Cargando…
Effects of temperature and salinity stress on DNA methylation in a highly invasive marine invertebrate, the colonial ascidian Didemnum vexillum
Environmentally induced epigenetic changes may contribute to phenotypic plasticity, increase adaptive potential in changing environments, and play a key role in the establishment and spread of invasive species in new habitats. In this study, we used methylation-sensitive amplified polymorphism (MSAP...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022722/ https://www.ncbi.nlm.nih.gov/pubmed/29967721 http://dx.doi.org/10.7717/peerj.5003 |
_version_ | 1783335739137720320 |
---|---|
author | Hawes, Nicola A. Tremblay, Louis A. Pochon, Xavier Dunphy, Brendon Fidler, Andrew E. Smith, Kirsty F. |
author_facet | Hawes, Nicola A. Tremblay, Louis A. Pochon, Xavier Dunphy, Brendon Fidler, Andrew E. Smith, Kirsty F. |
author_sort | Hawes, Nicola A. |
collection | PubMed |
description | Environmentally induced epigenetic changes may contribute to phenotypic plasticity, increase adaptive potential in changing environments, and play a key role in the establishment and spread of invasive species in new habitats. In this study, we used methylation-sensitive amplified polymorphism (MSAP) to assess environmentally induced DNA methylation changes in a globally invasive clonal ascidian, Didemnum vexillum. We tested the effect of increasing temperature (19, 25 and 27 °C) and decreasing salinity (34, 32, 30, 28 and 26 practical salinity units (PSU)) on global DNA methylation, growth and survival rates. Exposure to 27 °C resulted in significant changes in DNA methylation over time. Growth also decreased in colonies exposed to high temperatures, suggesting they were under thermal stress. In contrast, no differences in growth nor DNA methylation patterns were observed in colonies exposed to a decreasing salinity gradient, potentially due to prior adaptation. The results of this study show that environmental stress can induce significant global DNA methylation changes in an invasive marine invertebrate on very rapid timescales, and that this response varies depending on the type, magnitude, and duration of the stressor. Changes in genomic DNA methylation and the rate of growth may act to ‘buy survival time’ under stressful conditions, expanding the distribution limits of this globally invasive species. |
format | Online Article Text |
id | pubmed-6022722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-60227222018-07-02 Effects of temperature and salinity stress on DNA methylation in a highly invasive marine invertebrate, the colonial ascidian Didemnum vexillum Hawes, Nicola A. Tremblay, Louis A. Pochon, Xavier Dunphy, Brendon Fidler, Andrew E. Smith, Kirsty F. PeerJ Ecology Environmentally induced epigenetic changes may contribute to phenotypic plasticity, increase adaptive potential in changing environments, and play a key role in the establishment and spread of invasive species in new habitats. In this study, we used methylation-sensitive amplified polymorphism (MSAP) to assess environmentally induced DNA methylation changes in a globally invasive clonal ascidian, Didemnum vexillum. We tested the effect of increasing temperature (19, 25 and 27 °C) and decreasing salinity (34, 32, 30, 28 and 26 practical salinity units (PSU)) on global DNA methylation, growth and survival rates. Exposure to 27 °C resulted in significant changes in DNA methylation over time. Growth also decreased in colonies exposed to high temperatures, suggesting they were under thermal stress. In contrast, no differences in growth nor DNA methylation patterns were observed in colonies exposed to a decreasing salinity gradient, potentially due to prior adaptation. The results of this study show that environmental stress can induce significant global DNA methylation changes in an invasive marine invertebrate on very rapid timescales, and that this response varies depending on the type, magnitude, and duration of the stressor. Changes in genomic DNA methylation and the rate of growth may act to ‘buy survival time’ under stressful conditions, expanding the distribution limits of this globally invasive species. PeerJ Inc. 2018-06-25 /pmc/articles/PMC6022722/ /pubmed/29967721 http://dx.doi.org/10.7717/peerj.5003 Text en ©2018 Hawes et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Ecology Hawes, Nicola A. Tremblay, Louis A. Pochon, Xavier Dunphy, Brendon Fidler, Andrew E. Smith, Kirsty F. Effects of temperature and salinity stress on DNA methylation in a highly invasive marine invertebrate, the colonial ascidian Didemnum vexillum |
title | Effects of temperature and salinity stress on DNA methylation in a highly invasive marine invertebrate, the colonial ascidian Didemnum vexillum |
title_full | Effects of temperature and salinity stress on DNA methylation in a highly invasive marine invertebrate, the colonial ascidian Didemnum vexillum |
title_fullStr | Effects of temperature and salinity stress on DNA methylation in a highly invasive marine invertebrate, the colonial ascidian Didemnum vexillum |
title_full_unstemmed | Effects of temperature and salinity stress on DNA methylation in a highly invasive marine invertebrate, the colonial ascidian Didemnum vexillum |
title_short | Effects of temperature and salinity stress on DNA methylation in a highly invasive marine invertebrate, the colonial ascidian Didemnum vexillum |
title_sort | effects of temperature and salinity stress on dna methylation in a highly invasive marine invertebrate, the colonial ascidian didemnum vexillum |
topic | Ecology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022722/ https://www.ncbi.nlm.nih.gov/pubmed/29967721 http://dx.doi.org/10.7717/peerj.5003 |
work_keys_str_mv | AT hawesnicolaa effectsoftemperatureandsalinitystressondnamethylationinahighlyinvasivemarineinvertebratethecolonialascidiandidemnumvexillum AT tremblaylouisa effectsoftemperatureandsalinitystressondnamethylationinahighlyinvasivemarineinvertebratethecolonialascidiandidemnumvexillum AT pochonxavier effectsoftemperatureandsalinitystressondnamethylationinahighlyinvasivemarineinvertebratethecolonialascidiandidemnumvexillum AT dunphybrendon effectsoftemperatureandsalinitystressondnamethylationinahighlyinvasivemarineinvertebratethecolonialascidiandidemnumvexillum AT fidlerandrewe effectsoftemperatureandsalinitystressondnamethylationinahighlyinvasivemarineinvertebratethecolonialascidiandidemnumvexillum AT smithkirstyf effectsoftemperatureandsalinitystressondnamethylationinahighlyinvasivemarineinvertebratethecolonialascidiandidemnumvexillum |