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Droplet Velocity Measurement Based on Dielectric Layer Thickness Variation Using Digital Microfluidic Devices

In recent years, the number of interdisciplinary research works related to the development of miniaturized systems with integrated chemical and biological analyses is increasing. Digital microfluidic biochips (DMFBs) are one kind of miniaturized systems designed for conducting inexpensive, fast, con...

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Autores principales: Zulkepli, Siti Noor Idora Syafinaz, Hamid, Nor Hisham, Shukla, Vineeta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022883/
https://www.ncbi.nlm.nih.gov/pubmed/29738428
http://dx.doi.org/10.3390/bios8020045
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author Zulkepli, Siti Noor Idora Syafinaz
Hamid, Nor Hisham
Shukla, Vineeta
author_facet Zulkepli, Siti Noor Idora Syafinaz
Hamid, Nor Hisham
Shukla, Vineeta
author_sort Zulkepli, Siti Noor Idora Syafinaz
collection PubMed
description In recent years, the number of interdisciplinary research works related to the development of miniaturized systems with integrated chemical and biological analyses is increasing. Digital microfluidic biochips (DMFBs) are one kind of miniaturized systems designed for conducting inexpensive, fast, convenient and reliable biochemical assay procedures focusing on basic scientific research and medical diagnostics. The role of a dielectric layer in the digital microfluidic biochips is prominent as it helps in actuating microliter droplets based on the electrowetting-on-dielectric (EWOD) technique. The advantages of using three different material layers of dielectric such as parafilm, polytetrafluoroethylene (PTFE) and ethylene tetrafluoroethylene (ETFE) were reported in the current work. A simple fabrication process of a digital microfluidic device was performed and good results were obtained. The threshold of the actuation voltage was determined for all dielectric materials of varying thicknesses. Additionally, the OpenDrop device was tested by utilizing a single-plate system to transport microliter droplets for a bioassay operation. With the newly proposed fabrication methods, these dielectric materials showed changes in contact angle and droplet velocity when the actuation voltage was applied. The threshold actuation voltage for the dielectric layers of 10–13 μm was 190 V for the open plate DMFBs.
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spelling pubmed-60228832018-07-02 Droplet Velocity Measurement Based on Dielectric Layer Thickness Variation Using Digital Microfluidic Devices Zulkepli, Siti Noor Idora Syafinaz Hamid, Nor Hisham Shukla, Vineeta Biosensors (Basel) Article In recent years, the number of interdisciplinary research works related to the development of miniaturized systems with integrated chemical and biological analyses is increasing. Digital microfluidic biochips (DMFBs) are one kind of miniaturized systems designed for conducting inexpensive, fast, convenient and reliable biochemical assay procedures focusing on basic scientific research and medical diagnostics. The role of a dielectric layer in the digital microfluidic biochips is prominent as it helps in actuating microliter droplets based on the electrowetting-on-dielectric (EWOD) technique. The advantages of using three different material layers of dielectric such as parafilm, polytetrafluoroethylene (PTFE) and ethylene tetrafluoroethylene (ETFE) were reported in the current work. A simple fabrication process of a digital microfluidic device was performed and good results were obtained. The threshold of the actuation voltage was determined for all dielectric materials of varying thicknesses. Additionally, the OpenDrop device was tested by utilizing a single-plate system to transport microliter droplets for a bioassay operation. With the newly proposed fabrication methods, these dielectric materials showed changes in contact angle and droplet velocity when the actuation voltage was applied. The threshold actuation voltage for the dielectric layers of 10–13 μm was 190 V for the open plate DMFBs. MDPI 2018-05-08 /pmc/articles/PMC6022883/ /pubmed/29738428 http://dx.doi.org/10.3390/bios8020045 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zulkepli, Siti Noor Idora Syafinaz
Hamid, Nor Hisham
Shukla, Vineeta
Droplet Velocity Measurement Based on Dielectric Layer Thickness Variation Using Digital Microfluidic Devices
title Droplet Velocity Measurement Based on Dielectric Layer Thickness Variation Using Digital Microfluidic Devices
title_full Droplet Velocity Measurement Based on Dielectric Layer Thickness Variation Using Digital Microfluidic Devices
title_fullStr Droplet Velocity Measurement Based on Dielectric Layer Thickness Variation Using Digital Microfluidic Devices
title_full_unstemmed Droplet Velocity Measurement Based on Dielectric Layer Thickness Variation Using Digital Microfluidic Devices
title_short Droplet Velocity Measurement Based on Dielectric Layer Thickness Variation Using Digital Microfluidic Devices
title_sort droplet velocity measurement based on dielectric layer thickness variation using digital microfluidic devices
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022883/
https://www.ncbi.nlm.nih.gov/pubmed/29738428
http://dx.doi.org/10.3390/bios8020045
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