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Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes
Glycopeptide antibiotics are drugs of last resort for treating severe infections caused by Gram-positive pathogens. It is widely believed that glycopeptide-resistance determinants (van genes) are ultimately derived from the producing actinomycetes. We hereby investigated the relationship between the...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022977/ https://www.ncbi.nlm.nih.gov/pubmed/29693566 http://dx.doi.org/10.3390/antibiotics7020036 |
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author | Binda, Elisa Cappelletti, Pamela Marinelli, Flavia Marcone, Giorgia Letizia |
author_facet | Binda, Elisa Cappelletti, Pamela Marinelli, Flavia Marcone, Giorgia Letizia |
author_sort | Binda, Elisa |
collection | PubMed |
description | Glycopeptide antibiotics are drugs of last resort for treating severe infections caused by Gram-positive pathogens. It is widely believed that glycopeptide-resistance determinants (van genes) are ultimately derived from the producing actinomycetes. We hereby investigated the relationship between the antimicrobial activity of vancomycin and teicoplanins and their differential ability to induce van gene expression in Actinoplanes teichomyceticus—the producer of teicoplanin—and Nonomuraea gerenzanensis—the producer of the teicoplanin-like A40926. As a control, we used the well-characterized resistance model Streptomyces coelicolor. The enzyme activities of a cytoplasmic-soluble d,d-dipeptidase and of a membrane-associated d,d-carboxypeptidase (corresponding to VanX and VanY respectively) involved in resistant cell wall remodeling were measured in the actinomycetes grown in the presence or absence of subinhibitory concentrations of vancomycin, teicoplanin, and A40926. Results indicated that actinomycetes possess diverse self-resistance mechanisms, and that each of them responds differently to glycopeptide induction. Gene swapping among teicoplanins-producing actinomycetes indicated that cross-talking is possible and provides useful information for predicting the evolution of future resistance gene combinations emerging in pathogens. |
format | Online Article Text |
id | pubmed-6022977 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60229772018-07-02 Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes Binda, Elisa Cappelletti, Pamela Marinelli, Flavia Marcone, Giorgia Letizia Antibiotics (Basel) Article Glycopeptide antibiotics are drugs of last resort for treating severe infections caused by Gram-positive pathogens. It is widely believed that glycopeptide-resistance determinants (van genes) are ultimately derived from the producing actinomycetes. We hereby investigated the relationship between the antimicrobial activity of vancomycin and teicoplanins and their differential ability to induce van gene expression in Actinoplanes teichomyceticus—the producer of teicoplanin—and Nonomuraea gerenzanensis—the producer of the teicoplanin-like A40926. As a control, we used the well-characterized resistance model Streptomyces coelicolor. The enzyme activities of a cytoplasmic-soluble d,d-dipeptidase and of a membrane-associated d,d-carboxypeptidase (corresponding to VanX and VanY respectively) involved in resistant cell wall remodeling were measured in the actinomycetes grown in the presence or absence of subinhibitory concentrations of vancomycin, teicoplanin, and A40926. Results indicated that actinomycetes possess diverse self-resistance mechanisms, and that each of them responds differently to glycopeptide induction. Gene swapping among teicoplanins-producing actinomycetes indicated that cross-talking is possible and provides useful information for predicting the evolution of future resistance gene combinations emerging in pathogens. MDPI 2018-04-25 /pmc/articles/PMC6022977/ /pubmed/29693566 http://dx.doi.org/10.3390/antibiotics7020036 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Binda, Elisa Cappelletti, Pamela Marinelli, Flavia Marcone, Giorgia Letizia Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes |
title | Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes |
title_full | Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes |
title_fullStr | Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes |
title_full_unstemmed | Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes |
title_short | Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes |
title_sort | specificity of induction of glycopeptide antibiotic resistance in the producing actinomycetes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022977/ https://www.ncbi.nlm.nih.gov/pubmed/29693566 http://dx.doi.org/10.3390/antibiotics7020036 |
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