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S-Layer Protein-Based Biosensors
The present paper highlights the application of bacterial surface (S-) layer proteins as versatile components for the fabrication of biosensors. One technologically relevant feature of S-layer proteins is their ability to self-assemble on many surfaces and interfaces to form a crystalline two-dimens...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2018
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023001/ https://www.ncbi.nlm.nih.gov/pubmed/29641511 http://dx.doi.org/10.3390/bios8020040 |
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author | Schuster, Bernhard |
author_facet | Schuster, Bernhard |
author_sort | Schuster, Bernhard |
collection | PubMed |
description | The present paper highlights the application of bacterial surface (S-) layer proteins as versatile components for the fabrication of biosensors. One technologically relevant feature of S-layer proteins is their ability to self-assemble on many surfaces and interfaces to form a crystalline two-dimensional (2D) protein lattice. The S-layer lattice on the surface of a biosensor becomes part of the interface architecture linking the bioreceptor to the transducer interface, which may cause signal amplification. The S-layer lattice as ultrathin, highly porous structure with functional groups in a well-defined special distribution and orientation and an overall anti-fouling characteristics can significantly raise the limit in terms of variety and the ease of bioreceptor immobilization, compactness of bioreceptor molecule arrangement, sensitivity, specificity, and detection limit for many types of biosensors. The present paper discusses and summarizes examples for the successful implementation of S-layer lattices on biosensor surfaces in order to give a comprehensive overview on the application potential of these bioinspired S-layer protein-based biosensors. |
format | Online Article Text |
id | pubmed-6023001 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-60230012018-07-02 S-Layer Protein-Based Biosensors Schuster, Bernhard Biosensors (Basel) Article The present paper highlights the application of bacterial surface (S-) layer proteins as versatile components for the fabrication of biosensors. One technologically relevant feature of S-layer proteins is their ability to self-assemble on many surfaces and interfaces to form a crystalline two-dimensional (2D) protein lattice. The S-layer lattice on the surface of a biosensor becomes part of the interface architecture linking the bioreceptor to the transducer interface, which may cause signal amplification. The S-layer lattice as ultrathin, highly porous structure with functional groups in a well-defined special distribution and orientation and an overall anti-fouling characteristics can significantly raise the limit in terms of variety and the ease of bioreceptor immobilization, compactness of bioreceptor molecule arrangement, sensitivity, specificity, and detection limit for many types of biosensors. The present paper discusses and summarizes examples for the successful implementation of S-layer lattices on biosensor surfaces in order to give a comprehensive overview on the application potential of these bioinspired S-layer protein-based biosensors. MDPI 2018-04-11 /pmc/articles/PMC6023001/ /pubmed/29641511 http://dx.doi.org/10.3390/bios8020040 Text en © 2018 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Schuster, Bernhard S-Layer Protein-Based Biosensors |
title | S-Layer Protein-Based Biosensors |
title_full | S-Layer Protein-Based Biosensors |
title_fullStr | S-Layer Protein-Based Biosensors |
title_full_unstemmed | S-Layer Protein-Based Biosensors |
title_short | S-Layer Protein-Based Biosensors |
title_sort | s-layer protein-based biosensors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6023001/ https://www.ncbi.nlm.nih.gov/pubmed/29641511 http://dx.doi.org/10.3390/bios8020040 |
work_keys_str_mv | AT schusterbernhard slayerproteinbasedbiosensors |