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Integrative Structure and Functional Anatomy of a Nuclear Pore Complex
Despite the central role of Nuclear Pore Complexes (NPCs) as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm, their large size and dynamic nature have impeded a full structural and functional elucidation. Here, we have determined a subnanometer precision structure for...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022767/ https://www.ncbi.nlm.nih.gov/pubmed/29539637 http://dx.doi.org/10.1038/nature26003 |
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author | Kim, Seung Joong Fernandez-Martinez, Javier Nudelman, Ilona Shi, Yi Zhang, Wenzhu Raveh, Barak Herricks, Thurston Slaughter, Brian D. Hogan, Joanna Upla, Paula Chemmama, Ilan E. Pellarin, Riccardo Echeverria, Ignacia Shivaraju, Manjunatha Chaudhury, Azraa S. Wang, Junjie Williams, Rosemary Unruh, Jay R. Greenberg, Charles H. Jacobs, Erica Y. Yu, Zhiheng de la Cruz, M. Jason Mironska, Roxana Stokes, David L. Aitchison, John D. Jarrold, Martin F. Gerton, Jennifer L. Ludtke, Steven J. Akey, Christopher W. Chait, Brian T. Sali, Andrej Rout, Michael P. |
author_facet | Kim, Seung Joong Fernandez-Martinez, Javier Nudelman, Ilona Shi, Yi Zhang, Wenzhu Raveh, Barak Herricks, Thurston Slaughter, Brian D. Hogan, Joanna Upla, Paula Chemmama, Ilan E. Pellarin, Riccardo Echeverria, Ignacia Shivaraju, Manjunatha Chaudhury, Azraa S. Wang, Junjie Williams, Rosemary Unruh, Jay R. Greenberg, Charles H. Jacobs, Erica Y. Yu, Zhiheng de la Cruz, M. Jason Mironska, Roxana Stokes, David L. Aitchison, John D. Jarrold, Martin F. Gerton, Jennifer L. Ludtke, Steven J. Akey, Christopher W. Chait, Brian T. Sali, Andrej Rout, Michael P. |
author_sort | Kim, Seung Joong |
collection | PubMed |
description | Despite the central role of Nuclear Pore Complexes (NPCs) as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm, their large size and dynamic nature have impeded a full structural and functional elucidation. Here, we have determined a subnanometer precision structure for the entire 552-protein yeast NPC by satisfying diverse data including stoichiometry, a cryo-electron tomography map, and chemical cross-links. The structure reveals the NPC’s functional elements in unprecedented detail. The NPC is built of sturdy diagonal columns to which are attached connector cables, imbuing both strength and flexibility, while tying together all other elements of the NPC, including membrane-interacting regions and RNA processing platforms. Inwardly-directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized in distinct functional units. Taken together, this integrative structure allows us to rationalize the architecture, transport mechanism, and evolutionary origins of the NPC. |
format | Online Article Text |
id | pubmed-6022767 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
record_format | MEDLINE/PubMed |
spelling | pubmed-60227672018-09-14 Integrative Structure and Functional Anatomy of a Nuclear Pore Complex Kim, Seung Joong Fernandez-Martinez, Javier Nudelman, Ilona Shi, Yi Zhang, Wenzhu Raveh, Barak Herricks, Thurston Slaughter, Brian D. Hogan, Joanna Upla, Paula Chemmama, Ilan E. Pellarin, Riccardo Echeverria, Ignacia Shivaraju, Manjunatha Chaudhury, Azraa S. Wang, Junjie Williams, Rosemary Unruh, Jay R. Greenberg, Charles H. Jacobs, Erica Y. Yu, Zhiheng de la Cruz, M. Jason Mironska, Roxana Stokes, David L. Aitchison, John D. Jarrold, Martin F. Gerton, Jennifer L. Ludtke, Steven J. Akey, Christopher W. Chait, Brian T. Sali, Andrej Rout, Michael P. Nature Article Despite the central role of Nuclear Pore Complexes (NPCs) as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm, their large size and dynamic nature have impeded a full structural and functional elucidation. Here, we have determined a subnanometer precision structure for the entire 552-protein yeast NPC by satisfying diverse data including stoichiometry, a cryo-electron tomography map, and chemical cross-links. The structure reveals the NPC’s functional elements in unprecedented detail. The NPC is built of sturdy diagonal columns to which are attached connector cables, imbuing both strength and flexibility, while tying together all other elements of the NPC, including membrane-interacting regions and RNA processing platforms. Inwardly-directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized in distinct functional units. Taken together, this integrative structure allows us to rationalize the architecture, transport mechanism, and evolutionary origins of the NPC. 2018-03-14 2018-03-22 /pmc/articles/PMC6022767/ /pubmed/29539637 http://dx.doi.org/10.1038/nature26003 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms Reprints and permissions information is available at www.nature.com/reprints (http://www.nature.com/reprints) . |
spellingShingle | Article Kim, Seung Joong Fernandez-Martinez, Javier Nudelman, Ilona Shi, Yi Zhang, Wenzhu Raveh, Barak Herricks, Thurston Slaughter, Brian D. Hogan, Joanna Upla, Paula Chemmama, Ilan E. Pellarin, Riccardo Echeverria, Ignacia Shivaraju, Manjunatha Chaudhury, Azraa S. Wang, Junjie Williams, Rosemary Unruh, Jay R. Greenberg, Charles H. Jacobs, Erica Y. Yu, Zhiheng de la Cruz, M. Jason Mironska, Roxana Stokes, David L. Aitchison, John D. Jarrold, Martin F. Gerton, Jennifer L. Ludtke, Steven J. Akey, Christopher W. Chait, Brian T. Sali, Andrej Rout, Michael P. Integrative Structure and Functional Anatomy of a Nuclear Pore Complex |
title | Integrative Structure and Functional Anatomy of a Nuclear Pore Complex |
title_full | Integrative Structure and Functional Anatomy of a Nuclear Pore Complex |
title_fullStr | Integrative Structure and Functional Anatomy of a Nuclear Pore Complex |
title_full_unstemmed | Integrative Structure and Functional Anatomy of a Nuclear Pore Complex |
title_short | Integrative Structure and Functional Anatomy of a Nuclear Pore Complex |
title_sort | integrative structure and functional anatomy of a nuclear pore complex |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022767/ https://www.ncbi.nlm.nih.gov/pubmed/29539637 http://dx.doi.org/10.1038/nature26003 |
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