Unique Raman Spectroscopic Fingerprints of B-Cell Non-Hodgkin Lymphoma: Implications for Diagnosis, Prognosis and New Therapies

OBJECTIVE: Raman spectroscopy is a non-invasive laser-based technique that identifies molecular chemical composition of tissues and cells. The objective of the work was to demonstrate that unique Raman spectroscopic fingerprints of B-cell non-Hodgkin lymphoma cells could be distinguished from normal...

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Autores principales: Shiramizu, B, Oda, R, Kamada, N, Garcia, MA, Shieh, T, Maeda, TA, Choi, SY, Lim, E, Misra, A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022842/
https://www.ncbi.nlm.nih.gov/pubmed/29962510
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author Shiramizu, B
Oda, R
Kamada, N
Garcia, MA
Shieh, T
Maeda, TA
Choi, SY
Lim, E
Misra, A
author_facet Shiramizu, B
Oda, R
Kamada, N
Garcia, MA
Shieh, T
Maeda, TA
Choi, SY
Lim, E
Misra, A
author_sort Shiramizu, B
collection PubMed
description OBJECTIVE: Raman spectroscopy is a non-invasive laser-based technique that identifies molecular chemical composition of tissues and cells. The objective of the work was to demonstrate that unique Raman spectroscopic fingerprints of B-cell non-Hodgkin lymphoma cells could be distinguished from normal B-cells. METHODS: Normal B-cells and B-cell non-Hodgkin lymphoma cells were mounted on aluminum slides and analyzed by Raman spectroscopy using Asymmetric Least Squares and Principal Component Analysis. RESULTS: Clustering by Principal Component Analysis differentiated normal B-cells from B-cell non-Hodgkin lymphoma cells as well as between the different B-cell non-Hodgkin lymphoma cell types. CONCLUSIONS: Raman spectroscopy technology provided a different paradigm in analyzing tumor cells which could be used for diagnosis as well as contribute new information on unique characteristics of cancer cells to understand pathogenesis and potential novel treatments.
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spelling pubmed-60228422018-06-28 Unique Raman Spectroscopic Fingerprints of B-Cell Non-Hodgkin Lymphoma: Implications for Diagnosis, Prognosis and New Therapies Shiramizu, B Oda, R Kamada, N Garcia, MA Shieh, T Maeda, TA Choi, SY Lim, E Misra, A J Biol Med Sci Article OBJECTIVE: Raman spectroscopy is a non-invasive laser-based technique that identifies molecular chemical composition of tissues and cells. The objective of the work was to demonstrate that unique Raman spectroscopic fingerprints of B-cell non-Hodgkin lymphoma cells could be distinguished from normal B-cells. METHODS: Normal B-cells and B-cell non-Hodgkin lymphoma cells were mounted on aluminum slides and analyzed by Raman spectroscopy using Asymmetric Least Squares and Principal Component Analysis. RESULTS: Clustering by Principal Component Analysis differentiated normal B-cells from B-cell non-Hodgkin lymphoma cells as well as between the different B-cell non-Hodgkin lymphoma cell types. CONCLUSIONS: Raman spectroscopy technology provided a different paradigm in analyzing tumor cells which could be used for diagnosis as well as contribute new information on unique characteristics of cancer cells to understand pathogenesis and potential novel treatments. 2018-01-18 2018 /pmc/articles/PMC6022842/ /pubmed/29962510 Text en http://creativecommons.org/licenses/by/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Shiramizu, B
Oda, R
Kamada, N
Garcia, MA
Shieh, T
Maeda, TA
Choi, SY
Lim, E
Misra, A
Unique Raman Spectroscopic Fingerprints of B-Cell Non-Hodgkin Lymphoma: Implications for Diagnosis, Prognosis and New Therapies
title Unique Raman Spectroscopic Fingerprints of B-Cell Non-Hodgkin Lymphoma: Implications for Diagnosis, Prognosis and New Therapies
title_full Unique Raman Spectroscopic Fingerprints of B-Cell Non-Hodgkin Lymphoma: Implications for Diagnosis, Prognosis and New Therapies
title_fullStr Unique Raman Spectroscopic Fingerprints of B-Cell Non-Hodgkin Lymphoma: Implications for Diagnosis, Prognosis and New Therapies
title_full_unstemmed Unique Raman Spectroscopic Fingerprints of B-Cell Non-Hodgkin Lymphoma: Implications for Diagnosis, Prognosis and New Therapies
title_short Unique Raman Spectroscopic Fingerprints of B-Cell Non-Hodgkin Lymphoma: Implications for Diagnosis, Prognosis and New Therapies
title_sort unique raman spectroscopic fingerprints of b-cell non-hodgkin lymphoma: implications for diagnosis, prognosis and new therapies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022842/
https://www.ncbi.nlm.nih.gov/pubmed/29962510
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