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Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes

Glycopeptide antibiotics are drugs of last resort for treating severe infections caused by Gram-positive pathogens. It is widely believed that glycopeptide-resistance determinants (van genes) are ultimately derived from the producing actinomycetes. We hereby investigated the relationship between the...

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Autores principales: Binda, Elisa, Cappelletti, Pamela, Marinelli, Flavia, Marcone, Giorgia Letizia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022977/
https://www.ncbi.nlm.nih.gov/pubmed/29693566
http://dx.doi.org/10.3390/antibiotics7020036
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author Binda, Elisa
Cappelletti, Pamela
Marinelli, Flavia
Marcone, Giorgia Letizia
author_facet Binda, Elisa
Cappelletti, Pamela
Marinelli, Flavia
Marcone, Giorgia Letizia
author_sort Binda, Elisa
collection PubMed
description Glycopeptide antibiotics are drugs of last resort for treating severe infections caused by Gram-positive pathogens. It is widely believed that glycopeptide-resistance determinants (van genes) are ultimately derived from the producing actinomycetes. We hereby investigated the relationship between the antimicrobial activity of vancomycin and teicoplanins and their differential ability to induce van gene expression in Actinoplanes teichomyceticus—the producer of teicoplanin—and Nonomuraea gerenzanensis—the producer of the teicoplanin-like A40926. As a control, we used the well-characterized resistance model Streptomyces coelicolor. The enzyme activities of a cytoplasmic-soluble d,d-dipeptidase and of a membrane-associated d,d-carboxypeptidase (corresponding to VanX and VanY respectively) involved in resistant cell wall remodeling were measured in the actinomycetes grown in the presence or absence of subinhibitory concentrations of vancomycin, teicoplanin, and A40926. Results indicated that actinomycetes possess diverse self-resistance mechanisms, and that each of them responds differently to glycopeptide induction. Gene swapping among teicoplanins-producing actinomycetes indicated that cross-talking is possible and provides useful information for predicting the evolution of future resistance gene combinations emerging in pathogens.
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spelling pubmed-60229772018-07-02 Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes Binda, Elisa Cappelletti, Pamela Marinelli, Flavia Marcone, Giorgia Letizia Antibiotics (Basel) Article Glycopeptide antibiotics are drugs of last resort for treating severe infections caused by Gram-positive pathogens. It is widely believed that glycopeptide-resistance determinants (van genes) are ultimately derived from the producing actinomycetes. We hereby investigated the relationship between the antimicrobial activity of vancomycin and teicoplanins and their differential ability to induce van gene expression in Actinoplanes teichomyceticus—the producer of teicoplanin—and Nonomuraea gerenzanensis—the producer of the teicoplanin-like A40926. As a control, we used the well-characterized resistance model Streptomyces coelicolor. The enzyme activities of a cytoplasmic-soluble d,d-dipeptidase and of a membrane-associated d,d-carboxypeptidase (corresponding to VanX and VanY respectively) involved in resistant cell wall remodeling were measured in the actinomycetes grown in the presence or absence of subinhibitory concentrations of vancomycin, teicoplanin, and A40926. Results indicated that actinomycetes possess diverse self-resistance mechanisms, and that each of them responds differently to glycopeptide induction. Gene swapping among teicoplanins-producing actinomycetes indicated that cross-talking is possible and provides useful information for predicting the evolution of future resistance gene combinations emerging in pathogens. MDPI 2018-04-25 /pmc/articles/PMC6022977/ /pubmed/29693566 http://dx.doi.org/10.3390/antibiotics7020036 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Binda, Elisa
Cappelletti, Pamela
Marinelli, Flavia
Marcone, Giorgia Letizia
Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes
title Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes
title_full Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes
title_fullStr Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes
title_full_unstemmed Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes
title_short Specificity of Induction of Glycopeptide Antibiotic Resistance in the Producing Actinomycetes
title_sort specificity of induction of glycopeptide antibiotic resistance in the producing actinomycetes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6022977/
https://www.ncbi.nlm.nih.gov/pubmed/29693566
http://dx.doi.org/10.3390/antibiotics7020036
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