A Label-Free Aptasensor for Ochratoxin a Detection Based on the Structure Switch of Aptamer

A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-prot...

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Detalles Bibliográficos
Autores principales: Liu, Feng, Ding, Ailing, Zheng, Jiushang, Chen, Jiucun, Wang, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022100/
https://www.ncbi.nlm.nih.gov/pubmed/29857594
http://dx.doi.org/10.3390/s18061769
Descripción
Sumario:A label-free sensing platform is developed based on switching the structure of aptamer for highly sensitive and selective fluorescence detection of ochratoxin A (OTA). OTA induces the structure of aptamer, transforms into G-quadruplex and produces strong fluorescence in the presence of zinc(II)-protoporphyrin IX probe due to the specific bind to G-quadruplex. The simple method exhibits high sensitivity towards OTA with a detection limit of 0.03 nM and excellent selectivity over other mycotoxins. In addition, the successful detection of OTA in real samples represents a promising application in food safety.

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