic Lee, Wonseok Yang, Seung-Hoon Kim, YoungSoo Kim, Seung Min Lee, Jin San Lee, Jeong Hoon Choi, Jungkyu Yoon, Dae Sung Hwang, Kyo Seon Sensors (Basel) Article Determination of the conformation (monomer, oligomer, or fibril) of amyloid peptide aggregates in the human brain is essential for the diagnosis and treatment of Alzheimer’s disease (AD). Accordingly, systematic investigation of amyloid conformation using analytical tools is essential for precisely quantifying the relative amounts of the three conformations of amyloid peptide. Here, we developed a reduced graphene oxide (rGO) based multiplexing biosensor that could be used to monitor the relative amounts of the three conformations of various amyloid-β 40 (Aβ40) fluids. The electrical rGO biosensor was composed of a multichannel sensor array capable of individual detection of monomers, oligomers, and fibrils in a single amyloid fluid sample. From the performance test of each sensor, we showed that this method had good analytical sensitivity (1 pg/mL) and a fairly wide dynamic range (1 pg/mL to 10 ng/mL) for each conformation of Aβ40. To verify whether the rGO biosensor could be used to evaluate the relative amounts of the three conformations, various amyloid solutions (monomeric Aβ40, aggregated Aβ40, and disaggregated Aβ40 solutions) were employed. Notably, different trends in the relative amounts of the three conformations were observed in each amyloid solution, indicating that this information could serve as an important parameter in the clinical setting. Accordingly, our analytical tool could precisely detect the relative amounts of the three conformations of Aβ40 and may have potential applications as a diagnostic system for AD. MDPI 2018-05-28 /pmc/articles/PMC6022081/ /pubmed/29843431 http://dx.doi.org/10.3390/s18061738 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jeong, Dahye Kim, Jinsik Chae, Myung-Sic Lee, Wonseok Yang, Seung-Hoon Kim, YoungSoo Kim, Seung Min Lee, Jin San Lee, Jeong Hoon Choi, Jungkyu Yoon, Dae Sung Hwang, Kyo Seon Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40 |
title | Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40 |
title_full | Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40 |
title_fullStr | Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40 |
title_full_unstemmed | Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40 |
title_short | Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40 |
title_sort | multifunctionalized reduced graphene oxide biosensors for simultaneous monitoring of structural changes in amyloid-β 40 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022081/ https://www.ncbi.nlm.nih.gov/pubmed/29843431 http://dx.doi.org/10.3390/s18061738 |
work_keys_str_mv | AT jeongdahye multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT kimjinsik multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT chaemyungsic multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT leewonseok multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT yangseunghoon multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT kimyoungsoo multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT kimseungmin multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT leejinsan multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT leejeonghoon multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT choijungkyu multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT yoondaesung multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 AT hwangkyoseon multifunctionalizedreducedgrapheneoxidebiosensorsforsimultaneousmonitoringofstructuralchangesinamyloidb40 |