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User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9

Loss-of-function studies are critically important in gene functional analysis of model organisms and cells. However, conditional gene inactivation in diploid cells is difficult to achieve, as it involves laborious vector construction, multifold electroporation, and complicated genotyping. Here, a st...

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Detalles Bibliográficos
Autores principales:	Chen, Liangliang, Ye, Ying, Dai, Hongxia, Zhang, Heyao, Zhang, Xue, Wu, Qiang, Zhu, Zhexin, Spalinskas, Rapolas, Ren, Wenyan, Zhang, Wensheng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022269/ https://www.ncbi.nlm.nih.gov/pubmed/30013601 http://dx.doi.org/10.1155/2018/9576959

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